Preparation of radiolabeled GM2 and GA2 gangliosides.
GM2 and GA2 gangliosides from the brain of a patient who died of Sandhoff's disease were purified by solvent partition, silicic acid and silica gel column chromatography, and silica gel preparative thin-layer chromatography. They were tritiated in the terminal N-acetylgalactosamine residue usin...
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1979-07-01
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Series: | Journal of Lipid Research |
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doaj-37c1490448934a46b6dcebcc3919d4162021-04-24T05:52:48ZengElsevierJournal of Lipid Research0022-22751979-07-01205678681Preparation of radiolabeled GM2 and GA2 gangliosides.A NovakJ A LowdenY L GravelL S WolfeGM2 and GA2 gangliosides from the brain of a patient who died of Sandhoff's disease were purified by solvent partition, silicic acid and silica gel column chromatography, and silica gel preparative thin-layer chromatography. They were tritiated in the terminal N-acetylgalactosamine residue using galactose oxidase and sodium [3H]borohydride with the inclusion of catalase and peroxidase into the oxidation reaction. The specific activities were 4.62 X 10(8) dpm/mumol of GM2 ganglioside and 5.54 X 40(7) dpm/mumol of GA2 ganglioside. The addition of catalase and peroxidase to the tritiation procedure is recommended.http://www.sciencedirect.com/science/article/pii/S0022227520405930 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
A Novak J A Lowden Y L Gravel L S Wolfe |
spellingShingle |
A Novak J A Lowden Y L Gravel L S Wolfe Preparation of radiolabeled GM2 and GA2 gangliosides. Journal of Lipid Research |
author_facet |
A Novak J A Lowden Y L Gravel L S Wolfe |
author_sort |
A Novak |
title |
Preparation of radiolabeled GM2 and GA2 gangliosides. |
title_short |
Preparation of radiolabeled GM2 and GA2 gangliosides. |
title_full |
Preparation of radiolabeled GM2 and GA2 gangliosides. |
title_fullStr |
Preparation of radiolabeled GM2 and GA2 gangliosides. |
title_full_unstemmed |
Preparation of radiolabeled GM2 and GA2 gangliosides. |
title_sort |
preparation of radiolabeled gm2 and ga2 gangliosides. |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
1979-07-01 |
description |
GM2 and GA2 gangliosides from the brain of a patient who died of Sandhoff's disease were purified by solvent partition, silicic acid and silica gel column chromatography, and silica gel preparative thin-layer chromatography. They were tritiated in the terminal N-acetylgalactosamine residue using galactose oxidase and sodium [3H]borohydride with the inclusion of catalase and peroxidase into the oxidation reaction. The specific activities were 4.62 X 10(8) dpm/mumol of GM2 ganglioside and 5.54 X 40(7) dpm/mumol of GA2 ganglioside. The addition of catalase and peroxidase to the tritiation procedure is recommended. |
url |
http://www.sciencedirect.com/science/article/pii/S0022227520405930 |
work_keys_str_mv |
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