Comparing the cytotoxicity of taurolidine, mitomycin C, and oxaliplatin on the proliferation of in vitro colon carcinoma cells following pressurized intra-peritoneal aerosol chemotherapy (PIPAC)
Abstract Background Besides its known antibacterial effect commonly used in intraperitoneal lavage, taurolidine has been observed to possess antineoplastic properties. In order to analyse this antineoplastic potential in a palliative therapeutic setting, taurolidine (TN) was compared to mitomycin C...
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doaj-37dd10168bf34ea2a14d714600edea902020-11-25T03:03:20ZengBMCWorld Journal of Surgical Oncology1477-78192019-06-011711710.1186/s12957-019-1633-5Comparing the cytotoxicity of taurolidine, mitomycin C, and oxaliplatin on the proliferation of in vitro colon carcinoma cells following pressurized intra-peritoneal aerosol chemotherapy (PIPAC)Justyna Schubert0Veria Khosrawipour1Haris Chaudhry2Mohamed Arafkas3Wolfram Trudo Knoefel4Alessio Pigazzi5Tanja Khosrawipour6Department of Food Hygiene and Consumer Health Protection, Wroclaw University of Environmental and Life SciencesDepartment of Orthopedic and Trauma Surgery, Ortho-Klinik DortmundDivision of Colorectal Surgery, Department of Surgery, University of California Irvine (UCI)Department of Plastic Surgery, Ortho-Klinik DortmundDepartment of Surgery (A), University-Hospital DüsseldorfDivision of Colorectal Surgery, Department of Surgery, University of California Irvine (UCI)Division of Colorectal Surgery, Department of Surgery, University of California Irvine (UCI)Abstract Background Besides its known antibacterial effect commonly used in intraperitoneal lavage, taurolidine has been observed to possess antineoplastic properties. In order to analyse this antineoplastic potential in a palliative therapeutic setting, taurolidine (TN) was compared to mitomycin C (MMC) and oxaliplatin (OX), known antineoplastic agents which are routinely used in intraperitoneal applications, following pressurized intra-peritoneal aerosol chemotherapy (PIPAC). Methods An in vitro model was established using a colon adenocarcinoma cell line (HT-29 human cells). Different experimental dosages of TN and combinations of TN, MMC, and OX were applied via PIPAC. To measure cell proliferation, a colorimetric tetrazolium reduction assay was utilized 24 h after PIPAC. Results We demonstrated a cytotoxic effect of TN and OX (184 mg/150 mL, p < 0.01) on tumor cell growth. An increasing dosage of TN (from 0.5 g/100 mL to 0.75 g/150 mL) correlated with higher cell toxicity when compared to untreated cells (p < 0.05 and p < 0.01, respectively). PIPAC with OX and both OX and TN (0.5 g/100 mL) showed the same cytotoxic effect (p < 0.01). No significant impact was observed for MMC (14 mg/50 mL, p > 0.05) or MMC with OX (p > 0.05) applied via PIPAC. Conclusions The intraperitoneal application of TN is mostly limited to lavage procedures in cases of peritonitis. Our results indicate a substantial antineoplastic in vitro effect on colon carcinoma cells following PIPAC application. While this effect could be used in the palliative treatment of peritoneal metastases, further clinical studies are required to investigate the feasibility of TN application in such cases.http://link.springer.com/article/10.1186/s12957-019-1633-5Colon carcinomaPressurized intra-peritoneal aerosol chemotherapy (PIPAC)Peritoneal metastasisTaurolidineMitomycin COxaliplatin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Justyna Schubert Veria Khosrawipour Haris Chaudhry Mohamed Arafkas Wolfram Trudo Knoefel Alessio Pigazzi Tanja Khosrawipour |
spellingShingle |
