Deciphering microbial diversity associated with Fusarium wilt-diseased and disease-free banana rhizosphere soil
Abstract Background Fusarium wilt of banana (Musa spp.) caused by the fungal pathogen Fusarium oxysporum f. sp. cubense (Foc) is a typical soilborne disease, that severely devastates the banana industry worldwide, and soil microbial diversity is closely related to the spread of Fusarium wilt. To und...
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doaj-37397853c36349cd9463dc75f9f34f0c2020-11-25T03:02:21ZengBMCBMC Microbiology1471-21802019-07-0119111310.1186/s12866-019-1531-6Deciphering microbial diversity associated with Fusarium wilt-diseased and disease-free banana rhizosphere soilDengbo Zhou0Tao Jing1Yufeng Chen2Fei Wang3Dengfeng Qi4Renjun Feng5Jianghui Xie6Huaping Li7Institute of Tropical Bioscience and Biotechnology, China Academy of Tropical Agricultural SciencesHaikou Experimental Station, China Academy of Tropical Agricultural SciencesInstitute of Tropical Bioscience and Biotechnology, China Academy of Tropical Agricultural SciencesInstitute of Tropical Bioscience and Biotechnology, China Academy of Tropical Agricultural SciencesInstitute of Tropical Bioscience and Biotechnology, China Academy of Tropical Agricultural SciencesInstitute of Tropical Bioscience and Biotechnology, China Academy of Tropical Agricultural SciencesInstitute of Tropical Bioscience and Biotechnology, China Academy of Tropical Agricultural SciencesGuangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Agriculture, South China Agricultural UniversityAbstract Background Fusarium wilt of banana (Musa spp.) caused by the fungal pathogen Fusarium oxysporum f. sp. cubense (Foc) is a typical soilborne disease, that severely devastates the banana industry worldwide, and soil microbial diversity is closely related to the spread of Fusarium wilt. To understand the relationship between microbial species and Fusarium wilt, it is important to understand the microbial diversity of the Fusarium wilt-diseased and disease-free soils from banana fields. Results Based on sequencing analysis of the bacterial 16S rRNA genes and fungal internal transcribed spacer (ITS) sequences, Foc abundance, fungal or bacterial richness and diversity were higher in the diseased soils than in the disease-free soils. Although Ascomycota and Zygomycota were the most abundant fungi phyla in all soil samples, Ascomycota abundance was significantly reduced in the disease-free soils. Mortierella (36.64%) was predominant in the disease-free soils. Regarding bacterial phyla, Proteobacteria, Acidobacteria, Chloroflexi, Firmicutes, Actinobacteria, Gemmatimonadetes, Bacteroidetes, Nitrospirae, Verrucomicrobia and Planctomycetes were dominant phyla in all soil samples. In particular, Firmicutes contributed 16.20% of the total abundance of disease-free soils. At the bacterial genus level, Bacillus, Lactococcus and Pseudomonas were abundant in disease-free soils with abundances of 8.20, 5.81 and 2.71%, respectively; lower abundances, of 4.12, 2.35 and 1.36%, respectively, were found in diseased soils. The distribution characteristics of fungal and bacterial genera may contribute to the abundance decrease of Foc in the disease-free soils. Conclusion Unique distributions of bacteria and fungi were observed in the diseased and disease-free soil samples from banana fields. These specific genera are useful for constructing a healthy microbial community structure of soil.http://link.springer.com/article/10.1186/s12866-019-1531-6Banana Fusarium wiltBacterial and fungal communitiesPathogen abundanceEnvironmental variables |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Dengbo Zhou Tao Jing Yufeng Chen Fei Wang Dengfeng Qi Renjun Feng Jianghui Xie Huaping Li |
spellingShingle |
Dengbo Zhou Tao Jing Yufeng Chen Fei Wang Dengfeng Qi Renjun Feng Jianghui Xie Huaping Li Deciphering microbial diversity associated with Fusarium wilt-diseased and disease-free banana rhizosphere soil BMC Microbiology Banana Fusarium wilt Bacterial and fungal communities Pathogen abundance Environmental variables |
author_facet |
Dengbo Zhou Tao Jing Yufeng Chen Fei Wang Dengfeng Qi Renjun Feng Jianghui Xie Huaping Li |
author_sort |
Dengbo Zhou |
title |
Deciphering microbial diversity associated with Fusarium wilt-diseased and disease-free banana rhizosphere soil |
title_short |
Deciphering microbial diversity associated with Fusarium wilt-diseased and disease-free banana rhizosphere soil |
title_full |
Deciphering microbial diversity associated with Fusarium wilt-diseased and disease-free banana rhizosphere soil |
title_fullStr |
Deciphering microbial diversity associated with Fusarium wilt-diseased and disease-free banana rhizosphere soil |
title_full_unstemmed |
Deciphering microbial diversity associated with Fusarium wilt-diseased and disease-free banana rhizosphere soil |
title_sort |
deciphering microbial diversity associated with fusarium wilt-diseased and disease-free banana rhizosphere soil |
publisher |
BMC |
series |
BMC Microbiology |
issn |
1471-2180 |
publishDate |
2019-07-01 |
description |
Abstract Background Fusarium wilt of banana (Musa spp.) caused by the fungal pathogen Fusarium oxysporum f. sp. cubense (Foc) is a typical soilborne disease, that severely devastates the banana industry worldwide, and soil microbial diversity is closely related to the spread of Fusarium wilt. To understand the relationship between microbial species and Fusarium wilt, it is important to understand the microbial diversity of the Fusarium wilt-diseased and disease-free soils from banana fields. Results Based on sequencing analysis of the bacterial 16S rRNA genes and fungal internal transcribed spacer (ITS) sequences, Foc abundance, fungal or bacterial richness and diversity were higher in the diseased soils than in the disease-free soils. Although Ascomycota and Zygomycota were the most abundant fungi phyla in all soil samples, Ascomycota abundance was significantly reduced in the disease-free soils. Mortierella (36.64%) was predominant in the disease-free soils. Regarding bacterial phyla, Proteobacteria, Acidobacteria, Chloroflexi, Firmicutes, Actinobacteria, Gemmatimonadetes, Bacteroidetes, Nitrospirae, Verrucomicrobia and Planctomycetes were dominant phyla in all soil samples. In particular, Firmicutes contributed 16.20% of the total abundance of disease-free soils. At the bacterial genus level, Bacillus, Lactococcus and Pseudomonas were abundant in disease-free soils with abundances of 8.20, 5.81 and 2.71%, respectively; lower abundances, of 4.12, 2.35 and 1.36%, respectively, were found in diseased soils. The distribution characteristics of fungal and bacterial genera may contribute to the abundance decrease of Foc in the disease-free soils. Conclusion Unique distributions of bacteria and fungi were observed in the diseased and disease-free soil samples from banana fields. These specific genera are useful for constructing a healthy microbial community structure of soil. |
topic |
Banana Fusarium wilt Bacterial and fungal communities Pathogen abundance Environmental variables |
url |
http://link.springer.com/article/10.1186/s12866-019-1531-6 |
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