Effects of Hexane Root Extract of Ferula hermonis Boiss. on Human Breast and Colon Cancer Cells: An In Vitro and In Vivo Study
Breast and colon cancers are leading causes of cancer-related deaths globally. Plants are a potential source of natural products that may be used for the treatment of cancer. Ferula hermonis (FH) is reported to have diverse therapeutic effects. However, there are few reports on the in vitro anticanc...
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doaj-376b3508a49d444aa9d55ce7e98ac6262020-11-25T01:26:23ZengHindawi LimitedBioMed Research International2314-61332314-61412019-01-01201910.1155/2019/30798953079895Effects of Hexane Root Extract of Ferula hermonis Boiss. on Human Breast and Colon Cancer Cells: An In Vitro and In Vivo StudyNael Abutaha0Fahd A. Nasr1Mohammed Al-zharani2Ali S. Alqahtani3Omar M. Noman4Mohammed Mubarak5Semlali Abdelhabib6Muhammad A. Wadaan7Bioproducts Research Chair, Department of Zoology, College of Science, King Saud University, Riyadh, Saudi ArabiaMedicinal Aromatic and Poisonous Plants Research Center, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaAl Imam Mohammad Ibn Saud Islamic University (IMSIU), College of Science, Biology Department, Riyadh, Saudi ArabiaMedicinal Aromatic and Poisonous Plants Research Center, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaMedicinal Aromatic and Poisonous Plants Research Center, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaElectron Microscope Unit, King Saud University Medical City, Riyadh, Saudi ArabiaGroupe de Recherche en Écologie Buccale, Faculté de Médecine Dentaire, Université Laval, Québec, Québec, CanadaBioproducts Research Chair, Department of Zoology, College of Science, King Saud University, Riyadh, Saudi ArabiaBreast and colon cancers are leading causes of cancer-related deaths globally. Plants are a potential source of natural products that may be used for the treatment of cancer. Ferula hermonis (FH) is reported to have diverse therapeutic effects. However, there are few reports on the in vitro anticancer potential of FH extract. Our results showed that the Ferula hermonis root hexane extract (FHRH) can induce dose-dependent cytotoxic effects in breast and colon cancer cells with MTT IC50 values of 18.2 and 25 μg/ml, respectively. The FHRH extract induced apoptosis in both breast and colon cancer cells; this was confirmed by light and nuclear staining, q-PCR, and caspase 3/7 activation. This study also demonstrated the antitumor activity of FHRH in 9,10-dimethylbenz[α]anthracene DMBA-induced rodent mammary tumor model. The GC/MS analysis revealed the presence of 3,5-Dimethylbenzenemethanol, Alpha-Bisabolol, Alpha-pinene, Beta-pinene, and Baccatin III that have various pharmacological potentials. Overall, the present study suggests that FHRH extract possesses anticancer potential which is mediated through apoptotic effects in MDA-MB-231 and LoVo cells. The present study also considered a basis for further investigations into the potential use of FHRH extract as an anticancer therapy for breast and colon cancers.http://dx.doi.org/10.1155/2019/3079895 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nael Abutaha Fahd A. Nasr Mohammed Al-zharani Ali S. Alqahtani Omar M. Noman Mohammed Mubarak Semlali Abdelhabib Muhammad A. Wadaan |
spellingShingle |
Nael Abutaha Fahd A. Nasr Mohammed Al-zharani Ali S. Alqahtani Omar M. Noman Mohammed Mubarak Semlali Abdelhabib Muhammad A. Wadaan Effects of Hexane Root Extract of Ferula hermonis Boiss. on Human Breast and Colon Cancer Cells: An In Vitro and In Vivo Study BioMed Research International |
author_facet |
Nael Abutaha Fahd A. Nasr Mohammed Al-zharani Ali S. Alqahtani Omar M. Noman Mohammed Mubarak Semlali Abdelhabib Muhammad A. Wadaan |
author_sort |
Nael Abutaha |
title |
Effects of Hexane Root Extract of Ferula hermonis Boiss. on Human Breast and Colon Cancer Cells: An In Vitro and In Vivo Study |
title_short |
Effects of Hexane Root Extract of Ferula hermonis Boiss. on Human Breast and Colon Cancer Cells: An In Vitro and In Vivo Study |
title_full |
Effects of Hexane Root Extract of Ferula hermonis Boiss. on Human Breast and Colon Cancer Cells: An In Vitro and In Vivo Study |
title_fullStr |
Effects of Hexane Root Extract of Ferula hermonis Boiss. on Human Breast and Colon Cancer Cells: An In Vitro and In Vivo Study |
title_full_unstemmed |
Effects of Hexane Root Extract of Ferula hermonis Boiss. on Human Breast and Colon Cancer Cells: An In Vitro and In Vivo Study |
title_sort |
effects of hexane root extract of ferula hermonis boiss. on human breast and colon cancer cells: an in vitro and in vivo study |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2019-01-01 |
description |
Breast and colon cancers are leading causes of cancer-related deaths globally. Plants are a potential source of natural products that may be used for the treatment of cancer. Ferula hermonis (FH) is reported to have diverse therapeutic effects. However, there are few reports on the in vitro anticancer potential of FH extract. Our results showed that the Ferula hermonis root hexane extract (FHRH) can induce dose-dependent cytotoxic effects in breast and colon cancer cells with MTT IC50 values of 18.2 and 25 μg/ml, respectively. The FHRH extract induced apoptosis in both breast and colon cancer cells; this was confirmed by light and nuclear staining, q-PCR, and caspase 3/7 activation. This study also demonstrated the antitumor activity of FHRH in 9,10-dimethylbenz[α]anthracene DMBA-induced rodent mammary tumor model. The GC/MS analysis revealed the presence of 3,5-Dimethylbenzenemethanol, Alpha-Bisabolol, Alpha-pinene, Beta-pinene, and Baccatin III that have various pharmacological potentials. Overall, the present study suggests that FHRH extract possesses anticancer potential which is mediated through apoptotic effects in MDA-MB-231 and LoVo cells. The present study also considered a basis for further investigations into the potential use of FHRH extract as an anticancer therapy for breast and colon cancers. |
url |
http://dx.doi.org/10.1155/2019/3079895 |
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