Degradation and osteogenic potential of a novel poly(lactic acid)/nano-sized β-tricalcium phosphate scaffold
Lu Cao,1,2 Ping-Guo Duan,1,2 Hui-Ren Wang,1,2 Xi-Lei Li,1,2 Feng-Lai Yuan,3 Zhong-Yong Fan,4 Su-Ming Li,5 Jian Dong1,21Department of Orthopedic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China; 2State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai, Chin...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Dove Medical Press
2012-11-01
|
Series: | International Journal of Nanomedicine |
Online Access: | http://www.dovepress.com/degradation-and-osteogenic-potential-of-a-novel-polylactic-acidnano-si-a11629 |
id |
doaj-36c67a992c044a0e9367b6abb6ad1689 |
---|---|
record_format |
Article |
spelling |
doaj-36c67a992c044a0e9367b6abb6ad16892020-11-24T21:48:04ZengDove Medical PressInternational Journal of Nanomedicine1176-91141178-20132012-11-012012default58815888Degradation and osteogenic potential of a novel poly(lactic acid)/nano-sized β-tricalcium phosphate scaffoldCao LDuan PGWang HRLi XLYuan FLFan ZYLi SMDong JLu Cao,1,2 Ping-Guo Duan,1,2 Hui-Ren Wang,1,2 Xi-Lei Li,1,2 Feng-Lai Yuan,3 Zhong-Yong Fan,4 Su-Ming Li,5 Jian Dong1,21Department of Orthopedic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China; 2State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai, China; 3Affiliated Third Hospital of Nantong University, Wuxi, Jiangsu, China; 4Department of Materials Science, Fudan University, Shanghai, China; 5Max Mousseron Institute on Biomolecules, Montpellier I University, Montpellier, FranceAbstract: The purpose of this study was to investigate the influence of nano-sized β-tricalcium phosphate (β-TCP) on the biological performance of poly (lactic acid) (PLA) composite scaffolds by using in vitro degradation and an in vivo model of heterotopic bone formation. Nano-sized βTCP (nβ-TCP) was prepared with a wet grinding method from micro-sized β-TCP (mβ-TCP), and composite scaffolds containing 0, 10, 30, or 50 wt% nβ-TCP or 30 wt% mβ -TCP were generated using a freeze-drying method. Degradation was assessed by monitoring changes in microstructure, pH, weight, and compressive strength over a 26-week period of hydrolysis. Composite scaffolds were processed into blocks, and implanted into muscular pockets of rabbits after loading with recombinant human bone morphogenetic protein-2 (rhBMP-2). New bone formation was evaluated based on histological and immunohistochemical analysis 2, 4, and 8 weeks after implantation. The in vitro results indicated that the buffering effect of nβ-TCP was stronger than mβ-TCP, which was positively correlated with the content of nβ-TCP. The in vivo findings demonstrated that nβ-TCP enhanced the osteoconductivity of the scaffolds. Although composite scaffolds containing 30% nβ-TCP exhibited similar osteoconductivity to 50% nβ-TCP, they had better mechanical properties than the 50% nβ-TCP scaffolds. This study supports the potential application of a composite scaffold containing 30% nβ-TCP as a promising scaffold for bone regeneration.Keywords: poly (lactic acid), β-tricalcium phosphate, biodegradation, porous scaffold, bone regenerationhttp://www.dovepress.com/degradation-and-osteogenic-potential-of-a-novel-polylactic-acidnano-si-a11629 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Cao L Duan PG Wang HR Li XL Yuan FL Fan ZY Li SM Dong J |
spellingShingle |
Cao L Duan PG Wang HR Li XL Yuan FL Fan ZY Li SM Dong J Degradation and osteogenic potential of a novel poly(lactic acid)/nano-sized β-tricalcium phosphate scaffold International Journal of Nanomedicine |
author_facet |
Cao L Duan PG Wang HR Li XL Yuan FL Fan ZY Li SM Dong J |
author_sort |
Cao L |
title |
Degradation and osteogenic potential of a novel poly(lactic acid)/nano-sized β-tricalcium phosphate scaffold |
title_short |
