Preparation of radiolabeled GM2 and GA2 gangliosides.

Preparation of radiolabeled GM2 and GA2 gangliosides.

GM2 and GA2 gangliosides from the brain of a patient who died of Sandhoff's disease were purified by solvent partition, silicic acid and silica gel column chromatography, and silica gel preparative thin-layer chromatography. They were tritiated in the terminal N-acetylgalactosamine residue usin...

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Main Authors: A Novak, J A Lowden, Y L Gravel, L S Wolfe
Format: Article
Language:English
Published: Elsevier 1979-07-01
Series:Journal of Lipid Research
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520405930
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spelling doaj-37c1490448934a46b6dcebcc3919d4162021-04-24T05:52:48ZengElsevierJournal of Lipid Research0022-22751979-07-01205678681Preparation of radiolabeled GM2 and GA2 gangliosides.A NovakJ A LowdenY L GravelL S WolfeGM2 and GA2 gangliosides from the brain of a patient who died of Sandhoff's disease were purified by solvent partition, silicic acid and silica gel column chromatography, and silica gel preparative thin-layer chromatography. They were tritiated in the terminal N-acetylgalactosamine residue using galactose oxidase and sodium [3H]borohydride with the inclusion of catalase and peroxidase into the oxidation reaction. The specific activities were 4.62 X 10(8) dpm/mumol of GM2 ganglioside and 5.54 X 40(7) dpm/mumol of GA2 ganglioside. The addition of catalase and peroxidase to the tritiation procedure is recommended.http://www.sciencedirect.com/science/article/pii/S0022227520405930
collection DOAJ
language English
format Article
sources DOAJ
author A Novak
J A Lowden
Y L Gravel
L S Wolfe
spellingShingle A Novak
J A Lowden
Y L Gravel
L S Wolfe
Preparation of radiolabeled GM2 and GA2 gangliosides.
Journal of Lipid Research
author_facet A Novak
J A Lowden
Y L Gravel
L S Wolfe
author_sort A Novak
title Preparation of radiolabeled GM2 and GA2 gangliosides.
title_short Preparation of radiolabeled GM2 and GA2 gangliosides.
title_full Preparation of radiolabeled GM2 and GA2 gangliosides.
title_fullStr Preparation of radiolabeled GM2 and GA2 gangliosides.
title_full_unstemmed Preparation of radiolabeled GM2 and GA2 gangliosides.
title_sort preparation of radiolabeled gm2 and ga2 gangliosides.
publisher Elsevier
series Journal of Lipid Research
issn 0022-2275
publishDate 1979-07-01
description GM2 and GA2 gangliosides from the brain of a patient who died of Sandhoff's disease were purified by solvent partition, silicic acid and silica gel column chromatography, and silica gel preparative thin-layer chromatography. They were tritiated in the terminal N-acetylgalactosamine residue using galactose oxidase and sodium [3H]borohydride with the inclusion of catalase and peroxidase into the oxidation reaction. The specific activities were 4.62 X 10(8) dpm/mumol of GM2 ganglioside and 5.54 X 40(7) dpm/mumol of GA2 ganglioside. The addition of catalase and peroxidase to the tritiation procedure is recommended.
url http://www.sciencedirect.com/science/article/pii/S0022227520405930
work_keys_str_mv AT anovak preparationofradiolabeledgm2andga2gangliosides
AT jalowden preparationofradiolabeledgm2andga2gangliosides
AT ylgravel preparationofradiolabeledgm2andga2gangliosides
AT lswolfe preparationofradiolabeledgm2andga2gangliosides
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