Intraspecific Variation in <em>Armillaria</em> Species from Shrubs and Trees in Northwestern Spain

Until recently, the identification of Armillaria species relied upon morphological characteristics and mating tests, but now molecular techniques based on polymorphisms in the IGS region of the fungal rDNA are more commonly used, since these are more rapid and reliable. Differences found in RFLP pat...

Full description

Bibliographic Details
Main Authors: O. Aguín, J.P. Mansilla, M.J. Sainz
Format: Article
Language:English
Published: Firenze University Press 2004-08-01
Series:Phytopathologia Mediterranea
Online Access:https://oajournals.fupress.net/index.php/pm/article/view/5061
id doaj-373d193280884424ae7d10ba4ca71cd9
record_format Article
spelling doaj-373d193280884424ae7d10ba4ca71cd92020-11-25T02:58:04ZengFirenze University PressPhytopathologia Mediterranea0031-94651593-20952004-08-0143210.14601/Phytopathol_Mediterr-17471743Intraspecific Variation in <em>Armillaria</em> Species from Shrubs and Trees in Northwestern SpainO. AguínJ.P. MansillaM.J. SainzUntil recently, the identification of Armillaria species relied upon morphological characteristics and mating tests, but now molecular techniques based on polymorphisms in the IGS region of the fungal rDNA are more commonly used, since these are more rapid and reliable. Differences found in RFLP patterns identifying Armillaria species have suggested the existence of intraspecific variation. In this work, 185 Armillaria isolates from different plant species (including fruit trees, broadleaf and coniferous trees, ornamental shrubs, kiwifruit and grapevine) affected by white root rot were analyzed by RFLP-PCR, in order to study intraspecific variation in Armillaria and the relationship with the plant host. Armillaria mellea was found in the majority of samples (71%), and was the most frequent Armillaria species in symptomatic ornamental shrubs, kiwifruit, grapevine, fruit trees and broadleaf trees. In conifers however white root rot was generally caused by Armillaria ostoyae. Armillaria gallica was identified, although with low incidence, in ornamental, coniferous, broadleaf and fruit hosts. Intraspecies variation was recorded only in A. mellea, for which RFLP patterns mel 1 and mel 2 were found. Most plants infected with A. mellea showed the mel 2 pattern. Further research is needed to study whether Armillaria RFLP patterns are specific to certain plant hosts, and whether intraspecific variation is related to differences in pathogenicity.https://oajournals.fupress.net/index.php/pm/article/view/5061
collection DOAJ
language English
format Article
sources DOAJ
author O. Aguín
J.P. Mansilla
M.J. Sainz
spellingShingle O. Aguín
J.P. Mansilla
M.J. Sainz
Intraspecific Variation in <em>Armillaria</em> Species from Shrubs and Trees in Northwestern Spain
Phytopathologia Mediterranea
author_facet O. Aguín
J.P. Mansilla
M.J. Sainz
author_sort O. Aguín
title Intraspecific Variation in <em>Armillaria</em> Species from Shrubs and Trees in Northwestern Spain
title_short Intraspecific Variation in <em>Armillaria</em> Species from Shrubs and Trees in Northwestern Spain
title_full Intraspecific Variation in <em>Armillaria</em> Species from Shrubs and Trees in Northwestern Spain
title_fullStr Intraspecific Variation in <em>Armillaria</em> Species from Shrubs and Trees in Northwestern Spain
title_full_unstemmed Intraspecific Variation in <em>Armillaria</em> Species from Shrubs and Trees in Northwestern Spain
title_sort intraspecific variation in <em>armillaria</em> species from shrubs and trees in northwestern spain
publisher Firenze University Press
series Phytopathologia Mediterranea
issn 0031-9465
1593-2095
publishDate 2004-08-01
description Until recently, the identification of Armillaria species relied upon morphological characteristics and mating tests, but now molecular techniques based on polymorphisms in the IGS region of the fungal rDNA are more commonly used, since these are more rapid and reliable. Differences found in RFLP patterns identifying Armillaria species have suggested the existence of intraspecific variation. In this work, 185 Armillaria isolates from different plant species (including fruit trees, broadleaf and coniferous trees, ornamental shrubs, kiwifruit and grapevine) affected by white root rot were analyzed by RFLP-PCR, in order to study intraspecific variation in Armillaria and the relationship with the plant host. Armillaria mellea was found in the majority of samples (71%), and was the most frequent Armillaria species in symptomatic ornamental shrubs, kiwifruit, grapevine, fruit trees and broadleaf trees. In conifers however white root rot was generally caused by Armillaria ostoyae. Armillaria gallica was identified, although with low incidence, in ornamental, coniferous, broadleaf and fruit hosts. Intraspecies variation was recorded only in A. mellea, for which RFLP patterns mel 1 and mel 2 were found. Most plants infected with A. mellea showed the mel 2 pattern. Further research is needed to study whether Armillaria RFLP patterns are specific to certain plant hosts, and whether intraspecific variation is related to differences in pathogenicity.
url https://oajournals.fupress.net/index.php/pm/article/view/5061
work_keys_str_mv AT oaguin intraspecificvariationinemarmillariaemspeciesfromshrubsandtreesinnorthwesternspain
AT jpmansilla intraspecificvariationinemarmillariaemspeciesfromshrubsandtreesinnorthwesternspain
AT mjsainz intraspecificvariationinemarmillariaemspeciesfromshrubsandtreesinnorthwesternspain
_version_ 1724708704939409408