Generation of ORIONi001-A induced pluripotent stem cell line for in vitro modeling of sporadic form of amyotrophic lateral sclerosis
We generated new in vitro model for sporadic form of amyotrophic lateral sclerosis by reprogramming isolated skin fibroblasts into iPSCs. Fibroblasts were reprogrammed with commercially available synthetic polycistronic, self-replicating RNA vector. As verified by FISH, an early passages of a new iP...
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doaj-361babd4d2894313b434132a9c9db3352020-11-25T03:59:51ZengElsevierStem Cell Research1873-50612020-10-0148101981Generation of ORIONi001-A induced pluripotent stem cell line for in vitro modeling of sporadic form of amyotrophic lateral sclerosisJan Strnadel0Romana Zahumenska1Vladimir Nosal2Marek Smolar3Juraj Marcinek4Michal Kalman5Stefan Juhas6Jana Juhasova7Hana Studenovska8Hugo Dumortier9Tomas Chromec10Henrieta Skovierova11Barbora Mitruskova12Ivan Kapralik13Sandra Mersakova14Dusan Brany15Erika Halasova16Biomedical Centre Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 4D, 036 01 Martin, Slovakia; Corresponding author.Biomedical Centre Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 4D, 036 01 Martin, SlovakiaClinic of Neurology, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, University Hospital, 036 01 Martin, SlovakiaClinic of Surgery and Transplant Center, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, University Hospital, 036 01 Martin, SlovakiaDepartment of Pathological Anatomy, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, University Hospital, 036 01 Martin, SlovakiaDepartment of Pathological Anatomy, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, University Hospital, 036 01 Martin, SlovakiaPIGMOD Centre, Institute of Animal Physiology and Genetics, Czech Academy of Sciences, v.v.i., 277 21 Liběchov, Czech RepublicPIGMOD Centre, Institute of Animal Physiology and Genetics, Czech Academy of Sciences, v.v.i., 277 21 Liběchov, Czech RepublicDepartment of Biomaterials and Bioanalogous Systems Institute of Macromolecular Chemistry, Czech Academy of Sciences, 162 06 Prague, Czech RepublicBiomedical Centre Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 4D, 036 01 Martin, SlovakiaBiomedical Centre Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 4D, 036 01 Martin, SlovakiaBiomedical Centre Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 4D, 036 01 Martin, SlovakiaDepartment of Clinical Genetics, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, University Hospital, 036 01 Martin, SlovakiaDepartment of Clinical Genetics, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, University Hospital, 036 01 Martin, SlovakiaBiomedical Centre Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 4D, 036 01 Martin, SlovakiaBiomedical Centre Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 4D, 036 01 Martin, SlovakiaBiomedical Centre Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 4D, 036 01 Martin, SlovakiaWe generated new in vitro model for sporadic form of amyotrophic lateral sclerosis by reprogramming isolated skin fibroblasts into iPSCs. Fibroblasts were reprogrammed with commercially available synthetic polycistronic, self-replicating RNA vector. As verified by FISH, an early passages of a new iPSC line showed mosaic karyotype (cells with normal and abnormal karyotype 46,XY,t(2;14)(q13;p12) were present), while late passages contained only cells with abnormal karyotype. New iPSCs differentiated into all three germ layers and formed a teratoma in nude mice. Our iPSC line represents a new model for therapy testing and drug development in the field of ALS research.http://www.sciencedirect.com/science/article/pii/S1873506120302828 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jan Strnadel Romana Zahumenska Vladimir Nosal Marek Smolar Juraj Marcinek Michal Kalman Stefan Juhas Jana Juhasova Hana Studenovska Hugo Dumortier Tomas Chromec Henrieta Skovierova Barbora Mitruskova Ivan Kapralik Sandra Mersakova Dusan Brany Erika Halasova |
spellingShingle |
Jan Strnadel Romana Zahumenska Vladimir Nosal Marek Smolar Juraj Marcinek Michal Kalman Stefan Juhas Jana Juhasova Hana Studenovska Hugo Dumortier Tomas Chromec Henrieta Skovierova Barbora Mitruskova Ivan Kapralik Sandra Mersakova Dusan Brany Erika Halasova Generation of ORIONi001-A induced pluripotent stem cell line for in vitro modeling of sporadic form of amyotrophic lateral sclerosis Stem Cell Research |
author_facet |
Jan Strnadel Romana Zahumenska Vladimir Nosal Marek Smolar Juraj Marcinek Michal Kalman Stefan Juhas Jana Juhasova Hana Studenovska Hugo Dumortier Tomas Chromec Henrieta Skovierova Barbora Mitruskova Ivan Kapralik Sandra Mersakova Dusan Brany Erika Halasova |
author_sort |
Jan Strnadel |
title |
Generation of ORIONi001-A induced pluripotent stem cell line for in vitro modeling of sporadic form of amyotrophic lateral sclerosis |
title_short |
Generation of ORIONi001-A induced pluripotent stem cell line for in vitro modeling of sporadic form of amyotrophic lateral sclerosis |
title_full |
Generation of ORIONi001-A induced pluripotent stem cell line for in vitro modeling of sporadic form of amyotrophic lateral sclerosis |
title_fullStr |
Generation of ORIONi001-A induced pluripotent stem cell line for in vitro modeling of sporadic form of amyotrophic lateral sclerosis |
title_full_unstemmed |
Generation of ORIONi001-A induced pluripotent stem cell line for in vitro modeling of sporadic form of amyotrophic lateral sclerosis |
title_sort |
generation of orioni001-a induced pluripotent stem cell line for in vitro modeling of sporadic form of amyotrophic lateral sclerosis |
publisher |
Elsevier |
series |
Stem Cell Research |
issn |
1873-5061 |
publishDate |
2020-10-01 |
description |
We generated new in vitro model for sporadic form of amyotrophic lateral sclerosis by reprogramming isolated skin fibroblasts into iPSCs. Fibroblasts were reprogrammed with commercially available synthetic polycistronic, self-replicating RNA vector. As verified by FISH, an early passages of a new iPSC line showed mosaic karyotype (cells with normal and abnormal karyotype 46,XY,t(2;14)(q13;p12) were present), while late passages contained only cells with abnormal karyotype. New iPSCs differentiated into all three germ layers and formed a teratoma in nude mice. Our iPSC line represents a new model for therapy testing and drug development in the field of ALS research. |
url |
http://www.sciencedirect.com/science/article/pii/S1873506120302828 |
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