TAILOR-MADE BIOCATALYSTS: COMBINING THERMODYNAMICS, ORGANIC SYNTHESIS, MOLECULAR BIOLOGY, BIOCHEMISTRY AND MICROBIOLOGY FOR THE DESIGN OF ENZYME SELECTIONS

TAILOR-MADE BIOCATALYSTS: COMBINING THERMODYNAMICS, ORGANIC SYNTHESIS, MOLECULAR BIOLOGY, BIOCHEMISTRY AND MICROBIOLOGY FOR THE DESIGN OF ENZYME SELECTIONS

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A general strategy for the isolation of catalysts for given chemical reactions was designed. A first link between genes and their corresponding proteins was established by phage display: using Darwin's principles on evolution based on selection and amplification, rare protein molecules can then...

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Main Author: Jean-Luc Jestin
Format: Article
Language:English
Published: Elsevier 2012-09-01
Series:Computational and Structural Biotechnology Journal
Online Access:http://www.sciencedirect.com/science/article/pii/S200103701460091X
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spelling doaj-38208c44da554f7c8e386596541feccb2020-11-24T21:23:48ZengElsevierComputational and Structural Biotechnology Journal2001-03702012-09-012310.5936/csbj.201209013TAILOR-MADE BIOCATALYSTS: COMBINING THERMODYNAMICS, ORGANIC SYNTHESIS, MOLECULAR BIOLOGY, BIOCHEMISTRY AND MICROBIOLOGY FOR THE DESIGN OF ENZYME SELECTIONSJean-Luc Jestin0Unité de Virologie Structurale, Département de Virologie, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, FranceA general strategy for the isolation of catalysts for given chemical reactions was designed. A first link between genes and their corresponding proteins was established by phage display: using Darwin's principles on evolution based on selection and amplification, rare protein molecules can then be selected for function from a large repertoire prior to their characterization by sequencing of their genes. A second link was created between enzymes and their products. By making use of the chelate effect and of Inovirus particles as a chemical, affinity chromatography for the reaction product is then sufficient to isolate among 106 to 1011 proteins and their genes, the rare ones coding for catalysts of interest. The strategy for the parallel processing of information on the catalytic activity of variants from a large protein repertoire is highlighted in this review.http://www.sciencedirect.com/science/article/pii/S200103701460091X
collection DOAJ
language English
format Article
sources DOAJ
author Jean-Luc Jestin
spellingShingle Jean-Luc Jestin
TAILOR-MADE BIOCATALYSTS: COMBINING THERMODYNAMICS, ORGANIC SYNTHESIS, MOLECULAR BIOLOGY, BIOCHEMISTRY AND MICROBIOLOGY FOR THE DESIGN OF ENZYME SELECTIONS
Computational and Structural Biotechnology Journal
author_facet Jean-Luc Jestin
author_sort Jean-Luc Jestin
title TAILOR-MADE BIOCATALYSTS: COMBINING THERMODYNAMICS, ORGANIC SYNTHESIS, MOLECULAR BIOLOGY, BIOCHEMISTRY AND MICROBIOLOGY FOR THE DESIGN OF ENZYME SELECTIONS
title_short TAILOR-MADE BIOCATALYSTS: COMBINING THERMODYNAMICS, ORGANIC SYNTHESIS, MOLECULAR BIOLOGY, BIOCHEMISTRY AND MICROBIOLOGY FOR THE DESIGN OF ENZYME SELECTIONS
title_full TAILOR-MADE BIOCATALYSTS: COMBINING THERMODYNAMICS, ORGANIC SYNTHESIS, MOLECULAR BIOLOGY, BIOCHEMISTRY AND MICROBIOLOGY FOR THE DESIGN OF ENZYME SELECTIONS
title_fullStr TAILOR-MADE BIOCATALYSTS: COMBINING THERMODYNAMICS, ORGANIC SYNTHESIS, MOLECULAR BIOLOGY, BIOCHEMISTRY AND MICROBIOLOGY FOR THE DESIGN OF ENZYME SELECTIONS
title_full_unstemmed TAILOR-MADE BIOCATALYSTS: COMBINING THERMODYNAMICS, ORGANIC SYNTHESIS, MOLECULAR BIOLOGY, BIOCHEMISTRY AND MICROBIOLOGY FOR THE DESIGN OF ENZYME SELECTIONS
title_sort tailor-made biocatalysts: combining thermodynamics, organic synthesis, molecular biology, biochemistry and microbiology for the design of enzyme selections
publisher Elsevier
series Computational and Structural Biotechnology Journal
issn 2001-0370
publishDate 2012-09-01
description A general strategy for the isolation of catalysts for given chemical reactions was designed. A first link between genes and their corresponding proteins was established by phage display: using Darwin's principles on evolution based on selection and amplification, rare protein molecules can then be selected for function from a large repertoire prior to their characterization by sequencing of their genes. A second link was created between enzymes and their products. By making use of the chelate effect and of Inovirus particles as a chemical, affinity chromatography for the reaction product is then sufficient to isolate among 106 to 1011 proteins and their genes, the rare ones coding for catalysts of interest. The strategy for the parallel processing of information on the catalytic activity of variants from a large protein repertoire is highlighted in this review.
url http://www.sciencedirect.com/science/article/pii/S200103701460091X
work_keys_str_mv AT jeanlucjestin tailormadebiocatalystscombiningthermodynamicsorganicsynthesismolecularbiologybiochemistryandmicrobiologyforthedesignofenzymeselections
_version_ 1725991095482974208

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