Architectural Analysis of Picrosirius Red Stained Collagen in Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma using Polarization Microscopy

• Main Authors: Rashi Sharma, Shweta Rehani, Monica Mehendiratta, Priyanka Kardam, Madhumani Kumra, Yulia Mathias, Jyoti Yadav, Khushboo Sahay
• Format: Article
• Language: English
• Published: JCDR Research and Publications Private Limited 2015-12-01
• Series: Journal of Clinical and Diagnostic Research
• Subjects: birefringence; greenish yellow; reddish orange; yellowish orange
• Online Access: https://jcdr.net/articles/PDF/6872/13476_CE[Ra1]_F(GH)_PF1(Ek_Om)_PFA(AK)_PF2(PAG).pdf

Introduction: Collagen degradation is important both for carcinogenesis and in its progression. Research regarding the co-relation of collagen with Oral Epithelial Dysplasia (OED) and Oral Squamous Cell Carcinoma (OSCC) is less explored. Aim: To elucidate the nature of collagen in Oral Epithelial Dysplasia (OED) and Oral Squamous Cell Carcinoma (OSCC) using Picrosirius Red Stain (PSR) under polarizing microscopy. Materials and Methods: The study consisted of a total 40 samples which were divided into three groups. Group I included buccal mucosa as negative and irritation fibroma as positive control, group II consisted of OED and group III consisted of Oral Squamous Cell Carcinoma (OSCC). A histochemical analysis was conducted using PSR-polarization method by two independent observers. Results: The control group shows predominantly reddish– orange birefringence. In OED with the advancement of grades, the colour changed from yellowish-orange colour to yellowgreenish with progressive increase in greenish hue. As OSCC regresses from well to poorly differentiated, the colour changed from reddish-orange to yellowish orange to greenish-yellow suggesting a transition from mature to immature collagen. Conclusion: An observable gradual change in collagen of both OED and OSCC was noted as they were proceeding from benign to critical step. Thus, PSR is a useful tool for studying stromal changes as supporting collagen shows the transition in the form besides the alterations in epithelial cells.