Degradation and osteogenic potential of a novel poly(lactic acid)/nano-sized β-tricalcium phosphate scaffold |
title_full |
Degradation and osteogenic potential of a novel poly(lactic acid)/nano-sized β-tricalcium phosphate scaffold |
title_fullStr |
Degradation and osteogenic potential of a novel poly(lactic acid)/nano-sized β-tricalcium phosphate scaffold |
title_full_unstemmed |
Degradation and osteogenic potential of a novel poly(lactic acid)/nano-sized β-tricalcium phosphate scaffold |
title_sort |
degradation and osteogenic potential of a novel poly(lactic acid)/nano-sized β-tricalcium phosphate scaffold |
publisher |
Dove Medical Press |
series |
International Journal of Nanomedicine |
issn |
1176-9114 1178-2013 |
publishDate |
2012-11-01 |
description |
Lu Cao,1,2 Ping-Guo Duan,1,2 Hui-Ren Wang,1,2 Xi-Lei Li,1,2 Feng-Lai Yuan,3 Zhong-Yong Fan,4 Su-Ming Li,5 Jian Dong1,21Department of Orthopedic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China; 2State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai, China; 3Affiliated Third Hospital of Nantong University, Wuxi, Jiangsu, China; 4Department of Materials Science, Fudan University, Shanghai, China; 5Max Mousseron Institute on Biomolecules, Montpellier I University, Montpellier, FranceAbstract: The purpose of this study was to investigate the influence of nano-sized β-tricalcium phosphate (β-TCP) on the biological performance of poly (lactic acid) (PLA) composite scaffolds by using in vitro degradation and an in vivo model of heterotopic bone formation. Nano-sized βTCP (nβ-TCP) was prepared with a wet grinding method from micro-sized β-TCP (mβ-TCP), and composite scaffolds containing 0, 10, 30, or 50 wt% nβ-TCP or 30 wt% mβ -TCP were generated using a freeze-drying method. Degradation was assessed by monitoring changes in microstructure, pH, weight, and compressive strength over a 26-week period of hydrolysis. Composite scaffolds were processed into blocks, and implanted into muscular pockets of rabbits after loading with recombinant human bone morphogenetic protein-2 (rhBMP-2). New bone formation was evaluated based on histological and immunohistochemical analysis 2, 4, and 8 weeks after implantation. The in vitro results indicated that the buffering effect of nβ-TCP was stronger than mβ-TCP, which was positively correlated with the content of nβ-TCP. The in vivo findings demonstrated that nβ-TCP enhanced the osteoconductivity of the scaffolds. Although composite scaffolds containing 30% nβ-TCP exhibited similar osteoconductivity to 50% nβ-TCP, they had better mechanical properties than the 50% nβ-TCP scaffolds. This study supports the potential application of a composite scaffold containing 30% nβ-TCP as a promising scaffold for bone regeneration.Keywords: poly (lactic acid), β-tricalcium phosphate, biodegradation, porous scaffold, bone regeneration |
url |
http://www.dovepress.com/degradation-and-osteogenic-potential-of-a-novel-polylactic-acidnano-si-a11629 |
work_keys_str_mv |
AT caol degradationandosteogenicpotentialofanovelpolylacticacidnanosizedampbetatricalciumphosphatescaffold AT duanpg degradationandosteogenicpotentialofanovelpolylacticacidnanosizedampbetatricalciumphosphatescaffold AT wanghr degradationandosteogenicpotentialofanovelpolylacticacidnanosizedampbetatricalciumphosphatescaffold AT lixl degradationandosteogenicpotentialofanovelpolylacticacidnanosizedampbetatricalciumphosphatescaffold AT yuanfl degradationandosteogenicpotentialofanovelpolylacticacidnanosizedampbetatricalciumphosphatescaffold AT fanzy degradationandosteogenicpotentialofanovelpolylacticacidnanosizedampbetatricalciumphosphatescaffold AT lism degradationandosteogenicpotentialofanovelpolylacticacidnanosizedampbetatricalciumphosphatescaffold AT dongj degradationandosteogenicpotentialofanovelpolylacticacidnanosizedampbetatricalciumphosphatescaffold |
_version_ |
1716646701981237248 |