Microfluidic Method of Pig Oocyte Quality Assessment in relation to Different Follicular Size Based on Lab-on-Chip Technology
Since microfollicular environment and the size of the follicle are important markers influencing oocyte quality, the aim of this study is to present the spectral characterization of oocytes isolated from follicles of various sizes using lab-on-chip (LOC) technology and to demonstrate how follicle si...
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doaj-383814e777ec480f8a9ee8f641ce346b2020-11-24T23:19:54ZengHindawi LimitedBioMed Research International2314-61332314-61412014-01-01201410.1155/2014/467063467063Microfluidic Method of Pig Oocyte Quality Assessment in relation to Different Follicular Size Based on Lab-on-Chip TechnologyBartosz Kempisty0Rafał Walczak1Paweł Antosik2Patrycja Sniadek3Marta Rybska4Hanna Piotrowska5Dorota Bukowska6Jan Dziuban7Michał Nowicki8Jędrzej M. Jaśkowski9Maciej Zabel10Klaus-Peter Brüssow11Department of Histology and Embryology, Poznan University of Medical Sciences, 6 Swiecickiego Street, 60-781 Poznan, PolandDepartment of Microengineering and Photovoltaics, Wroclaw University of Technology, 11/17 Janiszewskiego Street, 50-372 Wroclaw, PolandInstitute of Veterinary Sciences, Poznan University of Life Sciences, 52 Wojska Polskiego Street, 60-628 Poznan, PolandDepartment of Microengineering and Photovoltaics, Wroclaw University of Technology, 11/17 Janiszewskiego Street, 50-372 Wroclaw, PolandInstitute of Veterinary Sciences, Poznan University of Life Sciences, 52 Wojska Polskiego Street, 60-628 Poznan, PolandDepartment of Toxicology, Poznan University of Medical Sciences, 30 Dojazd Street, 60-631 Poznan, PolandInstitute of Veterinary Sciences, Poznan University of Life Sciences, 52 Wojska Polskiego Street, 60-628 Poznan, PolandDepartment of Anatomy, Poznan University of Medical Science, 6 Swiecickiego Street, 60-781 Poznan, PolandDepartment of Histology and Embryology, Poznan University of Medical Sciences, 6 Swiecickiego Street, 60-781 Poznan, PolandInstitute of Veterinary Sciences, Poznan University of Life Sciences, 52 Wojska Polskiego Street, 60-628 Poznan, PolandDepartment of Histology and Embryology, Wroclaw Medical University, 6a Chalubinskiego Street, 50-368 Wroclaw, PolandDepartment of Experimental Reproductive Biology, Institute of Reproductive Biology, Leibniz Institute for Farm Animal Biology, Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, GermanySince microfollicular environment and the size of the follicle are important markers influencing oocyte quality, the aim of this study is to present the spectral characterization of oocytes isolated from follicles of various sizes using lab-on-chip (LOC) technology and to demonstrate how follicle size may affect oocyte quality. Porcine oocytes (each, n=100) recovered from follicles of different sizes, for example, from large (>5 mm), medium (3–5 mm), and small (<3 mm), were analyzed after preceding in vitro maturation (IVM). The LOC analysis was performed using a silicon-glass sandwich with two glass optical fibers positioned “face-to-face.” Oocytes collected from follicles of different size classes revealed specific and distinguishable spectral characteristics. The absorbance spectra (microspectrometric specificity) for oocytes isolated from large, medium, and small follicles differ significantly (P<0.05) and the absorbance wavelengths were between 626 and 628 nm, between 618 and 620 nm, and less than 618 nm, respectively. The present study offers a parametric and objective method of porcine oocyte assessment. However, up to now this study has been used to evidence spectral markers associated with follicular size in pigs, only. Further investigations with functional-biological assays and comparing LOC analyses with fertilization and pregnancy success and the outcome of healthy offspring must be performed.http://dx.doi.org/10.1155/2014/467063 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Bartosz Kempisty Rafał Walczak Paweł Antosik Patrycja Sniadek Marta Rybska Hanna Piotrowska Dorota Bukowska Jan Dziuban Michał Nowicki Jędrzej M. Jaśkowski Maciej Zabel Klaus-Peter Brüssow |
spellingShingle |
Bartosz Kempisty Rafał Walczak Paweł Antosik Patrycja Sniadek Marta Rybska Hanna Piotrowska Dorota Bukowska Jan Dziuban Michał Nowicki Jędrzej M. Jaśkowski Maciej Zabel Klaus-Peter Brüssow Microfluidic Method of Pig Oocyte Quality Assessment in relation to Different Follicular Size Based on Lab-on-Chip Technology BioMed Research International |
author_facet |
Bartosz Kempisty Rafał Walczak Paweł Antosik Patrycja Sniadek Marta Rybska Hanna Piotrowska Dorota Bukowska Jan Dziuban Michał Nowicki Jędrzej M. Jaśkowski Maciej Zabel Klaus-Peter Brüssow |
author_sort |
Bartosz Kempisty |
title |
Microfluidic Method of Pig Oocyte Quality Assessment in relation to Different Follicular Size Based on Lab-on-Chip Technology |
title_short |
Microfluidic Method of Pig Oocyte Quality Assessment in relation to Different Follicular Size Based on Lab-on-Chip Technology |
title_full |
Microfluidic Method of Pig Oocyte Quality Assessment in relation to Different Follicular Size Based on Lab-on-Chip Technology |
title_fullStr |
Microfluidic Method of Pig Oocyte Quality Assessment in relation to Different Follicular Size Based on Lab-on-Chip Technology |
title_full_unstemmed |
Microfluidic Method of Pig Oocyte Quality Assessment in relation to Different Follicular Size Based on Lab-on-Chip Technology |
title_sort |
microfluidic method of pig oocyte quality assessment in relation to different follicular size based on lab-on-chip technology |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2014-01-01 |
description |
Since microfollicular environment and the size of the follicle are important markers influencing oocyte quality, the aim of this study is to present the spectral characterization of oocytes isolated from follicles of various sizes using lab-on-chip (LOC) technology and to demonstrate how follicle size may affect oocyte quality. Porcine oocytes (each, n=100) recovered from follicles of different sizes, for example, from large (>5 mm), medium (3–5 mm), and small (<3 mm), were analyzed after preceding in vitro maturation (IVM). The LOC analysis was performed using a silicon-glass sandwich with two glass optical fibers positioned “face-to-face.” Oocytes collected from follicles of different size classes revealed specific and distinguishable spectral characteristics. The absorbance spectra (microspectrometric specificity) for oocytes isolated from large, medium, and small follicles differ significantly (P<0.05) and the absorbance wavelengths were between 626 and 628 nm, between 618 and 620 nm, and less than 618 nm, respectively. The present study offers a parametric and objective method of porcine oocyte assessment. However, up to now this study has been used to evidence spectral markers associated with follicular size in pigs, only. Further investigations with functional-biological assays and comparing LOC analyses with fertilization and pregnancy success and the outcome of healthy offspring must be performed. |
url |
http://dx.doi.org/10.1155/2014/467063 |
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