Immunity to endotoxin and Asp299Gly polymorphism of TLR-4 in adult patients with early and late onset of asthma
Aim. The gene polymorphism of Asp299Gly TLR-4 may be associated with the risk of asthma development. Methods and results. The gene polymorphism of TLR-4 (Asp299Gly) receptor has been researched in 262 early-onset and in 69 late-onset asthma patients. The state of anti-endotoxin immunity was asses...
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Zaporozhye State Medical University
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doaj-3730694e552443998359b43dfcc2d87a2020-11-24T22:33:52ZengZaporozhye State Medical UniversityZaporožskij Medicinskij Žurnal2306-41452310-12102015-06-0138690http://dx.doi.org/10.14739/2310-1210.2015.3.44506Immunity to endotoxin and Asp299Gly polymorphism of TLR-4 in adult patients with early and late onset of asthmaYu. A. Bisyuk A. I. KurchenkoV. E. KondratiukA. I. DubovyiAim. The gene polymorphism of Asp299Gly TLR-4 may be associated with the risk of asthma development. Methods and results. The gene polymorphism of TLR-4 (Asp299Gly) receptor has been researched in 262 early-onset and in 69 late-onset asthma patients. The state of anti-endotoxin immunity was assessed by determination of specific antibodies to the endotoxin of A, M, G classes and sCD14 by ELISA. The polymorphism was analyzed by the allele-specific polymerase chain reaction with electrophoretic detection. It was estimated that the risk of early-onset asthma in the population of Crimea is associated with genotypes AG and GG (Asp299Gly) of TLR-4. There were increased levels of anti-endotoxin IgM and decreased of sIgA in patients with late-onset asthma and AA genotype as compared to other genotypes. Conclusion. The gene polymorphism of Asp299Gly TLR-4 is associated with the risk of early-onset asthma development in Crimea population.http://zmj.zsmu.edu.ua/article/view/44506/42751Bronchial AsthmaEndotoxinsPolymorphismGenetic |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yu. A. Bisyuk A. I. Kurchenko V. E. Kondratiuk A. I. Dubovyi |
spellingShingle |
Yu. A. Bisyuk A. I. Kurchenko V. E. Kondratiuk A. I. Dubovyi Immunity to endotoxin and Asp299Gly polymorphism of TLR-4 in adult patients with early and late onset of asthma Zaporožskij Medicinskij Žurnal Bronchial Asthma Endotoxins Polymorphism Genetic |
author_facet |
Yu. A. Bisyuk A. I. Kurchenko V. E. Kondratiuk A. I. Dubovyi |
author_sort |
Yu. A. Bisyuk |
title |
Immunity to endotoxin and Asp299Gly polymorphism of TLR-4 in adult patients with early and late onset of asthma |
title_short |
Immunity to endotoxin and Asp299Gly polymorphism of TLR-4 in adult patients with early and late onset of asthma |
title_full |
Immunity to endotoxin and Asp299Gly polymorphism of TLR-4 in adult patients with early and late onset of asthma |
title_fullStr |
Immunity to endotoxin and Asp299Gly polymorphism of TLR-4 in adult patients with early and late onset of asthma |
title_full_unstemmed |
Immunity to endotoxin and Asp299Gly polymorphism of TLR-4 in adult patients with early and late onset of asthma |
title_sort |
immunity to endotoxin and asp299gly polymorphism of tlr-4 in adult patients with early and late onset of asthma |
publisher |
Zaporozhye State Medical University |
series |
Zaporožskij Medicinskij Žurnal |
issn |
2306-4145 2310-1210 |
publishDate |
2015-06-01 |
description |
Aim. The gene polymorphism of Asp299Gly TLR-4 may be associated with the risk of asthma development.
Methods and results. The gene polymorphism of TLR-4 (Asp299Gly) receptor has been researched in 262 early-onset and in 69 late-onset asthma patients. The state of anti-endotoxin immunity was assessed by determination of specific antibodies to the endotoxin of A, M, G classes and sCD14 by ELISA. The polymorphism was analyzed by the allele-specific polymerase chain reaction with electrophoretic detection. It was estimated that the risk of early-onset asthma in the population of Crimea is associated with genotypes AG and GG (Asp299Gly) of TLR-4. There were increased levels of anti-endotoxin IgM and decreased of sIgA in patients with late-onset asthma and AA genotype as compared to other genotypes.
Conclusion. The gene polymorphism of Asp299Gly TLR-4 is associated with the risk of early-onset asthma development in Crimea population. |
topic |
Bronchial Asthma Endotoxins Polymorphism Genetic |
url |
http://zmj.zsmu.edu.ua/article/view/44506/42751 |
work_keys_str_mv |
AT yuabisyuk immunitytoendotoxinandasp299glypolymorphismoftlr4inadultpatientswithearlyandlateonsetofasthma AT aikurchenko immunitytoendotoxinandasp299glypolymorphismoftlr4inadultpatientswithearlyandlateonsetofasthma AT vekondratiuk immunitytoendotoxinandasp299glypolymorphismoftlr4inadultpatientswithearlyandlateonsetofasthma AT aidubovyi immunitytoendotoxinandasp299glypolymorphismoftlr4inadultpatientswithearlyandlateonsetofasthma |
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1725728928745652224 |