Intraspecific Variation in <em>Armillaria</em> Species from Shrubs and Trees in Northwestern Spain
Until recently, the identification of Armillaria species relied upon morphological characteristics and mating tests, but now molecular techniques based on polymorphisms in the IGS region of the fungal rDNA are more commonly used, since these are more rapid and reliable. Differences found in RFLP pat...
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doaj-373d193280884424ae7d10ba4ca71cd92020-11-25T02:58:04ZengFirenze University PressPhytopathologia Mediterranea0031-94651593-20952004-08-0143210.14601/Phytopathol_Mediterr-17471743Intraspecific Variation in <em>Armillaria</em> Species from Shrubs and Trees in Northwestern SpainO. AguínJ.P. MansillaM.J. SainzUntil recently, the identification of Armillaria species relied upon morphological characteristics and mating tests, but now molecular techniques based on polymorphisms in the IGS region of the fungal rDNA are more commonly used, since these are more rapid and reliable. Differences found in RFLP patterns identifying Armillaria species have suggested the existence of intraspecific variation. In this work, 185 Armillaria isolates from different plant species (including fruit trees, broadleaf and coniferous trees, ornamental shrubs, kiwifruit and grapevine) affected by white root rot were analyzed by RFLP-PCR, in order to study intraspecific variation in Armillaria and the relationship with the plant host. Armillaria mellea was found in the majority of samples (71%), and was the most frequent Armillaria species in symptomatic ornamental shrubs, kiwifruit, grapevine, fruit trees and broadleaf trees. In conifers however white root rot was generally caused by Armillaria ostoyae. Armillaria gallica was identified, although with low incidence, in ornamental, coniferous, broadleaf and fruit hosts. Intraspecies variation was recorded only in A. mellea, for which RFLP patterns mel 1 and mel 2 were found. Most plants infected with A. mellea showed the mel 2 pattern. Further research is needed to study whether Armillaria RFLP patterns are specific to certain plant hosts, and whether intraspecific variation is related to differences in pathogenicity.https://oajournals.fupress.net/index.php/pm/article/view/5061 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
O. Aguín J.P. Mansilla M.J. Sainz |
spellingShingle |
O. Aguín J.P. Mansilla M.J. Sainz Intraspecific Variation in <em>Armillaria</em> Species from Shrubs and Trees in Northwestern Spain Phytopathologia Mediterranea |
author_facet |
O. Aguín J.P. Mansilla M.J. Sainz |
author_sort |
O. Aguín |
title |
Intraspecific Variation in <em>Armillaria</em> Species from Shrubs and Trees in Northwestern Spain |
title_short |
Intraspecific Variation in <em>Armillaria</em> Species from Shrubs and Trees in Northwestern Spain |
title_full |
Intraspecific Variation in <em>Armillaria</em> Species from Shrubs and Trees in Northwestern Spain |
title_fullStr |
Intraspecific Variation in <em>Armillaria</em> Species from Shrubs and Trees in Northwestern Spain |
title_full_unstemmed |
Intraspecific Variation in <em>Armillaria</em> Species from Shrubs and Trees in Northwestern Spain |
title_sort |
intraspecific variation in <em>armillaria</em> species from shrubs and trees in northwestern spain |
publisher |
Firenze University Press |
series |
Phytopathologia Mediterranea |
issn |
0031-9465 1593-2095 |
publishDate |
2004-08-01 |
description |
Until recently, the identification of Armillaria species relied upon morphological characteristics and
mating tests, but now molecular techniques based on polymorphisms in the IGS region of the fungal rDNA are more
commonly used, since these are more rapid and reliable. Differences found in RFLP patterns identifying Armillaria
species have suggested the existence of intraspecific variation. In this work, 185 Armillaria isolates from different
plant species (including fruit trees, broadleaf and coniferous trees, ornamental shrubs, kiwifruit and grapevine)
affected by white root rot were analyzed by RFLP-PCR, in order to study intraspecific variation in Armillaria and the
relationship with the plant host. Armillaria mellea was found in the majority of samples (71%), and was the most
frequent Armillaria species in symptomatic ornamental shrubs, kiwifruit, grapevine, fruit trees and broadleaf trees.
In conifers however white root rot was generally caused by Armillaria ostoyae. Armillaria gallica was identified,
although with low incidence, in ornamental, coniferous, broadleaf and fruit hosts. Intraspecies variation was recorded
only in A. mellea, for which RFLP patterns mel 1 and mel 2 were found. Most plants infected with A. mellea
showed the mel 2 pattern. Further research is needed to study whether Armillaria RFLP patterns are specific to
certain plant hosts, and whether intraspecific variation is related to differences in pathogenicity. |
url |
https://oajournals.fupress.net/index.php/pm/article/view/5061 |
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