Altered distribution of platelet-activating factor-acetylhydrolase activity between LDL and HDL as a function of the severity of hypercholesterolemia
Platelet-activating factor-acetylhydrolase (PAF-AH) is a lipoprotein-associated phospholipase A2 capable of hydrolyzing platelet-activating factor (PAF) and oxidatively modified phospholipids. We studied the plasma- and lipoprotein-associated PAF-AH activity in patients with primary hypercholesterol...
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doaj-365f59849a924c4584b92aa7345135e42021-04-27T04:38:19ZengElsevierJournal of Lipid Research0022-22752002-02-01432256263Altered distribution of platelet-activating factor-acetylhydrolase activity between LDL and HDL as a function of the severity of hypercholesterolemiaVasilis Tsimihodimos0Sonia-Athena P. Karabina1Afroditi P. Tambaki2Eleni Bairaktari3George Miltiadous4John A. Goudevenos5Marios A. Cariolou6M. John Chapman7Alexandros D. Tselepis8Moses Elisaf9Department of Internal Medicine, University of Ioannina, 45110 Ioannina, GreeceLaboratory of Biochemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, GreeceLaboratory of Biochemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, GreeceLaboratory of Biological Chemistry, Medical School, University of Ioannina, 45110 Ioannina, GreeceDepartment of Internal Medicine, University of Ioannina, 45110 Ioannina, GreeceDepartment of Cardiology, University of Ioannina, 45110 Ioannina, GreeceMolecular Genetics Unit B-DNA Identification Laboratory, The Cyprus Institute of Neurology and Genetics, Nicosia, CyprusInstitut National de la Santé et de la Recherché, Unité 551, Hôpital de la Pitié, 83 Bd de l'Hôpital, 75651 Paris Cedex 13, FranceTo whom correspondence should be addressed.; Laboratory of Biochemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, GreeceDepartment of Internal Medicine, University of Ioannina, 45110 Ioannina, GreecePlatelet-activating factor-acetylhydrolase (PAF-AH) is a lipoprotein-associated phospholipase A2 capable of hydrolyzing platelet-activating factor (PAF) and oxidatively modified phospholipids. We studied the plasma- and lipoprotein-associated PAF-AH activity in patients with primary hypercholesterolemia. Thirty-eight unrelated patients with heterozygous familial hypercholesterolemia (HeteroFH), five patients with homozygous FH (HomoFH), and 33 patients with primary non-FH hypercholesterolemia (NonFH) participated in the study. In all patient groups the plasma PAF-AH activity was significantly elevated compared with 33 normolipidemic controls, the HomoFH having the highest and the NonFH patients showing the lowest enzyme activity. Gradient ultracentrifugation studies showed that this increase is not only due to the elevation in the plasma LDL but also to the increase in the PAF-AH activity associated with each LDL subfraction, being more profound in the small-dense LDL-5. Unlike LDL, no difference in the HDL-associated PAF-AH activity was observed among all groups. Consequently, an altered distribution of enzyme activity among apolipoprotein B (apoB)- and apolipoprotein A-I (apoA-I)-containing lipoproteins is observed in hypercholesterolemic patients, resulting in a significant decrease in the ratio of the HDL-associated PAF-AH to the total plasma enzyme activity compared with controls. This reduction is proportional to the increase of the plasma LDL-cholesterol (LDL-C) levels and consequently to the severity of the hypercholesterolemia. Thus, the ratio of HDL-associated PAF-AH-total plasma enzyme activity may be useful as a potential marker of atherogenicity in subjects with primary hypercholesterolemia.—Tsimihodimos, V., S-A. P. Karabina, A. P. Tambaki, E. Bairaktari, G. Miltiadous, J. A. Goudevenos, M. A. Cariolou, M. J. Chapman, A. D. Tselepis, and M. Elisaf. Altered distribution of platelet-activating factor-acetylhydrolase activity between LDL and HDL as a function of the severity of hypercholesterolemia.http://www.sciencedirect.com/science/article/pii/S002222752030167Xfamilial hypercholesterolemialow density lipoproteinlipoprotein subspecieshigh density lipoproteinPAF-acetylhydrolasepolygenic hypercholesterolemia |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Vasilis Tsimihodimos Sonia-Athena P. Karabina Afroditi P. Tambaki Eleni Bairaktari George Miltiadous John A. Goudevenos Marios A. Cariolou M. John Chapman Alexandros D. Tselepis Moses Elisaf |
