Identification and Extraction Optimization of Active Constituents in <i>Citrus junos</i> Seib ex TANAKA Peel and Its Biological Evaluation
<i>Citrus junos</i> Seib ex TANAKA possesses various biological effects. It has been used in oriental remedies for blood circulation and the common cold. Recently, biological effects of <i>C. junos</i> peel have been reported. However, optimization of the biological propertie...
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doaj-36adbd33e05e49c48123d6d8c6f2559e2020-11-25T01:13:40ZengMDPI AGMolecules1420-30492019-02-0124468010.3390/molecules24040680molecules24040680Identification and Extraction Optimization of Active Constituents in <i>Citrus junos</i> Seib ex TANAKA Peel and Its Biological EvaluationJung-hyun Shim0Jung-il Chae1Seung-sik Cho2Department of Pharmacy, College of Pharmacy, Mokpo National University, Muan, Jeonnam 58554, KoreaDepartment of Dental Pharmacology, School of Dentistry and Institute of Oral Bioscience, BK21 Plus, Chonbuk National University, Jeonju 56443, KoreaDepartment of Pharmacy, College of Pharmacy, Mokpo National University, Muan, Jeonnam 58554, Korea<i>Citrus junos</i> Seib ex TANAKA possesses various biological effects. It has been used in oriental remedies for blood circulation and the common cold. Recently, biological effects of <i>C. junos</i> peel have been reported. However, optimization of the biological properties of <i>C. junos</i> peel preparations has yet to be reported on. We developed a high-performance liquid chromatography (HPLC) method for quantification of the active constituents in <i>C. junos</i> peel. Hot water and ethanolic extracts of <i>C. junos</i> peel were prepared and their chemical profiles and biological activities were evaluated. The 80% ethanolic extract demonstrated the greatest antioxidant activity and phenolic content, while the 100% ethanolic extract had the greatest xanthine oxidase inhibitory activity. Elastase inhibition activity was superior in aqueous and 20% ethanolic extracts. The contents of two flavonoids were highest in the 100% ethanolic extract. We postulated that the antioxidant and anti-aging effects of <i>C. junos</i> peel extract could be attributed to phenolics such as flavonoids. Our results suggest that the flavonoid-rich extract of <i>C. junos</i> may be utilized for the treatment and prevention of metabolic disease and hyperuricemia while the water-soluble extract of <i>C. junos</i> could be used as a source for its anti-aging properties.https://www.mdpi.com/1420-3049/24/4/680<i>Citrus junos</i> Seib ex TANAKAantioxidantxanthine oxidaseelastase |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jung-hyun Shim Jung-il Chae Seung-sik Cho |
spellingShingle |
Jung-hyun Shim Jung-il Chae Seung-sik Cho Identification and Extraction Optimization of Active Constituents in <i>Citrus junos</i> Seib ex TANAKA Peel and Its Biological Evaluation Molecules <i>Citrus junos</i> Seib ex TANAKA antioxidant xanthine oxidase elastase |
author_facet |
Jung-hyun Shim Jung-il Chae Seung-sik Cho |
author_sort |
Jung-hyun Shim |
title |
Identification and Extraction Optimization of Active Constituents in <i>Citrus junos</i> Seib ex TANAKA Peel and Its Biological Evaluation |
title_short |
Identification and Extraction Optimization of Active Constituents in <i>Citrus junos</i> Seib ex TANAKA Peel and Its Biological Evaluation |
title_full |
Identification and Extraction Optimization of Active Constituents in <i>Citrus junos</i> Seib ex TANAKA Peel and Its Biological Evaluation |
title_fullStr |
Identification and Extraction Optimization of Active Constituents in <i>Citrus junos</i> Seib ex TANAKA Peel and Its Biological Evaluation |
title_full_unstemmed |
Identification and Extraction Optimization of Active Constituents in <i>Citrus junos</i> Seib ex TANAKA Peel and Its Biological Evaluation |
title_sort |
identification and extraction optimization of active constituents in <i>citrus junos</i> seib ex tanaka peel and its biological evaluation |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2019-02-01 |
description |
<i>Citrus junos</i> Seib ex TANAKA possesses various biological effects. It has been used in oriental remedies for blood circulation and the common cold. Recently, biological effects of <i>C. junos</i> peel have been reported. However, optimization of the biological properties of <i>C. junos</i> peel preparations has yet to be reported on. We developed a high-performance liquid chromatography (HPLC) method for quantification of the active constituents in <i>C. junos</i> peel. Hot water and ethanolic extracts of <i>C. junos</i> peel were prepared and their chemical profiles and biological activities were evaluated. The 80% ethanolic extract demonstrated the greatest antioxidant activity and phenolic content, while the 100% ethanolic extract had the greatest xanthine oxidase inhibitory activity. Elastase inhibition activity was superior in aqueous and 20% ethanolic extracts. The contents of two flavonoids were highest in the 100% ethanolic extract. We postulated that the antioxidant and anti-aging effects of <i>C. junos</i> peel extract could be attributed to phenolics such as flavonoids. Our results suggest that the flavonoid-rich extract of <i>C. junos</i> may be utilized for the treatment and prevention of metabolic disease and hyperuricemia while the water-soluble extract of <i>C. junos</i> could be used as a source for its anti-aging properties. |
topic |
<i>Citrus junos</i> Seib ex TANAKA antioxidant xanthine oxidase elastase |
url |
https://www.mdpi.com/1420-3049/24/4/680 |
work_keys_str_mv |
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