Antioxidant and anti-inflammatory activities of Orostachys japonicus
Objective: To determine the bioactive compounds of Orostachys japonicus (O. japonicus) (Maxim.) A. Berger extraction by different solvents (70% ethanol or water) and to evaluate the in vitro antioxidant and anti-inflammatory activities. Methods: Total polyphenol, flavonoid, and anthocyanin contents...
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Wolters Kluwer Medknow Publications
2020-01-01
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doaj-374ad40b5d454122a879377a3b05d86e2020-11-25T02:47:29ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912588-92222020-01-01101151652210.4103/2221-1691.294092Antioxidant and anti-inflammatory activities of Orostachys japonicusEun-Sun HwangNhuan Do ThiObjective: To determine the bioactive compounds of Orostachys japonicus (O. japonicus) (Maxim.) A. Berger extraction by different solvents (70% ethanol or water) and to evaluate the in vitro antioxidant and anti-inflammatory activities. Methods: Total polyphenol, flavonoid, and anthocyanin contents in O. japonicus extract were measured. Antioxidant activities were determined by DPPH, ABTS, and superoxide radical-scavenging ability assays. Anti-inflammatory activities were evaluated by nitric oxide production, tumor necrosis factor-α, and interleukin-6 expression techniques. Results: Extraction with 70% ethanol yielded the highest total polyphenol (60.03 mg/g dry weight) and flavonoid (55.50 mg/g dry weight) contents. The total anthocyanin contents in 70% ethanol and water extracts were 57.25 and 91.71 mg/g dry weight, respectively. The 70% ethanol extract also showed stronger antioxidant activity than the water extract. Antioxidant activity and reducing power increased with the increasing concentration of O. japonicus extract. O. japonicus extract at 0-400 μg/mL did not affect the growth of RAW 264.7 cells, whereas dose-dependent inhibition of nitric oxide, tumor necrosis factor-a, and interleukin-6 production was observed. Conclusions: O. japonicus inhibits oxidative and inflammatory reactions with potentially positive health-related effects.http://www.apjtb.org/article.asp?issn=2221-1691;year=2020;volume=10;issue=11;spage=516;epage=522;aulast=Hwangorostachys japonicus; antioxidant; raw 264.7; anti-inflammation; nitrix oxide |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Eun-Sun Hwang Nhuan Do Thi |
spellingShingle |
Eun-Sun Hwang Nhuan Do Thi Antioxidant and anti-inflammatory activities of Orostachys japonicus Asian Pacific Journal of Tropical Biomedicine orostachys japonicus; antioxidant; raw 264.7; anti-inflammation; nitrix oxide |
author_facet |
Eun-Sun Hwang Nhuan Do Thi |
author_sort |
Eun-Sun Hwang |
title |
Antioxidant and anti-inflammatory activities of Orostachys japonicus |
title_short |
Antioxidant and anti-inflammatory activities of Orostachys japonicus |
title_full |
Antioxidant and anti-inflammatory activities of Orostachys japonicus |
title_fullStr |
Antioxidant and anti-inflammatory activities of Orostachys japonicus |
title_full_unstemmed |
Antioxidant and anti-inflammatory activities of Orostachys japonicus |
title_sort |
antioxidant and anti-inflammatory activities of orostachys japonicus |
publisher |
Wolters Kluwer Medknow Publications |
series |
Asian Pacific Journal of Tropical Biomedicine |
issn |
2221-1691 2588-9222 |
publishDate |
2020-01-01 |
description |
Objective: To determine the bioactive compounds of Orostachys japonicus (O. japonicus) (Maxim.) A. Berger extraction by different solvents (70% ethanol or water) and to evaluate the in vitro antioxidant and anti-inflammatory activities.
Methods: Total polyphenol, flavonoid, and anthocyanin contents in O. japonicus extract were measured. Antioxidant activities were determined by DPPH, ABTS, and superoxide radical-scavenging ability assays. Anti-inflammatory activities were evaluated by nitric oxide production, tumor necrosis factor-α, and interleukin-6 expression techniques.
Results: Extraction with 70% ethanol yielded the highest total polyphenol (60.03 mg/g dry weight) and flavonoid (55.50 mg/g dry weight) contents. The total anthocyanin contents in 70% ethanol and water extracts were 57.25 and 91.71 mg/g dry weight, respectively. The 70% ethanol extract also showed stronger antioxidant activity than the water extract. Antioxidant activity and reducing power increased with the increasing concentration of O. japonicus extract. O. japonicus extract at 0-400 μg/mL did not affect the growth of RAW 264.7 cells, whereas dose-dependent inhibition of nitric oxide, tumor necrosis factor-a, and interleukin-6 production was observed.
Conclusions: O. japonicus inhibits oxidative and inflammatory reactions with potentially positive health-related effects. |
topic |
orostachys japonicus; antioxidant; raw 264.7; anti-inflammation; nitrix oxide |
url |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2020;volume=10;issue=11;spage=516;epage=522;aulast=Hwang |
work_keys_str_mv |
AT eunsunhwang antioxidantandantiinflammatoryactivitiesoforostachysjaponicus AT nhuandothi antioxidantandantiinflammatoryactivitiesoforostachysjaponicus |
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