Molluscan Shell Matrix Characterization by Preparative SDS-PAGE
The glycoproteinaceous constituents of molluscan shell matrices usually resist chromatographical fractionation. We describe a protocol that overcomes this difficulty and permits collection of a large amount of shell proteins for further in vitro characterization. After dissolution of the mineral pha...
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Online Access: | http://dx.doi.org/10.1100/tsw.2003.30 |
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doaj-37a541f58bf5460cb9e9a001589b8b4a2020-11-25T00:28:17ZengHindawi LimitedThe Scientific World Journal1537-744X2003-01-01334234710.1100/tsw.2003.30Molluscan Shell Matrix Characterization by Preparative SDS-PAGEFrederic Marin0IsoTis, NV., ProfBronkhorstlaan, 10, Gebouw D, 3723 MB Bilthoven, The NetherlandsThe glycoproteinaceous constituents of molluscan shell matrices usually resist chromatographical fractionation. We describe a protocol that overcomes this difficulty and permits collection of a large amount of shell proteins for further in vitro characterization. After dissolution of the mineral phase, the glycoproteins are fractionated �blind� on a preparative electrophoresis. They are subsequently detected with a polyclonal antibody raised against the whole matrix.http://dx.doi.org/10.1100/tsw.2003.30 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Frederic Marin |
spellingShingle |
Frederic Marin Molluscan Shell Matrix Characterization by Preparative SDS-PAGE The Scientific World Journal |
author_facet |
Frederic Marin |
author_sort |
Frederic Marin |
title |
Molluscan Shell Matrix Characterization by Preparative SDS-PAGE |
title_short |
Molluscan Shell Matrix Characterization by Preparative SDS-PAGE |
title_full |
Molluscan Shell Matrix Characterization by Preparative SDS-PAGE |
title_fullStr |
Molluscan Shell Matrix Characterization by Preparative SDS-PAGE |
title_full_unstemmed |
Molluscan Shell Matrix Characterization by Preparative SDS-PAGE |
title_sort |
molluscan shell matrix characterization by preparative sds-page |
publisher |
Hindawi Limited |
series |
The Scientific World Journal |
issn |
1537-744X |
publishDate |
2003-01-01 |
description |
The glycoproteinaceous constituents of molluscan shell matrices usually resist chromatographical fractionation. We describe a protocol that overcomes this difficulty and permits collection of a large amount of shell proteins for further in vitro characterization. After dissolution of the mineral phase, the glycoproteins are fractionated �blind� on a preparative electrophoresis. They are subsequently detected with a polyclonal antibody raised against the whole matrix. |
url |
http://dx.doi.org/10.1100/tsw.2003.30 |
work_keys_str_mv |
AT fredericmarin molluscanshellmatrixcharacterizationbypreparativesdspage |
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1725336326070337536 |