Development of Microfluidic Systems Enabling High-Throughput Single-Cell Protein Characterization

Development of Microfluidic Systems Enabling High-Throughput Single-Cell Protein Characterization

This article reviews recent developments in microfluidic systems enabling high-throughput characterization of single-cell proteins. Four key perspectives of microfluidic platforms are included in this review: (1) microfluidic fluorescent flow cytometry; (2) droplet based microfluidic flow cytometry;...

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Bibliographic Details
Main Authors: Beiyuan Fan, Xiufeng Li, Deyong Chen, Hongshang Peng, Junbo Wang, Jian Chen
Format: Article
Language:English
Published: MDPI AG 2016-02-01
Series:Sensors
Subjects:
microfluidics
single-cell analysis
protein characterization
high-throughput
Online Access:http://www.mdpi.com/1424-8220/16/2/232
Description
Summary:This article reviews recent developments in microfluidic systems enabling high-throughput characterization of single-cell proteins. Four key perspectives of microfluidic platforms are included in this review: (1) microfluidic fluorescent flow cytometry; (2) droplet based microfluidic flow cytometry; (3) large-array micro wells (microengraving); and (4) large-array micro chambers (barcode microchips). We examine the advantages and limitations of each technique and discuss future research opportunities by focusing on three key performance parameters (absolute quantification, sensitivity, and throughput).
ISSN:1424-8220

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