Convergent Evolution of Sodium Ion Selectivity in Metazoan Neuronal Signaling
Ion selectivity of metazoan voltage-gated Na+ channels is critical for neuronal signaling and has long been attributed to a ring of four conserved amino acids that constitute the ion selectivity filter (SF) at the channel pore. Yet, in addition to channels with a preference for Ca2+ ions, the expre...
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doaj-36fda1db149c4173a94ef52e824413292020-11-25T01:28:27ZengElsevierCell Reports2211-12472012-08-012224224810.1016/j.celrep.2012.06.016Convergent Evolution of Sodium Ion Selectivity in Metazoan Neuronal SignalingMaya Gur Barzilai0Adam M. Reitzel1Johanna E.M. Kraus2Dalia Gordon3Ulrich Technau4Michael Gurevitz5Yehu Moran6Department of Molecular Biology and Ecology of Plants, George S. Wise Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, IsraelBiology Department, Woods Hole Oceanographic Institution, Woods Hole, MA 02543, USADepartment of Molecular Evolution and Development, Faculty of Life Sciences, University of Vienna, Althanstraße 14, 1090 Vienna, AustriaDepartment of Molecular Biology and Ecology of Plants, George S. Wise Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, IsraelDepartment of Molecular Evolution and Development, Faculty of Life Sciences, University of Vienna, Althanstraße 14, 1090 Vienna, AustriaDepartment of Molecular Biology and Ecology of Plants, George S. Wise Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, IsraelDepartment of Molecular Evolution and Development, Faculty of Life Sciences, University of Vienna, Althanstraße 14, 1090 Vienna, Austria Ion selectivity of metazoan voltage-gated Na+ channels is critical for neuronal signaling and has long been attributed to a ring of four conserved amino acids that constitute the ion selectivity filter (SF) at the channel pore. Yet, in addition to channels with a preference for Ca2+ ions, the expression and characterization of Na+ channel homologs from the sea anemone Nematostella vectensis, a member of the early-branching metazoan phylum Cnidaria, revealed a sodium-selective channel bearing a noncanonical SF. Mutagenesis and physiological assays suggest that pore elements additional to the SF determine the preference for Na+ in this channel. Phylogenetic analysis assigns the Nematostella Na+-selective channel to a channel group unique to Cnidaria, which diverged >540 million years ago from Ca2+-conducting Na+ channel homologs. The identification of Cnidarian Na+-selective ion channels distinct from the channels of bilaterian animals indicates that selectivity for Na+ in neuronal signaling emerged independently in these two animal lineages. http://www.sciencedirect.com/science/article/pii/S2211124712001908 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Maya Gur Barzilai Adam M. Reitzel Johanna E.M. Kraus Dalia Gordon Ulrich Technau Michael Gurevitz Yehu Moran |
spellingShingle |
Maya Gur Barzilai Adam M. Reitzel Johanna E.M. Kraus Dalia Gordon Ulrich Technau Michael Gurevitz Yehu Moran Convergent Evolution of Sodium Ion Selectivity in Metazoan Neuronal Signaling Cell Reports |
author_facet |
Maya Gur Barzilai Adam M. Reitzel Johanna E.M. Kraus Dalia Gordon Ulrich Technau Michael Gurevitz Yehu Moran |
author_sort |
Maya Gur Barzilai |
title |
Convergent Evolution of Sodium Ion Selectivity in Metazoan Neuronal Signaling |
title_short |
Convergent Evolution of Sodium Ion Selectivity in Metazoan Neuronal Signaling |
title_full |
Convergent Evolution of Sodium Ion Selectivity in Metazoan Neuronal Signaling |
title_fullStr |
Convergent Evolution of Sodium Ion Selectivity in Metazoan Neuronal Signaling |
title_full_unstemmed |
Convergent Evolution of Sodium Ion Selectivity in Metazoan Neuronal Signaling |
title_sort |
convergent evolution of sodium ion selectivity in metazoan neuronal signaling |
publisher |
Elsevier |
series |
Cell Reports |
issn |
2211-1247 |
publishDate |
2012-08-01 |
description |
Ion selectivity of metazoan voltage-gated Na+ channels is critical for neuronal signaling and has long been attributed to a ring of four conserved amino acids that constitute the ion selectivity filter (SF) at the channel pore. Yet, in addition to channels with a preference for Ca2+ ions, the expression and characterization of Na+ channel homologs from the sea anemone Nematostella vectensis, a member of the early-branching metazoan phylum Cnidaria, revealed a sodium-selective channel bearing a noncanonical SF. Mutagenesis and physiological assays suggest that pore elements additional to the SF determine the preference for Na+ in this channel. Phylogenetic analysis assigns the Nematostella Na+-selective channel to a channel group unique to Cnidaria, which diverged >540 million years ago from Ca2+-conducting Na+ channel homologs. The identification of Cnidarian Na+-selective ion channels distinct from the channels of bilaterian animals indicates that selectivity for Na+ in neuronal signaling emerged independently in these two animal lineages.
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url |
http://www.sciencedirect.com/science/article/pii/S2211124712001908 |
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