Using time-resolved fluorescence to measure serum venom-specific IgE and IgG.
We adapted DELFIA™ (dissociation-enhanced lanthanide fluoroimmunoassay), a time resolved fluorescence method, to quantitate whole venom specific and allergenic peptide-specific IgE (sIgE), sIgG(1) and sIgG(4) in serum from people clinically allergic to Australian native ant venoms, of which the pred...
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2011-01-01
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doaj-3780bf22d1ca448c96f882caf82f91d12020-11-25T02:00:17ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0161e1674110.1371/journal.pone.0016741Using time-resolved fluorescence to measure serum venom-specific IgE and IgG.Pauline E van EedenMichael D WieseSusan AulfreyBelinda J HalesShelley F StoneSimon G A BrownWe adapted DELFIA™ (dissociation-enhanced lanthanide fluoroimmunoassay), a time resolved fluorescence method, to quantitate whole venom specific and allergenic peptide-specific IgE (sIgE), sIgG(1) and sIgG(4) in serum from people clinically allergic to Australian native ant venoms, of which the predominant cause of allergy is jack jumper ant venom (JJAV). Intra-assay CV was 6.3% and inter-assay CV was 13.7% for JJAV sIgE. DELFIA and Phadia CAP JJAV sIgE results correlated well and had similar sensitivity and specificity for the detection of JJAV sIgE against intradermal skin testing as the gold standard. DELFIA was easily adapted for detecting sIgE to a panel of other native ant venoms.http://europepmc.org/articles/PMC3031629?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pauline E van Eeden Michael D Wiese Susan Aulfrey Belinda J Hales Shelley F Stone Simon G A Brown |
spellingShingle |
Pauline E van Eeden Michael D Wiese Susan Aulfrey Belinda J Hales Shelley F Stone Simon G A Brown Using time-resolved fluorescence to measure serum venom-specific IgE and IgG. PLoS ONE |
author_facet |
Pauline E van Eeden Michael D Wiese Susan Aulfrey Belinda J Hales Shelley F Stone Simon G A Brown |
author_sort |
Pauline E van Eeden |
title |
Using time-resolved fluorescence to measure serum venom-specific IgE and IgG. |
title_short |
Using time-resolved fluorescence to measure serum venom-specific IgE and IgG. |
title_full |
Using time-resolved fluorescence to measure serum venom-specific IgE and IgG. |
title_fullStr |
Using time-resolved fluorescence to measure serum venom-specific IgE and IgG. |
title_full_unstemmed |
Using time-resolved fluorescence to measure serum venom-specific IgE and IgG. |
title_sort |
using time-resolved fluorescence to measure serum venom-specific ige and igg. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2011-01-01 |
description |
We adapted DELFIA™ (dissociation-enhanced lanthanide fluoroimmunoassay), a time resolved fluorescence method, to quantitate whole venom specific and allergenic peptide-specific IgE (sIgE), sIgG(1) and sIgG(4) in serum from people clinically allergic to Australian native ant venoms, of which the predominant cause of allergy is jack jumper ant venom (JJAV). Intra-assay CV was 6.3% and inter-assay CV was 13.7% for JJAV sIgE. DELFIA and Phadia CAP JJAV sIgE results correlated well and had similar sensitivity and specificity for the detection of JJAV sIgE against intradermal skin testing as the gold standard. DELFIA was easily adapted for detecting sIgE to a panel of other native ant venoms. |
url |
http://europepmc.org/articles/PMC3031629?pdf=render |
work_keys_str_mv |
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