Comparison of Potato and Asian Citrus Psyllid Adult and Nymph Transcriptomes Identified Vector Transcripts with Potential Involvement in Circulative, Propagative Liberibacter Transmission
The potato psyllid (PoP) Bactericera cockerelli (Sulc) and Asian citrus psyllid (ACP) Diaphorina citri Kuwayama are the insect vectors of the fastidious plant pathogen, Candidatus Liberibacter solanacearum (CLso) and Ca. L. asiaticus (CLas), respectively. CLso causes Zebra chip disease of potato and...
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doaj-35f8f976f1274f589be249f0c7d44a612020-11-24T23:14:29ZengMDPI AGPathogens2076-08172014-11-013487590710.3390/pathogens3040875pathogens3040875Comparison of Potato and Asian Citrus Psyllid Adult and Nymph Transcriptomes Identified Vector Transcripts with Potential Involvement in Circulative, Propagative Liberibacter TransmissionTonja W. Fisher0Meenal Vyas1Ruifeng He2William Nelson3Joseph M. Cicero4Mark Willer5Ryan Kim6Robin Kramer7Greg A. May8John A. Crow9Carol A. Soderlund10David R. Gang11Judith K. Brown12School of Plant Sciences, The University of Arizona, Tucson, AZ 85721, USASchool of Plant Sciences, The University of Arizona, Tucson, AZ 85721, USAInstitute of Biological Chemistry, Washington State University, Pullman, WA 99164, USABIO5, The University of Arizona, Tucson, AZ 85721, USASchool of Plant Sciences, The University of Arizona, Tucson, AZ 85721, USABIO5, The University of Arizona, Tucson, AZ 85721, USANational Center for Genome Resources, 2935 Rodeo Park Drive East, Santa Fe, NM 87505, USANational Center for Genome Resources, 2935 Rodeo Park Drive East, Santa Fe, NM 87505, USANational Center for Genome Resources, 2935 Rodeo Park Drive East, Santa Fe, NM 87505, USANational Center for Genome Resources, 2935 Rodeo Park Drive East, Santa Fe, NM 87505, USABIO5, The University of Arizona, Tucson, AZ 85721, USAInstitute of Biological Chemistry, Washington State University, Pullman, WA 99164, USASchool of Plant Sciences, The University of Arizona, Tucson, AZ 85721, USAThe potato psyllid (PoP) Bactericera cockerelli (Sulc) and Asian citrus psyllid (ACP) Diaphorina citri Kuwayama are the insect vectors of the fastidious plant pathogen, Candidatus Liberibacter solanacearum (CLso) and Ca. L. asiaticus (CLas), respectively. CLso causes Zebra chip disease of potato and vein-greening in solanaceous species, whereas, CLas causes citrus greening disease. The reliance on insecticides for vector management to reduce pathogen transmission has increased interest in alternative approaches, including RNA interference to abate expression of genes essential for psyllid-mediated Ca. Liberibacter transmission. To identify genes with significantly altered expression at different life stages and conditions of CLso/CLas infection, cDNA libraries were constructed for CLso-infected and -uninfected PoP adults and nymphal instars. Illumina sequencing produced 199,081,451 reads that were assembled into 82,224 unique transcripts. PoP and the analogous transcripts from ACP adult and nymphs reported elsewhere were annotated, organized into functional gene groups using the Gene Ontology classification system, and analyzed for differential in silico expression. Expression profiles revealed vector life stage differences and differential gene expression associated with Liberibacter infection of the psyllid host, including invasion, immune system modulation, nutrition, and development.http://www.mdpi.com/2076-0817/3/4/875circulative-propagative transmissionfastidious plant bacteriapsyllid vectortranscriptome |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tonja W. Fisher Meenal Vyas Ruifeng He William Nelson Joseph M. Cicero Mark Willer Ryan Kim Robin Kramer Greg A. May John A. Crow Carol A. Soderlund David R. Gang Judith K. Brown |
spellingShingle |
Tonja W. Fisher Meenal Vyas Ruifeng He William Nelson Joseph M. Cicero Mark Willer Ryan Kim Robin Kramer Greg A. May John A. Crow Carol A. Soderlund David R. Gang Judith K. Brown Comparison of Potato and Asian Citrus Psyllid Adult and Nymph Transcriptomes Identified Vector Transcripts with Potential Involvement in Circulative, Propagative Liberibacter Transmission Pathogens circulative-propagative transmission fastidious plant bacteria psyllid vector transcriptome |
author_facet |
Tonja W. Fisher Meenal Vyas Ruifeng He William Nelson Joseph M. Cicero Mark Willer Ryan Kim Robin Kramer Greg A. May John A. Crow Carol A. Soderlund David R. Gang Judith K. Brown |
author_sort |
Tonja W. Fisher |
title |
Comparison of Potato and Asian Citrus Psyllid Adult and Nymph Transcriptomes Identified Vector Transcripts with Potential Involvement in Circulative, Propagative Liberibacter Transmission |
title_short |
Comparison of Potato and Asian Citrus Psyllid Adult and Nymph Transcriptomes Identified Vector Transcripts with Potential Involvement in Circulative, Propagative Liberibacter Transmission |
title_full |
Comparison of Potato and Asian Citrus Psyllid Adult and Nymph Transcriptomes Identified Vector Transcripts with Potential Involvement in Circulative, Propagative Liberibacter Transmission |
title_fullStr |
Comparison of Potato and Asian Citrus Psyllid Adult and Nymph Transcriptomes Identified Vector Transcripts with Potential Involvement in Circulative, Propagative Liberibacter Transmission |
title_full_unstemmed |
Comparison of Potato and Asian Citrus Psyllid Adult and Nymph Transcriptomes Identified Vector Transcripts with Potential Involvement in Circulative, Propagative Liberibacter Transmission |
title_sort |
comparison of potato and asian citrus psyllid adult and nymph transcriptomes identified vector transcripts with potential involvement in circulative, propagative liberibacter transmission |
publisher |
MDPI AG |
series |
Pathogens |
issn |
2076-0817 |
publishDate |
2014-11-01 |
description |
The potato psyllid (PoP) Bactericera cockerelli (Sulc) and Asian citrus psyllid (ACP) Diaphorina citri Kuwayama are the insect vectors of the fastidious plant pathogen, Candidatus Liberibacter solanacearum (CLso) and Ca. L. asiaticus (CLas), respectively. CLso causes Zebra chip disease of potato and vein-greening in solanaceous species, whereas, CLas causes citrus greening disease. The reliance on insecticides for vector management to reduce pathogen transmission has increased interest in alternative approaches, including RNA interference to abate expression of genes essential for psyllid-mediated Ca. Liberibacter transmission. To identify genes with significantly altered expression at different life stages and conditions of CLso/CLas infection, cDNA libraries were constructed for CLso-infected and -uninfected PoP adults and nymphal instars. Illumina sequencing produced 199,081,451 reads that were assembled into 82,224 unique transcripts. PoP and the analogous transcripts from ACP adult and nymphs reported elsewhere were annotated, organized into functional gene groups using the Gene Ontology classification system, and analyzed for differential in silico expression. Expression profiles revealed vector life stage differences and differential gene expression associated with Liberibacter infection of the psyllid host, including invasion, immune system modulation, nutrition, and development. |
topic |
circulative-propagative transmission fastidious plant bacteria psyllid vector transcriptome |
url |
http://www.mdpi.com/2076-0817/3/4/875 |
work_keys_str_mv |
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