O-GlcNAcylation Enhances NUSAP1 Stability and Promotes Bladder Cancer Aggressiveness

• Main Authors: Chen Y, Liu J, Zhang W, Kadier A, Wang R, Zhang H, Yao X
• Format: Article
• Language: English
• Published: Dove Medical Press 2021-01-01
• Series: OncoTargets and Therapy
• Subjects: nusap1; o-glcnacylation; stability; bladder cancer; aggressiveness
• Online Access: https://www.dovepress.com/o-glcnacylation-enhances-nusap1-stability-and-promotes-bladder-cancer--peer-reviewed-article-OTT

Yifan Chen,* Ji Liu,* Wentao Zhang, Aimaitiaji Kadier,* Ruiliang Wang, Haimin Zhang, Xudong Yao Department of Urology, Shanghai Tenth People’s Hospital, Tongji University of Medicine, Shanghai, 200072, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xudong Yao; Haimin ZhangDepartment of Urology, Shanghai Tenth People’s Hospital, Tongji University of Medicine, Shanghai 200072, People’s Republic of ChinaEmail [e-mail]; [e-mail]: NUSAPl and O-GlcNAcylation were reported to be hyper-activated in many kinds of cancers and involved in the advanced progression of cancers. In bladder cancer, O-GlcNAc transferase (OGT) expresses in patients’ urine samples, with no expression in healthy individuals, indicating O-GlcNAcylation might involve in the occurrence and development of bladder cancer. Therefore, the present study aims to investigate the effects of O-GlcNAcylation in bladder cancer and if it can regulate NUSAP1 protein.Materials and Methods: Western blot, immunohistochemistry, and PCR were used to evaluate the protein expression and mRNA level of NUSAP1; CCK-8 and flow cytometry used to evaluate the proliferation and inhibited the apoptosis of bladder cancer.Results: The results showed that NUSAP1 was highly expressed in bladder cancer cells and tissue samples. NUSAP1 up-regulation significantly promoted the proliferation and inhibited the apoptosis of bladder cancer HT-1376 and T24 cells. Besides, the expression of O-GlcNAc was elevated in bladder cancer tissues and cells, and up-regulation of O-GlcNAc with GlcNAc and PuGNAc obviously increased NUSAP1 protein expression and stability. Moreover, knockdown OGT significantly inhibited the proliferation and tumorigenesis and promoted the apoptosis of bladder cancer cells, confirmed by CCK-8, in vivo xenotransplantation, and flow cytometry, whereas these roles were impaired when NUSAP1 was up-regulated.Conclusion: Overall, our study makes clear that hyper-O-GlcNAcylation accelerates bladder cancer progression through promotion of NUSAP1 expression and its stability.Keywords: NUSAP1, O-GlcNAcylation, stability, bladder cancer, aggressiveness