Molluscan Shell Matrix Characterization by Preparative SDS-PAGE

Molluscan Shell Matrix Characterization by Preparative SDS-PAGE

The glycoproteinaceous constituents of molluscan shell matrices usually resist chromatographical fractionation. We describe a protocol that overcomes this difficulty and permits collection of a large amount of shell proteins for further in vitro characterization. After dissolution of the mineral pha...

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Main Author: Frederic Marin
Format: Article
Language:English
Published: Hindawi Limited 2003-01-01
Series:The Scientific World Journal
Online Access:http://dx.doi.org/10.1100/tsw.2003.30
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spelling doaj-37a541f58bf5460cb9e9a001589b8b4a2020-11-25T00:28:17ZengHindawi LimitedThe Scientific World Journal1537-744X2003-01-01334234710.1100/tsw.2003.30Molluscan Shell Matrix Characterization by Preparative SDS-PAGEFrederic Marin0IsoTis, NV., ProfBronkhorstlaan, 10, Gebouw D, 3723 MB Bilthoven, The NetherlandsThe glycoproteinaceous constituents of molluscan shell matrices usually resist chromatographical fractionation. We describe a protocol that overcomes this difficulty and permits collection of a large amount of shell proteins for further in vitro characterization. After dissolution of the mineral phase, the glycoproteins are fractionated �blind� on a preparative electrophoresis. They are subsequently detected with a polyclonal antibody raised against the whole matrix.http://dx.doi.org/10.1100/tsw.2003.30
collection DOAJ
language English
format Article
sources DOAJ
author Frederic Marin
spellingShingle Frederic Marin
Molluscan Shell Matrix Characterization by Preparative SDS-PAGE
The Scientific World Journal
author_facet Frederic Marin
author_sort Frederic Marin
title Molluscan Shell Matrix Characterization by Preparative SDS-PAGE
title_short Molluscan Shell Matrix Characterization by Preparative SDS-PAGE
title_full Molluscan Shell Matrix Characterization by Preparative SDS-PAGE
title_fullStr Molluscan Shell Matrix Characterization by Preparative SDS-PAGE
title_full_unstemmed Molluscan Shell Matrix Characterization by Preparative SDS-PAGE
title_sort molluscan shell matrix characterization by preparative sds-page
publisher Hindawi Limited
series The Scientific World Journal
issn 1537-744X
publishDate 2003-01-01
description The glycoproteinaceous constituents of molluscan shell matrices usually resist chromatographical fractionation. We describe a protocol that overcomes this difficulty and permits collection of a large amount of shell proteins for further in vitro characterization. After dissolution of the mineral phase, the glycoproteins are fractionated �blind� on a preparative electrophoresis. They are subsequently detected with a polyclonal antibody raised against the whole matrix.
url http://dx.doi.org/10.1100/tsw.2003.30
work_keys_str_mv AT fredericmarin molluscanshellmatrixcharacterizationbypreparativesdspage
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