Rhizoplane Bacteria and Plant Species Co-determine Phosphorus-Mediated Microbial Legacy Effect
Much effort has been directed toward increasing the availability of soil residual phosphorus (P). However, little information is available for the P fertilization-induced biotic P legacy and its mediation of plant P uptake. We collected microbial inocula from a monoculture maize field site with a 10...
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doaj-381e8bc9039544a1928a2e38faec13822020-11-25T02:07:52ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-12-011010.3389/fmicb.2019.02856478972Rhizoplane Bacteria and Plant Species Co-determine Phosphorus-Mediated Microbial Legacy EffectMing Lang0Ming Lang1Ming Lang2Shuikuan Bei3Shuikuan Bei4Xia Li5Xia Li6Xia Li7Thomas W. Kuyper8Junling Zhang9Junling Zhang10College of Resources and Environment, Southwest University, Chongqing, ChinaCentre for Resources, Environment and Food Security, College of Resources and Environmental Sciences, China Agricultural University, Beijing, ChinaKey Laboratory of Plant-Soil Interactions, Ministry of Education, Beijing, ChinaCentre for Resources, Environment and Food Security, College of Resources and Environmental Sciences, China Agricultural University, Beijing, ChinaKey Laboratory of Plant-Soil Interactions, Ministry of Education, Beijing, ChinaCentre for Resources, Environment and Food Security, College of Resources and Environmental Sciences, China Agricultural University, Beijing, ChinaKey Laboratory of Plant-Soil Interactions, Ministry of Education, Beijing, ChinaSchool of Life Science, Shanxi Datong University, Datong, ChinaSoil Biology Group, Wageningen University, Wageningen, NetherlandsCentre for Resources, Environment and Food Security, College of Resources and Environmental Sciences, China Agricultural University, Beijing, ChinaKey Laboratory of Plant-Soil Interactions, Ministry of Education, Beijing, ChinaMuch effort has been directed toward increasing the availability of soil residual phosphorus (P). However, little information is available for the P fertilization-induced biotic P legacy and its mediation of plant P uptake. We collected microbial inocula from a monoculture maize field site with a 10-year P-fertilization history. A greenhouse experiment was conducted to investigate whether bacterial communities, as a result of different P-fertilization history (nil P, 33 and/or 131 kg P kg ha–1 yr–1), affected the growth of a conspecific (maize) or heterospecific (clover) plant, at two levels of current P application (5 and 30 mg P kg–1 soil; P5 and P30). Deep amplicon sequencing (16S rRNA) was used to determine the maize and clover root-associated bacterial microbiome in different rhizocompartments (rhizoplane, rhizosphere, bulk soil). For both maize and clover, rhizocompartment and host identity were the dominant factors shaping bacterial assemblages, followed by P supply level and the inoculum effect was smallest. Bacterial operational taxonomic unit (OTU) numbers decreased from bulk soil to rhizoplane, whilst specific OTUs were enriched from bulk soil to rhizoplane. A clear hierarchical habitat filtering of bacterial communities was observed in the rhizoplane of the two plant species. The functional prediction of dominant bacterial taxa in the rhizoplane differed between clover and maize, and clover microbiota were more closely associated with P metabolism and maize with carbon cycling. More connected and complex interactions were observed in the clover rhizoplane compared to maize. The microbial legacy effect caused by long-term P fertilization is overridden by host identity and rhizocompartment. Our results highlight the importance of crop diversification in improving P efficiency. The fine-tuning of rhizosphere microbiome in host metabolism indicates that the functions of microbial communities should be integrated into P management to increase P use efficiency and sustainable food production.https://www.frontiersin.org/article/10.3389/fmicb.2019.02856/fullsoil microbiomehigh-throughput sequencingmicrobial ecological networkrhizocompartmentlong-term phosphorus fertilization |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ming Lang Ming Lang Ming Lang Shuikuan Bei Shuikuan Bei Xia Li Xia Li Xia Li Thomas W. Kuyper Junling Zhang Junling Zhang |
spellingShingle |
Ming Lang Ming Lang Ming Lang Shuikuan Bei Shuikuan Bei Xia Li Xia Li Xia Li Thomas W. Kuyper Junling Zhang Junling Zhang Rhizoplane Bacteria and Plant Species Co-determine Phosphorus-Mediated Microbial Legacy Effect Frontiers in Microbiology soil microbiome high-throughput sequencing microbial ecological network rhizocompartment long-term phosphorus fertilization |
author_facet |
Ming Lang Ming Lang Ming Lang Shuikuan Bei Shuikuan Bei Xia Li Xia Li Xia Li Thomas W. Kuyper Junling Zhang Junling Zhang |
author_sort |
Ming Lang |
title |
Rhizoplane Bacteria and Plant Species Co-determine Phosphorus-Mediated Microbial Legacy Effect |
title_short |
Rhizoplane Bacteria and Plant Species Co-determine Phosphorus-Mediated Microbial Legacy Effect |
title_full |
Rhizoplane Bacteria and Plant Species Co-determine Phosphorus-Mediated Microbial Legacy Effect |
title_fullStr |
Rhizoplane Bacteria and Plant Species Co-determine Phosphorus-Mediated Microbial Legacy Effect |
title_full_unstemmed |
Rhizoplane Bacteria and Plant Species Co-determine Phosphorus-Mediated Microbial Legacy Effect |
title_sort |
rhizoplane bacteria and plant species co-determine phosphorus-mediated microbial legacy effect |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2019-12-01 |
description |
Much effort has been directed toward increasing the availability of soil residual phosphorus (P). However, little information is available for the P fertilization-induced biotic P legacy and its mediation of plant P uptake. We collected microbial inocula from a monoculture maize field site with a 10-year P-fertilization history. A greenhouse experiment was conducted to investigate whether bacterial communities, as a result of different P-fertilization history (nil P, 33 and/or 131 kg P kg ha–1 yr–1), affected the growth of a conspecific (maize) or heterospecific (clover) plant, at two levels of current P application (5 and 30 mg P kg–1 soil; P5 and P30). Deep amplicon sequencing (16S rRNA) was used to determine the maize and clover root-associated bacterial microbiome in different rhizocompartments (rhizoplane, rhizosphere, bulk soil). For both maize and clover, rhizocompartment and host identity were the dominant factors shaping bacterial assemblages, followed by P supply level and the inoculum effect was smallest. Bacterial operational taxonomic unit (OTU) numbers decreased from bulk soil to rhizoplane, whilst specific OTUs were enriched from bulk soil to rhizoplane. A clear hierarchical habitat filtering of bacterial communities was observed in the rhizoplane of the two plant species. The functional prediction of dominant bacterial taxa in the rhizoplane differed between clover and maize, and clover microbiota were more closely associated with P metabolism and maize with carbon cycling. More connected and complex interactions were observed in the clover rhizoplane compared to maize. The microbial legacy effect caused by long-term P fertilization is overridden by host identity and rhizocompartment. Our results highlight the importance of crop diversification in improving P efficiency. The fine-tuning of rhizosphere microbiome in host metabolism indicates that the functions of microbial communities should be integrated into P management to increase P use efficiency and sustainable food production. |
topic |
soil microbiome high-throughput sequencing microbial ecological network rhizocompartment long-term phosphorus fertilization |
url |
https://www.frontiersin.org/article/10.3389/fmicb.2019.02856/full |
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