Generation of ORIONi001-A induced pluripotent stem cell line for in vitro modeling of sporadic form of amyotrophic lateral sclerosis

We generated new in vitro model for sporadic form of amyotrophic lateral sclerosis by reprogramming isolated skin fibroblasts into iPSCs. Fibroblasts were reprogrammed with commercially available synthetic polycistronic, self-replicating RNA vector. As verified by FISH, an early passages of a new iP...

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Main Authors: Jan Strnadel, Romana Zahumenska, Vladimir Nosal, Marek Smolar, Juraj Marcinek, Michal Kalman, Stefan Juhas, Jana Juhasova, Hana Studenovska, Hugo Dumortier, Tomas Chromec, Henrieta Skovierova, Barbora Mitruskova, Ivan Kapralik, Sandra Mersakova, Dusan Brany, Erika Halasova
Format: Article
Language:English
Published: Elsevier 2020-10-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506120302828
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spelling doaj-361babd4d2894313b434132a9c9db3352020-11-25T03:59:51ZengElsevierStem Cell Research1873-50612020-10-0148101981Generation of ORIONi001-A induced pluripotent stem cell line for in vitro modeling of sporadic form of amyotrophic lateral sclerosisJan Strnadel0Romana Zahumenska1Vladimir Nosal2Marek Smolar3Juraj Marcinek4Michal Kalman5Stefan Juhas6Jana Juhasova7Hana Studenovska8Hugo Dumortier9Tomas Chromec10Henrieta Skovierova11Barbora Mitruskova12Ivan Kapralik13Sandra Mersakova14Dusan Brany15Erika Halasova16Biomedical Centre Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 4D, 036 01 Martin, Slovakia; Corresponding author.Biomedical Centre Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 4D, 036 01 Martin, SlovakiaClinic of Neurology, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, University Hospital, 036 01 Martin, SlovakiaClinic of Surgery and Transplant Center, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, University Hospital, 036 01 Martin, SlovakiaDepartment of Pathological Anatomy, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, University Hospital, 036 01 Martin, SlovakiaDepartment of Pathological Anatomy, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, University Hospital, 036 01 Martin, SlovakiaPIGMOD Centre, Institute of Animal Physiology and Genetics, Czech Academy of Sciences, v.v.i., 277 21 Liběchov, Czech RepublicPIGMOD Centre, Institute of Animal Physiology and Genetics, Czech Academy of Sciences, v.v.i., 277 21 Liběchov, Czech RepublicDepartment of Biomaterials and Bioanalogous Systems Institute of Macromolecular Chemistry, Czech Academy of Sciences, 162 06 Prague, Czech RepublicBiomedical Centre Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 4D, 036 01 Martin, SlovakiaBiomedical Centre Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 4D, 036 01 Martin, SlovakiaBiomedical Centre Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 4D, 036 01 Martin, SlovakiaDepartment of Clinical Genetics, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, University Hospital, 036 01 Martin, SlovakiaDepartment of Clinical Genetics, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, University Hospital, 036 01 Martin, SlovakiaBiomedical Centre Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 4D, 036 01 Martin, SlovakiaBiomedical Centre Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 4D, 036 01 Martin, SlovakiaBiomedical Centre Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 4D, 036 01 Martin, SlovakiaWe generated new in vitro model for sporadic form of amyotrophic lateral sclerosis by reprogramming isolated skin fibroblasts into iPSCs. Fibroblasts were reprogrammed with commercially available synthetic polycistronic, self-replicating RNA vector. As verified by FISH, an early passages of a new iPSC line showed mosaic karyotype (cells with normal and abnormal karyotype 46,XY,t(2;14)(q13;p12) were present), while late passages contained only cells with abnormal karyotype. New iPSCs differentiated into all three germ layers and formed a teratoma in nude mice. Our iPSC line represents a new model for therapy testing and drug development in the field of ALS research.http://www.sciencedirect.com/science/article/pii/S1873506120302828
collection DOAJ
language English
format Article
sources DOAJ
author Jan Strnadel
Romana Zahumenska
Vladimir Nosal
Marek Smolar
Juraj Marcinek
Michal Kalman
Stefan Juhas
Jana Juhasova
Hana Studenovska
Hugo Dumortier
Tomas Chromec
Henrieta Skovierova
Barbora Mitruskova
Ivan Kapralik
Sandra Mersakova
Dusan Brany
Erika Halasova
spellingShingle Jan Strnadel
Romana Zahumenska
Vladimir Nosal
Marek Smolar
Juraj Marcinek
Michal Kalman
Stefan Juhas
Jana Juhasova
Hana Studenovska
Hugo Dumortier
Tomas Chromec
Henrieta Skovierova
Barbora Mitruskova
Ivan Kapralik
Sandra Mersakova
Dusan Brany
Erika Halasova
Generation of ORIONi001-A induced pluripotent stem cell line for in vitro modeling of sporadic form of amyotrophic lateral sclerosis
Stem Cell Research
author_facet Jan Strnadel
Romana Zahumenska
Vladimir Nosal
Marek Smolar
Juraj Marcinek
Michal Kalman
Stefan Juhas
Jana Juhasova
Hana Studenovska
Hugo Dumortier
Tomas Chromec
Henrieta Skovierova
Barbora Mitruskova
Ivan Kapralik
Sandra Mersakova
Dusan Brany
Erika Halasova
author_sort Jan Strnadel
title Generation of ORIONi001-A induced pluripotent stem cell line for in vitro modeling of sporadic form of amyotrophic lateral sclerosis
title_short Generation of ORIONi001-A induced pluripotent stem cell line for in vitro modeling of sporadic form of amyotrophic lateral sclerosis
title_full Generation of ORIONi001-A induced pluripotent stem cell line for in vitro modeling of sporadic form of amyotrophic lateral sclerosis
title_fullStr Generation of ORIONi001-A induced pluripotent stem cell line for in vitro modeling of sporadic form of amyotrophic lateral sclerosis
title_full_unstemmed Generation of ORIONi001-A induced pluripotent stem cell line for in vitro modeling of sporadic form of amyotrophic lateral sclerosis
title_sort generation of orioni001-a induced pluripotent stem cell line for in vitro modeling of sporadic form of amyotrophic lateral sclerosis
publisher Elsevier
series Stem Cell Research
issn 1873-5061
publishDate 2020-10-01
description We generated new in vitro model for sporadic form of amyotrophic lateral sclerosis by reprogramming isolated skin fibroblasts into iPSCs. Fibroblasts were reprogrammed with commercially available synthetic polycistronic, self-replicating RNA vector. As verified by FISH, an early passages of a new iPSC line showed mosaic karyotype (cells with normal and abnormal karyotype 46,XY,t(2;14)(q13;p12) were present), while late passages contained only cells with abnormal karyotype. New iPSCs differentiated into all three germ layers and formed a teratoma in nude mice. Our iPSC line represents a new model for therapy testing and drug development in the field of ALS research.
url http://www.sciencedirect.com/science/article/pii/S1873506120302828
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