Polydatin Protects Bone Marrow Stem Cells against Oxidative Injury: Involvement of Nrf 2/ARE Pathways
Polydatin, a glucoside of resveratrol, has been reported to possess potent antioxidative effects. In the present study, we aimed to investigate the effects of polydatin in bone marrow-derived mesenchymal stem cells (BMSCs) death caused by hydrogen peroxide (H2O2), imitating the microenvironment surr...
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Online Access: | http://dx.doi.org/10.1155/2016/9394150 |
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doaj-3638b1094c5448ed805be35662d53d732020-11-24T22:49:54ZengHindawi LimitedStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/93941509394150Polydatin Protects Bone Marrow Stem Cells against Oxidative Injury: Involvement of Nrf 2/ARE PathwaysMeihui Chen0Yu Hou1Dingkun Lin2Research Laboratory of Spine Degenerative Disease, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510120, ChinaResearch Laboratory of Spine Degenerative Disease, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510120, ChinaResearch Laboratory of Spine Degenerative Disease, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510120, ChinaPolydatin, a glucoside of resveratrol, has been reported to possess potent antioxidative effects. In the present study, we aimed to investigate the effects of polydatin in bone marrow-derived mesenchymal stem cells (BMSCs) death caused by hydrogen peroxide (H2O2), imitating the microenvironment surrounding transplanted cells in the injured spinal cord in vitro. In our study, MTT results showed that polydatin effectively prevented the decrease of cell viability caused by H2O2. Hochest 33258, Annexin V-PI, and Western blot assay showed H2O2-induced apoptosis in BMSCs, which was attenuated by polydatin. Further studies indicated that polydatin significantly protects BMSCs against apoptosis due to its antioxidative effects and the regulation of Nrf 2/ARE pathway. Taken together, our results indicate that polydatin could be used in combination with BMSCs for the treatment of spinal cord injury by improving the cell survival and oxidative stress microenvironments.http://dx.doi.org/10.1155/2016/9394150 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Meihui Chen Yu Hou Dingkun Lin |
spellingShingle |
Meihui Chen Yu Hou Dingkun Lin Polydatin Protects Bone Marrow Stem Cells against Oxidative Injury: Involvement of Nrf 2/ARE Pathways Stem Cells International |
author_facet |
Meihui Chen Yu Hou Dingkun Lin |
author_sort |
Meihui Chen |
title |
Polydatin Protects Bone Marrow Stem Cells against Oxidative Injury: Involvement of Nrf 2/ARE Pathways |
title_short |
Polydatin Protects Bone Marrow Stem Cells against Oxidative Injury: Involvement of Nrf 2/ARE Pathways |
title_full |
Polydatin Protects Bone Marrow Stem Cells against Oxidative Injury: Involvement of Nrf 2/ARE Pathways |
title_fullStr |
Polydatin Protects Bone Marrow Stem Cells against Oxidative Injury: Involvement of Nrf 2/ARE Pathways |
title_full_unstemmed |
Polydatin Protects Bone Marrow Stem Cells against Oxidative Injury: Involvement of Nrf 2/ARE Pathways |
title_sort |
polydatin protects bone marrow stem cells against oxidative injury: involvement of nrf 2/are pathways |
publisher |
Hindawi Limited |
series |
Stem Cells International |
issn |
1687-966X 1687-9678 |
publishDate |
2016-01-01 |
description |
Polydatin, a glucoside of resveratrol, has been reported to possess potent antioxidative effects. In the present study, we aimed to investigate the effects of polydatin in bone marrow-derived mesenchymal stem cells (BMSCs) death caused by hydrogen peroxide (H2O2), imitating the microenvironment surrounding transplanted cells in the injured spinal cord in vitro. In our study, MTT results showed that polydatin effectively prevented the decrease of cell viability caused by H2O2. Hochest 33258, Annexin V-PI, and Western blot assay showed H2O2-induced apoptosis in BMSCs, which was attenuated by polydatin. Further studies indicated that polydatin significantly protects BMSCs against apoptosis due to its antioxidative effects and the regulation of Nrf 2/ARE pathway. Taken together, our results indicate that polydatin could be used in combination with BMSCs for the treatment of spinal cord injury by improving the cell survival and oxidative stress microenvironments. |
url |
http://dx.doi.org/10.1155/2016/9394150 |
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