Proanthocyanidins from Grape Seeds Modulate the NF-κB Signal Transduction Pathways in Rats with TNBS-Induced Ulcerative Colitis

• Main Authors: Yongjie Wu, Sijia Li, Jiajia Chen, Xin Zhao, Liping Wang, Shuai Yan, Xiaoxia Wang, Yanhui Huang, Yanhong Wang, Li Wang, Xiaoli Li, Xiaolai Yang, Yongqing Cai, Hong Qin, Wan Li
• Format: Article
• Language: English
• Published: MDPI AG 2011-08-01
• Series: Molecules
• Subjects: proanthocyanidins from grape seeds; ulcerative colitis; nuclear factor-kappa B
• Online Access: http://www.mdpi.com/1420-3049/16/8/6721/

To elucidate the molecular mechanisms involved in the therapeutic effects of proanthocyanidins from grape seeds (GSPE), we explore whether GSPE regulates the inflammatory response of TNBS-induced colitis in rats at the levels of NF-κB signal transduction pathway. Rats were intragastrically administered of different doses of GSPE (100, 200 and 400 mg·kg−1) per day for seven days after ulcerative colitis (UC) was induced by intracolonic injection of 2,4,6-trinitrobenzenesulfonic acid (TNBS) dissolved in 50% ethanol. Sulfasalazine (SASP) at 400 mg/kg was used as a positive control drug. The expression of nuclear factor-kappa B (NF-κB), phospho-I kappaB-alpha (pIκBα), inhibitor kappa B kinase (IκK) in the colon tissues were all measured by enzyme-linked immunosorbent assay (ELISA) methods. Treatment with GSPE reduced the expression of NF-κB, pIκBα and IκK in the colon. The results of this study show that GSPE exerts beneficial effects in inflammatory bowel disease by inhibition of NF-κB signal transduction pathways.