Early Appearance of TNF-α and Other Cytokines in Bronchus Associated Lymphoid Tissues (BALT) from Growing Wistar Rats. What is the Role of TNF-α?
Several different cytokines trigger the development of determined cell subsets in BALT of growing Wistar rats. Early appearance (4 days post partum) of γδT cells in BALT has been shown, as well as its role in up-regulating TNF-α production. In the present report,...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Hindawi Limited
2004-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1080/17402520400001686 |
| Summary: | Several different cytokines trigger the development of determined cell subsets
in BALT of growing Wistar rats. Early appearance (4 days post partum) of γδT cells in
BALT has been shown, as well as its role in up-regulating TNF-α production. In the
present report, we studied in the BALT: (1) the profile of the cytokines, TNF-α, INF-γ
and IL-10 and (2) in TCR γδ+ cells, the existence of a colocalization with TNF-α as well
as with INF-γ. All the cytokines studied were observed at an early stage of BALT
development by immunohistochemistry and in bronchoalveolar cells (BAL cells) by
flow cytometry and western blot. (1) The principal cytokine found at 4 days of age in
BALT cells was TNF-α that increases along BALT development. The same behavior
was found for cells containing IL-10 and INF-γ. (2) TCR γδ+ cells colocalize mainly with
TNF-α as it has been shown by immunohistochemistry in BALT and by flow cytometry
when we studied BAL. |
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| ISSN: | 1740-2522 1740-2530 |