Experimental study about the regulating effect of puerarin on osteoblast proliferation in vitro and related signal pathway expression
Objective: To study the regulating effect of puerarin on osteoblast proliferation in vitro and related signal pathway expression. Methods: Mouse osteoblasts MC353-E1 were cultured and divided into the puerarin group that were treated with 10 μg/mL puerarin and the control group that were treated...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Editorial Board of Journal of Hainan Medical University
2017-04-01
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| Series: | Journal of Hainan Medical University |
| Subjects: | |
| Online Access: | http://www.hnykdxxb.com/PDF/201707/4.pdf |
| Summary: | Objective: To study the regulating effect of puerarin on osteoblast proliferation in vitro and
related signal pathway expression. Methods: Mouse osteoblasts MC353-E1 were cultured
and divided into the puerarin group that were treated with 10 μg/mL puerarin and the control
group that were treated with medium without drugs or serum. Before treatment as well as 12,
24 and 48 h after treatment, MTS kits were used to determine the cell proliferation activity,
and enzyme-linked immunosorbent assay kits were used to determine the expression of PI3K/
AKT/FoxO signal pathway molecules and apoptosis genes. Results: 12, 24 and 48 h after
treatment, the cell viability of puerarin group were significantly higher than those of control
group, and p-PI3K, p-AKT, p-FoxO1 and p-FoxO3a expression were significantly higher than
those of control group while Bmi, Bax, CytC, AIF and Caspase-3 expression were significantly
lower than those of control group; puerarin had time-dependent regulating effect on the cell
viability as well as p-PI3K, p-AKT, p-FoxO1, p-FoxO3a, Bmi, Bax, CytC, AIF and Caspase-3
expression. Conclusion: Puerarin can promote osteoblast proliferation through PI3K/AKT/
FoxO signal pathways. |
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| ISSN: | 1007-1237 1007-1237 |