Pregnancy Rate after Intrauterine Insemination with the Presence or Absence of Leukocytospermia in Sperms Prepared using Density Gradient Method
Objective: To examine the association between different concentrations of leukocyte and sperm recovery rate after sperms are prepared using density gradient method and pregnancy rate after intrauterine insemination (IUI). Increased leukocytes in semen have been associated with increased reactive oxy...
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Universitas Padjadjaran
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doaj-35e15ec735344371bf35418f9453fd502020-11-25T00:48:01ZengUniversitas PadjadjaranInternational Journal of Integrated Health Sciences2302-13812338-45062018-09-0162636610.15850/ijihs.v6n2.1318Pregnancy Rate after Intrauterine Insemination with the Presence or Absence of Leukocytospermia in Sperms Prepared using Density Gradient MethodRini Widyastuti0Julius Pangayoman1Aida Riyanti2Alkaustariyah Lubis3Mas Rizky Anggun Adipurna Syamsunarno4Study Program of Veterinary Medicine, Faculty of Medicine, Universitas PadjadjaranMelinda HospitalIndonesia Reproductive Medicine Research and Training Center-Indonesia Medical Research and Education InstituteFaculty of Medicine, Universitas PadjadjaranDivision of Biochemistry and Molecular Biology, Departement of Biomedicine Sciences, Faculty of Medicine, Universitas PadjadjaranObjective: To examine the association between different concentrations of leukocyte and sperm recovery rate after sperms are prepared using density gradient method and pregnancy rate after intrauterine insemination (IUI). Increased leukocytes in semen have been associated with increased reactive oxygen species (ROS) that reduces sperm quality. Methods: Semen samples that were collected from 31 male partners of couples undergoing infertility investigation were analyzed for sperm concentration, motility, and leucocytes concentration. Semen samples were then divided in two groups based on their leucocytes concentrations (category A: >0 to <1 × 106/mL; category B: >1 x 106/mL. Semen samples were processed using density-gradient centrifugation technique. Results: There was a significant difference in the number of sperms harvested and sperm motility after preparation. Interestingly, pregnancy rate after IUI was higher (p<0.05) in non-leukocytospermia semen (39%) when compared to leukocytospermia semen (30%). Conclusions: Seminal leukocytes (PMNL) concentration affects pregnancy rate after intrauterine insemination.http://journal.fk.unpad.ac.id/index.php/ijihs/article/view/1318Density gradient methodsperm recovery rateintrauterine inseminationpregnancy rate |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rini Widyastuti Julius Pangayoman Aida Riyanti Alkaustariyah Lubis Mas Rizky Anggun Adipurna Syamsunarno |
spellingShingle |
Rini Widyastuti Julius Pangayoman Aida Riyanti Alkaustariyah Lubis Mas Rizky Anggun Adipurna Syamsunarno Pregnancy Rate after Intrauterine Insemination with the Presence or Absence of Leukocytospermia in Sperms Prepared using Density Gradient Method International Journal of Integrated Health Sciences Density gradient method sperm recovery rate intrauterine insemination pregnancy rate |
author_facet |
Rini Widyastuti Julius Pangayoman Aida Riyanti Alkaustariyah Lubis Mas Rizky Anggun Adipurna Syamsunarno |
author_sort |
Rini Widyastuti |
title |
Pregnancy Rate after Intrauterine Insemination with the Presence or Absence of Leukocytospermia in Sperms Prepared using Density Gradient Method |
title_short |
Pregnancy Rate after Intrauterine Insemination with the Presence or Absence of Leukocytospermia in Sperms Prepared using Density Gradient Method |
title_full |
Pregnancy Rate after Intrauterine Insemination with the Presence or Absence of Leukocytospermia in Sperms Prepared using Density Gradient Method |
title_fullStr |
Pregnancy Rate after Intrauterine Insemination with the Presence or Absence of Leukocytospermia in Sperms Prepared using Density Gradient Method |
title_full_unstemmed |
Pregnancy Rate after Intrauterine Insemination with the Presence or Absence of Leukocytospermia in Sperms Prepared using Density Gradient Method |
title_sort |
pregnancy rate after intrauterine insemination with the presence or absence of leukocytospermia in sperms prepared using density gradient method |
publisher |
Universitas Padjadjaran |
series |
International Journal of Integrated Health Sciences |
issn |
2302-1381 2338-4506 |
publishDate |
2018-09-01 |
description |
Objective: To examine the association between different concentrations of leukocyte and sperm recovery rate after sperms are prepared using density gradient method and pregnancy rate after intrauterine insemination (IUI). Increased leukocytes in semen have been associated with increased reactive oxygen species (ROS) that reduces sperm quality.
Methods: Semen samples that were collected from 31 male partners of couples undergoing infertility investigation were analyzed for sperm concentration, motility, and leucocytes concentration. Semen samples were then divided in two groups based on their leucocytes concentrations (category A: >0 to <1 × 106/mL; category B: >1 x 106/mL. Semen samples were processed using density-gradient centrifugation technique.
Results: There was a significant difference in the number of sperms harvested and sperm motility after preparation. Interestingly, pregnancy rate after IUI was higher (p<0.05) in non-leukocytospermia semen (39%) when compared to leukocytospermia semen (30%).
Conclusions: Seminal leukocytes (PMNL) concentration affects pregnancy rate after intrauterine insemination. |
topic |
Density gradient method sperm recovery rate intrauterine insemination pregnancy rate |
url |
http://journal.fk.unpad.ac.id/index.php/ijihs/article/view/1318 |
work_keys_str_mv |
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