Justyna Schubert Veria Khosrawipour Haris Chaudhry Mohamed Arafkas Wolfram Trudo Knoefel Alessio Pigazzi Tanja Khosrawipour Comparing the cytotoxicity of taurolidine, mitomycin C, and oxaliplatin on the proliferation of in vitro colon carcinoma cells following pressurized intra-peritoneal aerosol chemotherapy (PIPAC) World Journal of Surgical Oncology Colon carcinoma Pressurized intra-peritoneal aerosol chemotherapy (PIPAC) Peritoneal metastasis Taurolidine Mitomycin C Oxaliplatin |
author_facet |
Justyna Schubert Veria Khosrawipour Haris Chaudhry Mohamed Arafkas Wolfram Trudo Knoefel Alessio Pigazzi Tanja Khosrawipour |
author_sort |
Justyna Schubert |
title |
Comparing the cytotoxicity of taurolidine, mitomycin C, and oxaliplatin on the proliferation of in vitro colon carcinoma cells following pressurized intra-peritoneal aerosol chemotherapy (PIPAC) |
title_short |
Comparing the cytotoxicity of taurolidine, mitomycin C, and oxaliplatin on the proliferation of in vitro colon carcinoma cells following pressurized intra-peritoneal aerosol chemotherapy (PIPAC) |
title_full |
Comparing the cytotoxicity of taurolidine, mitomycin C, and oxaliplatin on the proliferation of in vitro colon carcinoma cells following pressurized intra-peritoneal aerosol chemotherapy (PIPAC) |
title_fullStr |
Comparing the cytotoxicity of taurolidine, mitomycin C, and oxaliplatin on the proliferation of in vitro colon carcinoma cells following pressurized intra-peritoneal aerosol chemotherapy (PIPAC) |
title_full_unstemmed |
Comparing the cytotoxicity of taurolidine, mitomycin C, and oxaliplatin on the proliferation of in vitro colon carcinoma cells following pressurized intra-peritoneal aerosol chemotherapy (PIPAC) |
title_sort |
comparing the cytotoxicity of taurolidine, mitomycin c, and oxaliplatin on the proliferation of in vitro colon carcinoma cells following pressurized intra-peritoneal aerosol chemotherapy (pipac) |
publisher |
BMC |
series |
World Journal of Surgical Oncology |
issn |
1477-7819 |
publishDate |
2019-06-01 |
description |
Abstract Background Besides its known antibacterial effect commonly used in intraperitoneal lavage, taurolidine has been observed to possess antineoplastic properties. In order to analyse this antineoplastic potential in a palliative therapeutic setting, taurolidine (TN) was compared to mitomycin C (MMC) and oxaliplatin (OX), known antineoplastic agents which are routinely used in intraperitoneal applications, following pressurized intra-peritoneal aerosol chemotherapy (PIPAC). Methods An in vitro model was established using a colon adenocarcinoma cell line (HT-29 human cells). Different experimental dosages of TN and combinations of TN, MMC, and OX were applied via PIPAC. To measure cell proliferation, a colorimetric tetrazolium reduction assay was utilized 24 h after PIPAC. Results We demonstrated a cytotoxic effect of TN and OX (184 mg/150 mL, p < 0.01) on tumor cell growth. An increasing dosage of TN (from 0.5 g/100 mL to 0.75 g/150 mL) correlated with higher cell toxicity when compared to untreated cells (p < 0.05 and p < 0.01, respectively). PIPAC with OX and both OX and TN (0.5 g/100 mL) showed the same cytotoxic effect (p < 0.01). No significant impact was observed for MMC (14 mg/50 mL, p > 0.05) or MMC with OX (p > 0.05) applied via PIPAC. Conclusions The intraperitoneal application of TN is mostly limited to lavage procedures in cases of peritonitis. Our results indicate a substantial antineoplastic in vitro effect on colon carcinoma cells following PIPAC application. While this effect could be used in the palliative treatment of peritoneal metastases, further clinical studies are required to investigate the feasibility of TN application in such cases. |
topic |
Colon carcinoma Pressurized intra-peritoneal aerosol chemotherapy (PIPAC) Peritoneal metastasis Taurolidine Mitomycin C Oxaliplatin |
url |
http://link.springer.com/article/10.1186/s12957-019-1633-5 |
work_keys_str_mv |
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