spellingShingle |
Vasilis Tsimihodimos Sonia-Athena P. Karabina Afroditi P. Tambaki Eleni Bairaktari George Miltiadous John A. Goudevenos Marios A. Cariolou M. John Chapman Alexandros D. Tselepis Moses Elisaf Altered distribution of platelet-activating factor-acetylhydrolase activity between LDL and HDL as a function of the severity of hypercholesterolemia Journal of Lipid Research familial hypercholesterolemia low density lipoprotein lipoprotein subspecies high density lipoprotein PAF-acetylhydrolase polygenic hypercholesterolemia |
author_facet |
Vasilis Tsimihodimos Sonia-Athena P. Karabina Afroditi P. Tambaki Eleni Bairaktari George Miltiadous John A. Goudevenos Marios A. Cariolou M. John Chapman Alexandros D. Tselepis Moses Elisaf |
author_sort |
Vasilis Tsimihodimos |
title |
Altered distribution of platelet-activating factor-acetylhydrolase activity between LDL and HDL as a function of the severity of hypercholesterolemia |
title_short |
Altered distribution of platelet-activating factor-acetylhydrolase activity between LDL and HDL as a function of the severity of hypercholesterolemia |
title_full |
Altered distribution of platelet-activating factor-acetylhydrolase activity between LDL and HDL as a function of the severity of hypercholesterolemia |
title_fullStr |
Altered distribution of platelet-activating factor-acetylhydrolase activity between LDL and HDL as a function of the severity of hypercholesterolemia |
title_full_unstemmed |
Altered distribution of platelet-activating factor-acetylhydrolase activity between LDL and HDL as a function of the severity of hypercholesterolemia |
title_sort |
altered distribution of platelet-activating factor-acetylhydrolase activity between ldl and hdl as a function of the severity of hypercholesterolemia |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
2002-02-01 |
description |
Platelet-activating factor-acetylhydrolase (PAF-AH) is a lipoprotein-associated phospholipase A2 capable of hydrolyzing platelet-activating factor (PAF) and oxidatively modified phospholipids. We studied the plasma- and lipoprotein-associated PAF-AH activity in patients with primary hypercholesterolemia. Thirty-eight unrelated patients with heterozygous familial hypercholesterolemia (HeteroFH), five patients with homozygous FH (HomoFH), and 33 patients with primary non-FH hypercholesterolemia (NonFH) participated in the study. In all patient groups the plasma PAF-AH activity was significantly elevated compared with 33 normolipidemic controls, the HomoFH having the highest and the NonFH patients showing the lowest enzyme activity. Gradient ultracentrifugation studies showed that this increase is not only due to the elevation in the plasma LDL but also to the increase in the PAF-AH activity associated with each LDL subfraction, being more profound in the small-dense LDL-5. Unlike LDL, no difference in the HDL-associated PAF-AH activity was observed among all groups. Consequently, an altered distribution of enzyme activity among apolipoprotein B (apoB)- and apolipoprotein A-I (apoA-I)-containing lipoproteins is observed in hypercholesterolemic patients, resulting in a significant decrease in the ratio of the HDL-associated PAF-AH to the total plasma enzyme activity compared with controls. This reduction is proportional to the increase of the plasma LDL-cholesterol (LDL-C) levels and consequently to the severity of the hypercholesterolemia. Thus, the ratio of HDL-associated PAF-AH-total plasma enzyme activity may be useful as a potential marker of atherogenicity in subjects with primary hypercholesterolemia.—Tsimihodimos, V., S-A. P. Karabina, A. P. Tambaki, E. Bairaktari, G. Miltiadous, J. A. Goudevenos, M. A. Cariolou, M. J. Chapman, A. D. Tselepis, and M. Elisaf. Altered distribution of platelet-activating factor-acetylhydrolase activity between LDL and HDL as a function of the severity of hypercholesterolemia. |
topic |
familial hypercholesterolemia low density lipoprotein lipoprotein subspecies high density lipoprotein PAF-acetylhydrolase polygenic hypercholesterolemia |
url |
http://www.sciencedirect.com/science/article/pii/S002222752030167X |
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