Using time-resolved fluorescence to measure serum venom-specific IgE and IgG.

Using time-resolved fluorescence to measure serum venom-specific IgE and IgG.

We adapted DELFIA™ (dissociation-enhanced lanthanide fluoroimmunoassay), a time resolved fluorescence method, to quantitate whole venom specific and allergenic peptide-specific IgE (sIgE), sIgG(1) and sIgG(4) in serum from people clinically allergic to Australian native ant venoms, of which the pred...

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Main Authors: Pauline E van Eeden, Michael D Wiese, Susan Aulfrey, Belinda J Hales, Shelley F Stone, Simon G A Brown
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3031629?pdf=render
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spelling doaj-3780bf22d1ca448c96f882caf82f91d12020-11-25T02:00:17ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0161e1674110.1371/journal.pone.0016741Using time-resolved fluorescence to measure serum venom-specific IgE and IgG.Pauline E van EedenMichael D WieseSusan AulfreyBelinda J HalesShelley F StoneSimon G A BrownWe adapted DELFIA™ (dissociation-enhanced lanthanide fluoroimmunoassay), a time resolved fluorescence method, to quantitate whole venom specific and allergenic peptide-specific IgE (sIgE), sIgG(1) and sIgG(4) in serum from people clinically allergic to Australian native ant venoms, of which the predominant cause of allergy is jack jumper ant venom (JJAV). Intra-assay CV was 6.3% and inter-assay CV was 13.7% for JJAV sIgE. DELFIA and Phadia CAP JJAV sIgE results correlated well and had similar sensitivity and specificity for the detection of JJAV sIgE against intradermal skin testing as the gold standard. DELFIA was easily adapted for detecting sIgE to a panel of other native ant venoms.http://europepmc.org/articles/PMC3031629?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Pauline E van Eeden
Michael D Wiese
Susan Aulfrey
Belinda J Hales
Shelley F Stone
Simon G A Brown
spellingShingle Pauline E van Eeden
Michael D Wiese
Susan Aulfrey
Belinda J Hales
Shelley F Stone
Simon G A Brown
Using time-resolved fluorescence to measure serum venom-specific IgE and IgG.
PLoS ONE
author_facet Pauline E van Eeden
Michael D Wiese
Susan Aulfrey
Belinda J Hales
Shelley F Stone
Simon G A Brown
author_sort Pauline E van Eeden
title Using time-resolved fluorescence to measure serum venom-specific IgE and IgG.
title_short Using time-resolved fluorescence to measure serum venom-specific IgE and IgG.
title_full Using time-resolved fluorescence to measure serum venom-specific IgE and IgG.
title_fullStr Using time-resolved fluorescence to measure serum venom-specific IgE and IgG.
title_full_unstemmed Using time-resolved fluorescence to measure serum venom-specific IgE and IgG.
title_sort using time-resolved fluorescence to measure serum venom-specific ige and igg.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2011-01-01
description We adapted DELFIA™ (dissociation-enhanced lanthanide fluoroimmunoassay), a time resolved fluorescence method, to quantitate whole venom specific and allergenic peptide-specific IgE (sIgE), sIgG(1) and sIgG(4) in serum from people clinically allergic to Australian native ant venoms, of which the predominant cause of allergy is jack jumper ant venom (JJAV). Intra-assay CV was 6.3% and inter-assay CV was 13.7% for JJAV sIgE. DELFIA and Phadia CAP JJAV sIgE results correlated well and had similar sensitivity and specificity for the detection of JJAV sIgE against intradermal skin testing as the gold standard. DELFIA was easily adapted for detecting sIgE to a panel of other native ant venoms.
url http://europepmc.org/articles/PMC3031629?pdf=render
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AT susanaulfrey usingtimeresolvedfluorescencetomeasureserumvenomspecificigeandigg
AT belindajhales usingtimeresolvedfluorescencetomeasureserumvenomspecificigeandigg
AT shelleyfstone usingtimeresolvedfluorescencetomeasureserumvenomspecificigeandigg
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