Architectural Analysis of Picrosirius Red Stained Collagen in Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma using Polarization Microscopy
Introduction: Collagen degradation is important both for carcinogenesis and in its progression. Research regarding the co-relation of collagen with Oral Epithelial Dysplasia (OED) and Oral Squamous Cell Carcinoma (OSCC) is less explored. Aim: To elucidate the nature of collagen in Oral Epithelia...
Main Authors: | , , , , , , , |
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Format: | Article |
Language: | English |
Published: |
JCDR Research and Publications Private Limited
2015-12-01
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Series: | Journal of Clinical and Diagnostic Research |
Subjects: | |
Online Access: | https://jcdr.net/articles/PDF/6872/13476_CE[Ra1]_F(GH)_PF1(Ek_Om)_PFA(AK)_PF2(PAG).pdf |
Summary: | Introduction: Collagen degradation is important both for
carcinogenesis and in its progression. Research regarding the
co-relation of collagen with Oral Epithelial Dysplasia (OED) and
Oral Squamous Cell Carcinoma (OSCC) is less explored.
Aim: To elucidate the nature of collagen in Oral Epithelial
Dysplasia (OED) and Oral Squamous Cell Carcinoma (OSCC)
using Picrosirius Red Stain (PSR) under polarizing microscopy.
Materials and Methods: The study consisted of a total 40
samples which were divided into three groups. Group I included
buccal mucosa as negative and irritation fibroma as positive
control, group II consisted of OED and group III consisted
of Oral Squamous Cell Carcinoma (OSCC). A histochemical
analysis was conducted using PSR-polarization method by two
independent observers.
Results: The control group shows predominantly reddish–
orange birefringence. In OED with the advancement of grades,
the colour changed from yellowish-orange colour to yellowgreenish with progressive increase in greenish hue. As OSCC
regresses from well to poorly differentiated, the colour changed
from reddish-orange to yellowish orange to greenish-yellow
suggesting a transition from mature to immature collagen.
Conclusion: An observable gradual change in collagen of
both OED and OSCC was noted as they were proceeding from
benign to critical step. Thus, PSR is a useful tool for studying
stromal changes as supporting collagen shows the transition in
the form besides the alterations in epithelial cells. |
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ISSN: | 2249-782X 0973-709X |