RNA Interference by Cyanobacterial Feeding Demonstrates the <i>SCSG1</i> Gene Is Essential for Ciliogenesis during Oral Apparatus Regeneration in <i>Stentor</i>
In the giant ciliate <i>Stentor coeruleus</i>, oral apparatus (OA) regeneration is an experimentally tractable regeneration paradigm that occurs via a series of morphological steps. OA regeneration is thought to be driven by a complex regulatory system that orchestrates the temporal expr...
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doaj-3781424e319547e4b1ee4153e00854952021-01-16T00:03:03ZengMDPI AGMicroorganisms2076-26072021-01-01917617610.3390/microorganisms9010176RNA Interference by Cyanobacterial Feeding Demonstrates the <i>SCSG1</i> Gene Is Essential for Ciliogenesis during Oral Apparatus Regeneration in <i>Stentor</i>Wei Wei0Chuanqi Jiang1Xiaocui Chai2Juyuan Zhang3Cheng-Cai Zhang4Wei Miao5Jie Xiong6Key Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaKey Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaKey Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaKey Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaKey Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaKey Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaKey Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaIn the giant ciliate <i>Stentor coeruleus</i>, oral apparatus (OA) regeneration is an experimentally tractable regeneration paradigm that occurs via a series of morphological steps. OA regeneration is thought to be driven by a complex regulatory system that orchestrates the temporal expression of conserved and specific genes. We previously identified a <i>S. coeruleus</i>-specific gene (named <i>SCSG1</i>) that was significantly upregulated during the ciliogenesis stages of OA regeneration, with an expression peak at the stage of the first OA cilia appearance. We established a novel RNA interference (RNAi) method through cyanobacteria <i>Synechocystis</i> sp. PCC6803 feeding in <i>S. coeruleus</i>. The expression of <i>SCSG1</i> gene was significantly knocked down by using this method and induced abnormal ciliogenesis of OA regeneration in <i>S. coeruleus</i>, suggesting that <i>SCSG1</i> is essential for OA regeneration in <i>S. coeruleus</i>. This novel RNAi method by cyanobacterial feeding has potential utility for studying other ciliates.https://www.mdpi.com/2076-2607/9/1/176ciliatenovel RNAi method<i>Synechocystis</i> sp. PCC6803oral apparatus regeneration |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wei Wei Chuanqi Jiang Xiaocui Chai Juyuan Zhang Cheng-Cai Zhang Wei Miao Jie Xiong |
spellingShingle |
Wei Wei Chuanqi Jiang Xiaocui Chai Juyuan Zhang Cheng-Cai Zhang Wei Miao Jie Xiong RNA Interference by Cyanobacterial Feeding Demonstrates the <i>SCSG1</i> Gene Is Essential for Ciliogenesis during Oral Apparatus Regeneration in <i>Stentor</i> Microorganisms ciliate novel RNAi method <i>Synechocystis</i> sp. PCC6803 oral apparatus regeneration |
author_facet |
Wei Wei Chuanqi Jiang Xiaocui Chai Juyuan Zhang Cheng-Cai Zhang Wei Miao Jie Xiong |
author_sort |
Wei Wei |
title |
RNA Interference by Cyanobacterial Feeding Demonstrates the <i>SCSG1</i> Gene Is Essential for Ciliogenesis during Oral Apparatus Regeneration in <i>Stentor</i> |
title_short |
RNA Interference by Cyanobacterial Feeding Demonstrates the <i>SCSG1</i> Gene Is Essential for Ciliogenesis during Oral Apparatus Regeneration in <i>Stentor</i> |
title_full |
RNA Interference by Cyanobacterial Feeding Demonstrates the <i>SCSG1</i> Gene Is Essential for Ciliogenesis during Oral Apparatus Regeneration in <i>Stentor</i> |
title_fullStr |
RNA Interference by Cyanobacterial Feeding Demonstrates the <i>SCSG1</i> Gene Is Essential for Ciliogenesis during Oral Apparatus Regeneration in <i>Stentor</i> |
title_full_unstemmed |
RNA Interference by Cyanobacterial Feeding Demonstrates the <i>SCSG1</i> Gene Is Essential for Ciliogenesis during Oral Apparatus Regeneration in <i>Stentor</i> |
title_sort |
rna interference by cyanobacterial feeding demonstrates the <i>scsg1</i> gene is essential for ciliogenesis during oral apparatus regeneration in <i>stentor</i> |
publisher |
MDPI AG |
series |
Microorganisms |
issn |
2076-2607 |
publishDate |
2021-01-01 |
description |
In the giant ciliate <i>Stentor coeruleus</i>, oral apparatus (OA) regeneration is an experimentally tractable regeneration paradigm that occurs via a series of morphological steps. OA regeneration is thought to be driven by a complex regulatory system that orchestrates the temporal expression of conserved and specific genes. We previously identified a <i>S. coeruleus</i>-specific gene (named <i>SCSG1</i>) that was significantly upregulated during the ciliogenesis stages of OA regeneration, with an expression peak at the stage of the first OA cilia appearance. We established a novel RNA interference (RNAi) method through cyanobacteria <i>Synechocystis</i> sp. PCC6803 feeding in <i>S. coeruleus</i>. The expression of <i>SCSG1</i> gene was significantly knocked down by using this method and induced abnormal ciliogenesis of OA regeneration in <i>S. coeruleus</i>, suggesting that <i>SCSG1</i> is essential for OA regeneration in <i>S. coeruleus</i>. This novel RNAi method by cyanobacterial feeding has potential utility for studying other ciliates. |
topic |
ciliate novel RNAi method <i>Synechocystis</i> sp. PCC6803 oral apparatus regeneration |
url |
https://www.mdpi.com/2076-2607/9/1/176 |
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