Integrated Analysis of Dysregulated miRNA-Gene Expression in -Silenced Retinoblastoma Cells

• Main Authors: Nalini Venkatesan, P. R. Deepa, Madavan Vasudevan, Vikas Khetan, Ashwin M. Reddy, Subramanian Krishnakumar
• Format: Article
• Language: English
• Published: SAGE Publishing 2014-01-01
• Series: Bioinformatics and Biology Insights
• Online Access: https://doi.org/10.4137/BBI.S16958

Retinoblastoma (RB) is a primary childhood eye cancer. HMGA2 shows promise as a molecule for targeted therapy. The involvement of miRNAs in genome-level molecular dys-regulation in HMGA2 -silenced RB cells is poorly understood. Through miRNA expression microarray profiling, and an integrated array analysis of the HMGA2 -silenced RB cells, the dysregulated miRNAs and the miRNA-target relationships were modelled. Loop network analysis revealed a regulatory association between the transcription factor ( SOX5 ) and the deregulated miRNAs imiR-29a, miR-9*, miR-9-3 ). Silencing of HMGA2 deregulated the vital oncomirs ( miR-7, miR-331, miR-26a, miR-221, miR-17∼92 and miR-106b∼25 ) in RB cells. From this list, the role of the miR-106b∼25 cluster was examined further for its expression in primary RB tumor tissues (n = 20). The regulatory targets of miR-106b∼25 cluster namely p21 (cyclin-dependent kinase inhibitor) and BIM (pro-apoptotic gene) were elevated, and apoptotic cell death was observed, in RB tumor cells treated with the specific antagomirs of the miR-106b∼25 cluster. Thus, suppression of miR-106b∼25 cluster controls RB tumor growth. Taken together, HMGA2 mediated anti-tumor effect present in RB is, in part, mediated through the miR-106b∼25 cluster.