Increased vimentin mRNA expression in MCF-7 breast cancer cell line after repeated endoxifen-treatment
Background: Epithelial mesenchymal transition (EMT) plays a significant role in the development of cancer cell resistance to drugs. Vimentin, a type III intermediate filament protein, is a marker of EMT. Vimentin's over-expression in cancer correlates well with increased tumor growth, change i...
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doaj-366dafed8dc84c2fbb68570e7c5783222020-11-25T02:39:24ZengFaculty of Medicine Universitas Indonesia Medical Journal of Indonesia0853-17732252-80832017-01-0125410.13181/mji.v25i4.13971141Increased vimentin mRNA expression in MCF-7 breast cancer cell line after repeated endoxifen-treatmentParamita Paramita0Melva Louisa1Nafrialdi Nafrialdi2Faculty of Medicine, Universitas Indonesia, JakartaDepartement of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Indonesia, JakartaDepartement of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Indonesia, Jakarta Background: Epithelial mesenchymal transition (EMT) plays a significant role in the development of cancer cell resistance to drugs. Vimentin, a type III intermediate filament protein, is a marker of EMT. Vimentin's over-expression in cancer correlates well with increased tumor growth, change in cell shape and poor prognosis. Endoxifen is an active metabolite of tamoxifen and has become a new potent agent in the treatment of breast cancer. This is a study that aimed to investigate the effect of endoxifen exposure with or without estradiol on cell viability, cell morphology and EMT progression through the analysis of vimentin mRNA expression after 4-week treatment. Methods: Endoxifen, 100 nM or 1,000 nM, with or without beta-estradiol were given repeatedly to MCF-7 cells. Cells treated with dimethyl sulfoxide (DMSO) 0.001% were used as control. After 2- and 4-week exposure, the cells were counted, analyzed for mRNA vimentin expression, and observed for morphological changes. Results: Compared to control, there were significant decreases in vimentin mRNA expressions in endoxifen and endoxifen+β-estradiol treated cells after 2-weeks, which then significantly increased after 4-week compared with the 2-week exposure. We found no change in morphology of MCF-7 cells. Conclusion: Repeated exposure of endoxifen might induce EMT progression through increased expression of vimentin in MCF-7 breast cancer cell line. http://mji.ui.ac.id/journal/index.php/mji/article/view/1397endoxifenEMTvimentin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Paramita Paramita Melva Louisa Nafrialdi Nafrialdi |
spellingShingle |
Paramita Paramita Melva Louisa Nafrialdi Nafrialdi Increased vimentin mRNA expression in MCF-7 breast cancer cell line after repeated endoxifen-treatment Medical Journal of Indonesia endoxifen EMT vimentin |
author_facet |
Paramita Paramita Melva Louisa Nafrialdi Nafrialdi |
author_sort |
Paramita Paramita |
title |
Increased vimentin mRNA expression in MCF-7 breast cancer cell line after repeated endoxifen-treatment |
title_short |
Increased vimentin mRNA expression in MCF-7 breast cancer cell line after repeated endoxifen-treatment |
title_full |
Increased vimentin mRNA expression in MCF-7 breast cancer cell line after repeated endoxifen-treatment |
title_fullStr |
Increased vimentin mRNA expression in MCF-7 breast cancer cell line after repeated endoxifen-treatment |
title_full_unstemmed |
Increased vimentin mRNA expression in MCF-7 breast cancer cell line after repeated endoxifen-treatment |
title_sort |
increased vimentin mrna expression in mcf-7 breast cancer cell line after repeated endoxifen-treatment |
publisher |
Faculty of Medicine Universitas Indonesia |
series |
Medical Journal of Indonesia |
issn |
0853-1773 2252-8083 |
publishDate |
2017-01-01 |
description |
Background: Epithelial mesenchymal transition (EMT) plays a significant role in the development of cancer cell resistance to drugs. Vimentin, a type III intermediate filament protein, is a marker of EMT. Vimentin's over-expression in cancer correlates well with increased tumor growth, change in cell shape and poor prognosis. Endoxifen is an active metabolite of tamoxifen and has become a new potent agent in the treatment of breast cancer. This is a study that aimed to investigate the effect of endoxifen exposure with or without estradiol on cell viability, cell morphology and EMT progression through the analysis of vimentin mRNA expression after 4-week treatment.
Methods: Endoxifen, 100 nM or 1,000 nM, with or without beta-estradiol were given repeatedly to MCF-7 cells. Cells treated with dimethyl sulfoxide (DMSO) 0.001% were used as control. After 2- and 4-week exposure, the cells were counted, analyzed for mRNA vimentin expression, and observed for morphological changes.
Results: Compared to control, there were significant decreases in vimentin mRNA expressions in endoxifen and endoxifen+β-estradiol treated cells after 2-weeks, which then significantly increased after 4-week compared with the 2-week exposure. We found no change in morphology of MCF-7 cells.
Conclusion: Repeated exposure of endoxifen might induce EMT progression through increased expression of vimentin in MCF-7 breast cancer cell line.
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topic |
endoxifen EMT vimentin |
url |
http://mji.ui.ac.id/journal/index.php/mji/article/view/1397 |
work_keys_str_mv |
AT paramitaparamita increasedvimentinmrnaexpressioninmcf7breastcancercelllineafterrepeatedendoxifentreatment AT melvalouisa increasedvimentinmrnaexpressioninmcf7breastcancercelllineafterrepeatedendoxifentreatment AT nafrialdinafrialdi increasedvimentinmrnaexpressioninmcf7breastcancercelllineafterrepeatedendoxifentreatment |
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1724786382298152960 |