Inhibition of Experimental Choroidal Neovascularization by a Novel Peptide Derived from Calreticulin Anti-Angiogenic Domain
Choroidal neovascularization (CNV) is a key pathological feature of several leading causes of vision loss including neovascular age-related macular degeneration. Here, we show that a calreticulin anti-angiogenic domain (CAD)-like peptide 27, CAD27, inhibited in vitro angiogenic activities, including...
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doaj-3725a24bb0174344b4ce92ed850bad9c2020-11-25T01:02:12ZengMDPI AGInternational Journal of Molecular Sciences1422-00672018-09-011910299310.3390/ijms19102993ijms19102993Inhibition of Experimental Choroidal Neovascularization by a Novel Peptide Derived from Calreticulin Anti-Angiogenic DomainYoun-Shen Bee0Yi-Ling Ma1Jinying Chen2Pei-Jhen Tsai3Shwu-Jiuan Sheu4Hsiu-Chen Lin5Hu Huang6Guei-Sheung Liu7Ming-Hong Tai8Department of Ophthalmology, Kaohsiung Veterans General Hospital, Kaohsiung 813, TaiwanDivision of Nephrology, Kaohsiung Veterans General Hospital, Kaohsiung 813, TaiwanMenzies Institute for Medical Research, University of Tasmania, Hobart, TAS 7000, AustraliaDepartment of Ophthalmology, Kaohsiung Veterans General Hospital, Kaohsiung 813, TaiwanDepartment of Ophthalmology, Kaohsiung Veterans General Hospital, Kaohsiung 813, TaiwanDepartment of Ophthalmology, Kaohsiung Veterans General Hospital, Kaohsiung 813, TaiwanAier Eye Institute, Aier School of Ophthalmology, Central South University, Changsha 410083, ChinaMenzies Institute for Medical Research, University of Tasmania, Hobart, TAS 7000, AustraliaDepartment of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung 804, TaiwanChoroidal neovascularization (CNV) is a key pathological feature of several leading causes of vision loss including neovascular age-related macular degeneration. Here, we show that a calreticulin anti-angiogenic domain (CAD)-like peptide 27, CAD27, inhibited in vitro angiogenic activities, including tube formation, migration of endothelial cells, and vascular sprouting from rat aortic ring explants. In a rat model of laser-induced CNV, we demonstrate that intravitreal injection of CAD27 significantly attenuated the formation of CNV lesions as measured via fundus fluorescein angiography and choroid flat-mounts (19.5% and 22.4% reductions at 10 μg and 20 μg of CAD27 injected, respectively). Similarly, the reduction of CNV lesions was observed in rats that had received topical applications of CAD27 (choroid flat-mounts: 17.9% and 32.5% reductions at 10 μg/mL and 20 μg/mL of CAD27 instilled, respectively). Retinal function was unaffected, as measured using electroretinography in both groups receiving interareal injection or topical applications of CAD27 for at least fourteen days. These findings show that CAD27 can be used as a potential therapeutic alternative for targeting CNV in diseases such as neovascular age-related macular degeneration.http://www.mdpi.com/1422-0067/19/10/2993choroidal neovascularizationneovascular age-related macular degenerationcalreticulin anti-angiogenic domain |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Youn-Shen Bee Yi-Ling Ma Jinying Chen Pei-Jhen Tsai Shwu-Jiuan Sheu Hsiu-Chen Lin Hu Huang Guei-Sheung Liu Ming-Hong Tai |
spellingShingle |
Youn-Shen Bee Yi-Ling Ma Jinying Chen Pei-Jhen Tsai Shwu-Jiuan Sheu Hsiu-Chen Lin Hu Huang Guei-Sheung Liu Ming-Hong Tai Inhibition of Experimental Choroidal Neovascularization by a Novel Peptide Derived from Calreticulin Anti-Angiogenic Domain International Journal of Molecular Sciences choroidal neovascularization neovascular age-related macular degeneration calreticulin anti-angiogenic domain |
author_facet |
Youn-Shen Bee Yi-Ling Ma Jinying Chen Pei-Jhen Tsai Shwu-Jiuan Sheu Hsiu-Chen Lin Hu Huang Guei-Sheung Liu Ming-Hong Tai |
author_sort |
Youn-Shen Bee |
title |
Inhibition of Experimental Choroidal Neovascularization by a Novel Peptide Derived from Calreticulin Anti-Angiogenic Domain |
title_short |
Inhibition of Experimental Choroidal Neovascularization by a Novel Peptide Derived from Calreticulin Anti-Angiogenic Domain |
title_full |
Inhibition of Experimental Choroidal Neovascularization by a Novel Peptide Derived from Calreticulin Anti-Angiogenic Domain |
title_fullStr |
Inhibition of Experimental Choroidal Neovascularization by a Novel Peptide Derived from Calreticulin Anti-Angiogenic Domain |
title_full_unstemmed |
Inhibition of Experimental Choroidal Neovascularization by a Novel Peptide Derived from Calreticulin Anti-Angiogenic Domain |
title_sort |
inhibition of experimental choroidal neovascularization by a novel peptide derived from calreticulin anti-angiogenic domain |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2018-09-01 |
description |
Choroidal neovascularization (CNV) is a key pathological feature of several leading causes of vision loss including neovascular age-related macular degeneration. Here, we show that a calreticulin anti-angiogenic domain (CAD)-like peptide 27, CAD27, inhibited in vitro angiogenic activities, including tube formation, migration of endothelial cells, and vascular sprouting from rat aortic ring explants. In a rat model of laser-induced CNV, we demonstrate that intravitreal injection of CAD27 significantly attenuated the formation of CNV lesions as measured via fundus fluorescein angiography and choroid flat-mounts (19.5% and 22.4% reductions at 10 μg and 20 μg of CAD27 injected, respectively). Similarly, the reduction of CNV lesions was observed in rats that had received topical applications of CAD27 (choroid flat-mounts: 17.9% and 32.5% reductions at 10 μg/mL and 20 μg/mL of CAD27 instilled, respectively). Retinal function was unaffected, as measured using electroretinography in both groups receiving interareal injection or topical applications of CAD27 for at least fourteen days. These findings show that CAD27 can be used as a potential therapeutic alternative for targeting CNV in diseases such as neovascular age-related macular degeneration. |
topic |
choroidal neovascularization neovascular age-related macular degeneration calreticulin anti-angiogenic domain |
url |
http://www.mdpi.com/1422-0067/19/10/2993 |
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