Inhibition kinetics of digestive proteases for Anticarsia gemmatalis
Abstract Anticarsia gemmatalis Hübner, 1818 (Lepidoptera) is a major pest of soybean in the Brazil. It is known that the reduction of proteolytic activity by the ingestion of protease inhibitors reduces digestion and larval development of the insects. Control via inhibition of the digestive enzymes...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Academia Brasileira de Ciências
|
Series: | Anais da Academia Brasileira de Ciências |
Subjects: | |
Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000201011&lng=en&tlng=en |
id |
doaj-38224da9c05045aeaa326add0e77d51a |
---|---|
record_format |
Article |
spelling |
doaj-38224da9c05045aeaa326add0e77d51a2020-11-25T03:28:59ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências0001-37651678-269092suppl 110.1590/0001-3765202020180477S0001-37652020000201011Inhibition kinetics of digestive proteases for Anticarsia gemmatalisADRIANA M. PATARROYO-VARGASGLÁUCIA CORDEIROCAROLINA R. DA SILVACAMILA R. DA SILVAEDUARDO G. MENDONÇALILIANE E. VISÔTTOJOSÉ C. ZANUNCIOWELLIGTON G. CAMPOSMARIA GORETI A. OLIVEIRAAbstract Anticarsia gemmatalis Hübner, 1818 (Lepidoptera) is a major pest of soybean in the Brazil. It is known that the reduction of proteolytic activity by the ingestion of protease inhibitors reduces digestion and larval development of the insects. Control via inhibition of the digestive enzymes necessitates deeper knowledge of the enzyme kinetics and the characterization of the inhibition kinetics of these proteases, for better understanding of the active centers and action mechanisms of this enzyme. Trypsin-like proteases found in the gut of Anticarsia gemmatalis were purified in a p-aminobenzamidine agarose column. Kinetic characterization showed KM 0.503 mM for the L-BApNA substrate; Vmax= 46.650 nM s-1; Vmax/[E]= 9.256 nM s-1 mg L-1 and Vmax/[E]/KM= 18.402 nM s-1 mg L-1 mM. The Ki values for the inhibitors benzamidine, berenil, SKTI and SBBI were 11.2 µM, 32.4 µM, 0.25 nM and 1.4 nM, respectively, and all revealed linear competitive inhibition. The SKTI showed the greatest inhibition, which makes it a promising subject for future research to manufacture peptide mimetic inhibitors.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000201011&lng=en&tlng=enenzyme kineticsinhibitor competitiveinhibition kineticsvelvet bean caterpillar |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
ADRIANA M. PATARROYO-VARGAS GLÁUCIA CORDEIRO CAROLINA R. DA SILVA CAMILA R. DA SILVA EDUARDO G. MENDONÇA LILIANE E. VISÔTTO JOSÉ C. ZANUNCIO WELLIGTON G. CAMPOS MARIA GORETI A. OLIVEIRA |
spellingShingle |
ADRIANA M. PATARROYO-VARGAS GLÁUCIA CORDEIRO CAROLINA R. DA SILVA CAMILA R. DA SILVA EDUARDO G. MENDONÇA LILIANE E. VISÔTTO JOSÉ C. ZANUNCIO WELLIGTON G. CAMPOS MARIA GORETI A. OLIVEIRA Inhibition kinetics of digestive proteases for Anticarsia gemmatalis Anais da Academia Brasileira de Ciências enzyme kinetics inhibitor competitive inhibition kinetics velvet bean caterpillar |
author_facet |
ADRIANA M. PATARROYO-VARGAS GLÁUCIA CORDEIRO CAROLINA R. DA SILVA CAMILA R. DA SILVA EDUARDO G. MENDONÇA LILIANE E. VISÔTTO JOSÉ C. ZANUNCIO WELLIGTON G. CAMPOS MARIA GORETI A. OLIVEIRA |
author_sort |
ADRIANA M. PATARROYO-VARGAS |
title |
Inhibition kinetics of digestive proteases for Anticarsia gemmatalis |
title_short |
Inhibition kinetics of digestive proteases for Anticarsia gemmatalis |
title_full |
Inhibition kinetics of digestive proteases for Anticarsia gemmatalis |
title_fullStr |
Inhibition kinetics of digestive proteases for Anticarsia gemmatalis |
title_full_unstemmed |
Inhibition kinetics of digestive proteases for Anticarsia gemmatalis |
title_sort |
inhibition kinetics of digestive proteases for anticarsia gemmatalis |
publisher |
Academia Brasileira de Ciências |
series |
Anais da Academia Brasileira de Ciências |
issn |
0001-3765 1678-2690 |
description |
Abstract Anticarsia gemmatalis Hübner, 1818 (Lepidoptera) is a major pest of soybean in the Brazil. It is known that the reduction of proteolytic activity by the ingestion of protease inhibitors reduces digestion and larval development of the insects. Control via inhibition of the digestive enzymes necessitates deeper knowledge of the enzyme kinetics and the characterization of the inhibition kinetics of these proteases, for better understanding of the active centers and action mechanisms of this enzyme. Trypsin-like proteases found in the gut of Anticarsia gemmatalis were purified in a p-aminobenzamidine agarose column. Kinetic characterization showed KM 0.503 mM for the L-BApNA substrate; Vmax= 46.650 nM s-1; Vmax/[E]= 9.256 nM s-1 mg L-1 and Vmax/[E]/KM= 18.402 nM s-1 mg L-1 mM. The Ki values for the inhibitors benzamidine, berenil, SKTI and SBBI were 11.2 µM, 32.4 µM, 0.25 nM and 1.4 nM, respectively, and all revealed linear competitive inhibition. The SKTI showed the greatest inhibition, which makes it a promising subject for future research to manufacture peptide mimetic inhibitors. |
topic |
enzyme kinetics inhibitor competitive inhibition kinetics velvet bean caterpillar |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000201011&lng=en&tlng=en |
work_keys_str_mv |
AT adrianampatarroyovargas inhibitionkineticsofdigestiveproteasesforanticarsiagemmatalis AT glauciacordeiro inhibitionkineticsofdigestiveproteasesforanticarsiagemmatalis AT carolinardasilva inhibitionkineticsofdigestiveproteasesforanticarsiagemmatalis AT camilardasilva inhibitionkineticsofdigestiveproteasesforanticarsiagemmatalis AT eduardogmendonca inhibitionkineticsofdigestiveproteasesforanticarsiagemmatalis AT lilianeevisotto inhibitionkineticsofdigestiveproteasesforanticarsiagemmatalis AT joseczanuncio inhibitionkineticsofdigestiveproteasesforanticarsiagemmatalis AT welligtongcampos inhibitionkineticsofdigestiveproteasesforanticarsiagemmatalis AT mariagoretiaoliveira inhibitionkineticsofdigestiveproteasesforanticarsiagemmatalis |
_version_ |
1724581515790123008 |