Repetitive element-mediated recombination as a mechanism for new gene origination in Drosophila.
Previous studies of repetitive elements (REs) have implicated a mechanistic role in generating new chimerical genes. Such examples are consistent with the classic model for exon shuffling, which relies on non-homologous recombination. However, recent data for chromosomal aberrations in model organis...
Main Authors: | , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2008-01-01
|
Series: | PLoS Genetics |
Online Access: | http://europepmc.org/articles/PMC2211543?pdf=render |
id |
doaj-36b65e8e6c304ba29c7515266c495cd6 |
---|---|
record_format |
Article |
spelling |
doaj-36b65e8e6c304ba29c7515266c495cd62020-11-25T02:49:24ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042008-01-0141e310.1371/journal.pgen.0040003Repetitive element-mediated recombination as a mechanism for new gene origination in Drosophila.Shuang YangJ Roman ArguelloXin LiYun DingQi ZhouYing ChenYue ZhangRuoping ZhaoFrédéric BrunetLixin PengManyuan LongWen WangPrevious studies of repetitive elements (REs) have implicated a mechanistic role in generating new chimerical genes. Such examples are consistent with the classic model for exon shuffling, which relies on non-homologous recombination. However, recent data for chromosomal aberrations in model organisms suggest that ectopic homology-dependent recombination may also be important. Lack of a dataset comprising experimentally verified young duplicates has hampered an effective examination of these models as well as an investigation of sequence features that mediate the rearrangements. Here we use approximately 7,000 cDNA probes (approximately 112,000 primary images) to screen eight species within the Drosophila melanogaster subgroup and identify 17 duplicates that were generated through ectopic recombination within the last 12 mys. Most of these are functional and have evolved divergent expression patterns and novel chimeric structures. Examination of their flanking sequences revealed an excess of repetitive sequences, with the majority belonging to the transposable element DNAREP1 family, associated with the new genes. Our dataset strongly suggests an important role for REs in the generation of chimeric genes within these species.http://europepmc.org/articles/PMC2211543?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shuang Yang J Roman Arguello Xin Li Yun Ding Qi Zhou Ying Chen Yue Zhang Ruoping Zhao Frédéric Brunet Lixin Peng Manyuan Long Wen Wang |
spellingShingle |
Shuang Yang J Roman Arguello Xin Li Yun Ding Qi Zhou Ying Chen Yue Zhang Ruoping Zhao Frédéric Brunet Lixin Peng Manyuan Long Wen Wang Repetitive element-mediated recombination as a mechanism for new gene origination in Drosophila. PLoS Genetics |
author_facet |
Shuang Yang J Roman Arguello Xin Li Yun Ding Qi Zhou Ying Chen Yue Zhang Ruoping Zhao Frédéric Brunet Lixin Peng Manyuan Long Wen Wang |
author_sort |
Shuang Yang |
title |
Repetitive element-mediated recombination as a mechanism for new gene origination in Drosophila. |
title_short |
Repetitive element-mediated recombination as a mechanism for new gene origination in Drosophila. |
title_full |
Repetitive element-mediated recombination as a mechanism for new gene origination in Drosophila. |
title_fullStr |
Repetitive element-mediated recombination as a mechanism for new gene origination in Drosophila. |
title_full_unstemmed |
Repetitive element-mediated recombination as a mechanism for new gene origination in Drosophila. |
title_sort |
repetitive element-mediated recombination as a mechanism for new gene origination in drosophila. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Genetics |
issn |
1553-7390 1553-7404 |
publishDate |
2008-01-01 |
description |
Previous studies of repetitive elements (REs) have implicated a mechanistic role in generating new chimerical genes. Such examples are consistent with the classic model for exon shuffling, which relies on non-homologous recombination. However, recent data for chromosomal aberrations in model organisms suggest that ectopic homology-dependent recombination may also be important. Lack of a dataset comprising experimentally verified young duplicates has hampered an effective examination of these models as well as an investigation of sequence features that mediate the rearrangements. Here we use approximately 7,000 cDNA probes (approximately 112,000 primary images) to screen eight species within the Drosophila melanogaster subgroup and identify 17 duplicates that were generated through ectopic recombination within the last 12 mys. Most of these are functional and have evolved divergent expression patterns and novel chimeric structures. Examination of their flanking sequences revealed an excess of repetitive sequences, with the majority belonging to the transposable element DNAREP1 family, associated with the new genes. Our dataset strongly suggests an important role for REs in the generation of chimeric genes within these species. |
url |
http://europepmc.org/articles/PMC2211543?pdf=render |
work_keys_str_mv |
AT shuangyang repetitiveelementmediatedrecombinationasamechanismfornewgeneoriginationindrosophila AT jromanarguello repetitiveelementmediatedrecombinationasamechanismfornewgeneoriginationindrosophila AT xinli repetitiveelementmediatedrecombinationasamechanismfornewgeneoriginationindrosophila AT yunding repetitiveelementmediatedrecombinationasamechanismfornewgeneoriginationindrosophila AT qizhou repetitiveelementmediatedrecombinationasamechanismfornewgeneoriginationindrosophila AT yingchen repetitiveelementmediatedrecombinationasamechanismfornewgeneoriginationindrosophila AT yuezhang repetitiveelementmediatedrecombinationasamechanismfornewgeneoriginationindrosophila AT ruopingzhao repetitiveelementmediatedrecombinationasamechanismfornewgeneoriginationindrosophila AT fredericbrunet repetitiveelementmediatedrecombinationasamechanismfornewgeneoriginationindrosophila AT lixinpeng repetitiveelementmediatedrecombinationasamechanismfornewgeneoriginationindrosophila AT manyuanlong repetitiveelementmediatedrecombinationasamechanismfornewgeneoriginationindrosophila AT wenwang repetitiveelementmediatedrecombinationasamechanismfornewgeneoriginationindrosophila |
_version_ |
1724743725030047744 |