| Summary: | Basketball is a sport in continuous evolution, being one of these key aspects of the players’ physical fitness that has an impact on the game. Therefore, this study aimed to characterize and identify the physical fitness level and profiles of basketball players according to sex. Total of 26 semi-professional basketball players were assessed (13 male, 13 female) through inertial devices in different previously validated fitness tests. T-test for independent samples and principal component analysis were used to analyze sex-related differences and to identify physical fitness profiles. The results showed differences according to sex in all physical fitness indexes (<i>p</i> < 0.01; <i>d</i> > 1.04) with higher values in males, except in accelerometer load during small-sided games (<i>p</i> = 0.17; <i>d</i> < 0.20). Four principal components were identified in male and female basketball players, being two common ([<i>PC1</i>] aerobic capacity and in-game physical conditioning, [<i>PC4</i> male, <i>PC3</i> female] unipodal jump performance) and two different profiles (male: [<i>PC2</i>] bipodal jump capacity and acceleration, [<i>PC3</i>] curvilinear displacement; female: [<i>PC2</i>] bipodal jump capacity and curvilinear displacement, [<i>PC4</i>] deceleration). In conclusion, training design must be different and individualized according to different variables, including physical fitness profiles between them. For practical applications, these results will allow knowing the advantages and weaknesses of each athlete to adapt training tasks and game systems based on the skills and capabilities of the players in basketball.
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