Viability and molecular authentication of Coccidioides spp. isolates from the Instituto de Medicina Tropical de São Paulo culture collection, Brazil
Coccidioidomycosis is an emerging fungal disease in Brazil; adequate maintenance and authentication of Coccidioides isolates are essential for research into genetic diversity of the environmental organisms, as well as for understanding the human disease. Seventeen Coccidioides isolates maintained un...
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2013-02-01
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doaj-37b073a6f4ac456eb6132809380281f72020-11-24T22:41:51ZengUniversidade de São PauloRevista do Instituto de Medicina Tropical de São Paulo1678-99462013-02-0155171110.1590/S0036-46652013000100002S0036-46652013000100002Viability and molecular authentication of Coccidioides spp. isolates from the Instituto de Medicina Tropical de São Paulo culture collection, BrazilSarah Desirée Barbosa Cavalcanti0Mônica Scarpelli Martinelli Vidal1Maria da Glória Teixeira de Sousa2Gilda Maria Barbaro Del Negro3Universidade de São PauloUniversidade de São PauloUniversidade de São PauloUniversidade de São PauloCoccidioidomycosis is an emerging fungal disease in Brazil; adequate maintenance and authentication of Coccidioides isolates are essential for research into genetic diversity of the environmental organisms, as well as for understanding the human disease. Seventeen Coccidioides isolates maintained under mineral oil since 1975 in the Instituto de Medicina Tropical de São Paulo (IMTSP) culture collection, Brazil, were evaluated with respect to their viability, morphological characteristics and genetic features in order to authenticate these fungal cultures. Only five isolates were viable after almost 30 years, showing typical morphological characteristics, and sequencing analysis using Coi-F and Coi-R primers revealed 99% identity with Coccidioides genera. These five isolates were then preserved in liquid nitrogen and sterile water, and remained viable after two years of storage under these conditions, maintaining the same features.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652013000100002&lng=en&tlng=enCulture collectionCoccidioidesViabilityMolecular identificationLong-term preservation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sarah Desirée Barbosa Cavalcanti Mônica Scarpelli Martinelli Vidal Maria da Glória Teixeira de Sousa Gilda Maria Barbaro Del Negro |
spellingShingle |
Sarah Desirée Barbosa Cavalcanti Mônica Scarpelli Martinelli Vidal Maria da Glória Teixeira de Sousa Gilda Maria Barbaro Del Negro Viability and molecular authentication of Coccidioides spp. isolates from the Instituto de Medicina Tropical de São Paulo culture collection, Brazil Revista do Instituto de Medicina Tropical de São Paulo Culture collection Coccidioides Viability Molecular identification Long-term preservation |
author_facet |
Sarah Desirée Barbosa Cavalcanti Mônica Scarpelli Martinelli Vidal Maria da Glória Teixeira de Sousa Gilda Maria Barbaro Del Negro |
author_sort |
Sarah Desirée Barbosa Cavalcanti |
title |
Viability and molecular authentication of Coccidioides spp. isolates from the Instituto de Medicina Tropical de São Paulo culture collection, Brazil |
title_short |
Viability and molecular authentication of Coccidioides spp. isolates from the Instituto de Medicina Tropical de São Paulo culture collection, Brazil |
title_full |
Viability and molecular authentication of Coccidioides spp. isolates from the Instituto de Medicina Tropical de São Paulo culture collection, Brazil |
title_fullStr |
Viability and molecular authentication of Coccidioides spp. isolates from the Instituto de Medicina Tropical de São Paulo culture collection, Brazil |
title_full_unstemmed |
Viability and molecular authentication of Coccidioides spp. isolates from the Instituto de Medicina Tropical de São Paulo culture collection, Brazil |
title_sort |
viability and molecular authentication of coccidioides spp. isolates from the instituto de medicina tropical de são paulo culture collection, brazil |
publisher |
Universidade de São Paulo |
series |
Revista do Instituto de Medicina Tropical de São Paulo |
issn |
1678-9946 |
publishDate |
2013-02-01 |
description |
Coccidioidomycosis is an emerging fungal disease in Brazil; adequate maintenance and authentication of Coccidioides isolates are essential for research into genetic diversity of the environmental organisms, as well as for understanding the human disease. Seventeen Coccidioides isolates maintained under mineral oil since 1975 in the Instituto de Medicina Tropical de São Paulo (IMTSP) culture collection, Brazil, were evaluated with respect to their viability, morphological characteristics and genetic features in order to authenticate these fungal cultures. Only five isolates were viable after almost 30 years, showing typical morphological characteristics, and sequencing analysis using Coi-F and Coi-R primers revealed 99% identity with Coccidioides genera. These five isolates were then preserved in liquid nitrogen and sterile water, and remained viable after two years of storage under these conditions, maintaining the same features. |
topic |
Culture collection Coccidioides Viability Molecular identification Long-term preservation |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652013000100002&lng=en&tlng=en |
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