Development of Microfluidic Systems Enabling High-Throughput Single-Cell Protein Characterization

Development of Microfluidic Systems Enabling High-Throughput Single-Cell Protein Characterization

This article reviews recent developments in microfluidic systems enabling high-throughput characterization of single-cell proteins. Four key perspectives of microfluidic platforms are included in this review: (1) microfluidic fluorescent flow cytometry; (2) droplet based microfluidic flow cytometry;...

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Main Authors: Beiyuan Fan, Xiufeng Li, Deyong Chen, Hongshang Peng, Junbo Wang, Jian Chen
Format: Article
Language:English
Published: MDPI AG 2016-02-01
Series:Sensors
Subjects:
microfluidics
single-cell analysis
protein characterization
high-throughput
Online Access:http://www.mdpi.com/1424-8220/16/2/232
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spelling doaj-36ce5e424a30437ab349ed4ec91934a02020-11-24T23:20:20ZengMDPI AGSensors1424-82202016-02-0116223210.3390/s16020232s16020232Development of Microfluidic Systems Enabling High-Throughput Single-Cell Protein CharacterizationBeiyuan Fan0Xiufeng Li1Deyong Chen2Hongshang Peng3Junbo Wang4Jian Chen5State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, ChinaState Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, ChinaState Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, ChinaSchool of Science, Minzu University of China, Beijing 100081, ChinaState Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, ChinaState Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, ChinaThis article reviews recent developments in microfluidic systems enabling high-throughput characterization of single-cell proteins. Four key perspectives of microfluidic platforms are included in this review: (1) microfluidic fluorescent flow cytometry; (2) droplet based microfluidic flow cytometry; (3) large-array micro wells (microengraving); and (4) large-array micro chambers (barcode microchips). We examine the advantages and limitations of each technique and discuss future research opportunities by focusing on three key performance parameters (absolute quantification, sensitivity, and throughput).http://www.mdpi.com/1424-8220/16/2/232microfluidicssingle-cell analysisprotein characterizationhigh-throughput
collection DOAJ
language English
format Article
sources DOAJ
author Beiyuan Fan
Xiufeng Li
Deyong Chen
Hongshang Peng
Junbo Wang
Jian Chen
spellingShingle Beiyuan Fan
Xiufeng Li
Deyong Chen
Hongshang Peng
Junbo Wang
Jian Chen
Development of Microfluidic Systems Enabling High-Throughput Single-Cell Protein Characterization
Sensors
microfluidics
single-cell analysis
protein characterization
high-throughput
author_facet Beiyuan Fan
Xiufeng Li
Deyong Chen
Hongshang Peng
Junbo Wang
Jian Chen
author_sort Beiyuan Fan
title Development of Microfluidic Systems Enabling High-Throughput Single-Cell Protein Characterization
title_short Development of Microfluidic Systems Enabling High-Throughput Single-Cell Protein Characterization
title_full Development of Microfluidic Systems Enabling High-Throughput Single-Cell Protein Characterization
title_fullStr Development of Microfluidic Systems Enabling High-Throughput Single-Cell Protein Characterization
title_full_unstemmed Development of Microfluidic Systems Enabling High-Throughput Single-Cell Protein Characterization
title_sort development of microfluidic systems enabling high-throughput single-cell protein characterization
publisher MDPI AG
series Sensors
issn 1424-8220
publishDate 2016-02-01
description This article reviews recent developments in microfluidic systems enabling high-throughput characterization of single-cell proteins. Four key perspectives of microfluidic platforms are included in this review: (1) microfluidic fluorescent flow cytometry; (2) droplet based microfluidic flow cytometry; (3) large-array micro wells (microengraving); and (4) large-array micro chambers (barcode microchips). We examine the advantages and limitations of each technique and discuss future research opportunities by focusing on three key performance parameters (absolute quantification, sensitivity, and throughput).
topic microfluidics
single-cell analysis
protein characterization
high-throughput
url http://www.mdpi.com/1424-8220/16/2/232
work_keys_str_mv AT beiyuanfan developmentofmicrofluidicsystemsenablinghighthroughputsinglecellproteincharacterization
AT xiufengli developmentofmicrofluidicsystemsenablinghighthroughputsinglecellproteincharacterization
AT deyongchen developmentofmicrofluidicsystemsenablinghighthroughputsinglecellproteincharacterization
AT hongshangpeng developmentofmicrofluidicsystemsenablinghighthroughputsinglecellproteincharacterization
AT junbowang developmentofmicrofluidicsystemsenablinghighthroughputsinglecellproteincharacterization
AT jianchen developmentofmicrofluidicsystemsenablinghighthroughputsinglecellproteincharacterization
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