Transcriptome Analysis Reveals Impaired Fertility and Immunity Under Salinity Exposure in Juvenile Grass Carp
Grass carp (Ctenopharyngodon idellus) is one of the most economically important aquaculture species and is widely cultured in China. However, its wild populations in many rivers are increasingly declining, and seawater intrusion is one of the most important threats to their survival. However, the me...
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Language: | English |
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Frontiers Media S.A.
2021-08-01
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Series: | Frontiers in Marine Science |
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Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2021.697813/full |
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doaj-38455f287f214fb98f2f63c6c74136a0 |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jingjing Zhang Jingjing Zhang Jingjing Zhang Jingjing Zhang Zhi Wu Zhi Wu Zhi Wu Zhi Wu Yujie He Yujie He Yujie He Yujie He Xinhui Li Xinhui Li Xinhui Li Xinhui Li Jie Li Jie Li Jie Li Jie Li |
spellingShingle |
Jingjing Zhang Jingjing Zhang Jingjing Zhang Jingjing Zhang Zhi Wu Zhi Wu Zhi Wu Zhi Wu Yujie He Yujie He Yujie He Yujie He Xinhui Li Xinhui Li Xinhui Li Xinhui Li Jie Li Jie Li Jie Li Jie Li Transcriptome Analysis Reveals Impaired Fertility and Immunity Under Salinity Exposure in Juvenile Grass Carp Frontiers in Marine Science larval grass carp RNA-seq fertility immunity seawater intrusion |
author_facet |
Jingjing Zhang Jingjing Zhang Jingjing Zhang Jingjing Zhang Zhi Wu Zhi Wu Zhi Wu Zhi Wu Yujie He Yujie He Yujie He Yujie He Xinhui Li Xinhui Li Xinhui Li Xinhui Li Jie Li Jie Li Jie Li Jie Li |
author_sort |
Jingjing Zhang |
title |
Transcriptome Analysis Reveals Impaired Fertility and Immunity Under Salinity Exposure in Juvenile Grass Carp |
title_short |
Transcriptome Analysis Reveals Impaired Fertility and Immunity Under Salinity Exposure in Juvenile Grass Carp |
title_full |
Transcriptome Analysis Reveals Impaired Fertility and Immunity Under Salinity Exposure in Juvenile Grass Carp |
title_fullStr |
Transcriptome Analysis Reveals Impaired Fertility and Immunity Under Salinity Exposure in Juvenile Grass Carp |
title_full_unstemmed |
Transcriptome Analysis Reveals Impaired Fertility and Immunity Under Salinity Exposure in Juvenile Grass Carp |
title_sort |
transcriptome analysis reveals impaired fertility and immunity under salinity exposure in juvenile grass carp |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Marine Science |
issn |
2296-7745 |
publishDate |
2021-08-01 |
description |
Grass carp (Ctenopharyngodon idellus) is one of the most economically important aquaculture species and is widely cultured in China. However, its wild populations in many rivers are increasingly declining, and seawater intrusion is one of the most important threats to their survival. However, the mechanisms underlying the decline due to salinity pressure are still unknown. Here, we performed a comparative transcriptome analysis of C. idellus larvae in response to salinity exposures; a total of 481 differentially expressed genes (DEGs) were identified. These DEGs were significantly enriched in eight Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, among which steroid biosynthesis was the most important one, with the highest enrichment score. The pathway plays an important role in the development of the testes and ovary. Interestingly, all DEGs in steroid biosynthesis showed a down regulation, indicating that salinity exposure may pose damage to the fertility of C. idellus. Furthermore, three immunity-associated pathways (cytokine–cytokine receptor interaction, Toll-like receptor signaling pathway, and NOD-like receptor signaling pathway) were also significantly enriched, suggesting impaired immunity and a high risk of disease infection under salinity exposure. Overall, damage to both fertility and immunity would decrease the number of offspring and increase the risk of death due to disease infection. Our results provide a potential molecular mechanism underlying the decline of wild C. idellus populations in the Pearl River. |
topic |
larval grass carp RNA-seq fertility immunity seawater intrusion |
url |
https://www.frontiersin.org/articles/10.3389/fmars.2021.697813/full |
work_keys_str_mv |
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doaj-38455f287f214fb98f2f63c6c74136a02021-08-13T16:06:22ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452021-08-01810.3389/fmars.2021.697813697813Transcriptome Analysis Reveals Impaired Fertility and Immunity Under Salinity Exposure in Juvenile Grass CarpJingjing Zhang0Jingjing Zhang1Jingjing Zhang2Jingjing Zhang3Zhi Wu4Zhi Wu5Zhi Wu6Zhi Wu7Yujie He8Yujie He9Yujie He10Yujie He11Xinhui Li12Xinhui Li13Xinhui Li14Xinhui Li15Jie Li16Jie Li17Jie Li18Jie Li19Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaGuangzhou Scientific Observing and Experimental Station of National Fisheries Resources and Environment, Guangzhou, ChinaScientific Observing and Experimental Station of Fishery Resources and Environment in the Middle and Lower Reaches of Pearl River, Ministry of Agriculture and Rural Affairs, Guangzhou, ChinaKey Laboratory of Aquatic Animal Immune Technology of Guangdong Province, Guangzhou, ChinaPearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaGuangzhou Scientific Observing and Experimental Station of National Fisheries Resources and Environment, Guangzhou, ChinaScientific Observing and Experimental Station of Fishery Resources and Environment in the Middle and Lower Reaches of Pearl River, Ministry of Agriculture and Rural Affairs, Guangzhou, ChinaKey Laboratory of Aquatic Animal Immune Technology of Guangdong Province, Guangzhou, ChinaPearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaGuangzhou Scientific Observing and Experimental Station of National Fisheries Resources and Environment, Guangzhou, ChinaScientific Observing and Experimental Station of Fishery Resources and Environment in the Middle and Lower Reaches of Pearl River, Ministry of Agriculture and Rural Affairs, Guangzhou, ChinaKey Laboratory of Aquatic Animal Immune Technology of Guangdong Province, Guangzhou, ChinaPearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaGuangzhou Scientific Observing and Experimental Station of National Fisheries Resources and Environment, Guangzhou, ChinaScientific Observing and Experimental Station of Fishery Resources and Environment in the Middle and Lower Reaches of Pearl River, Ministry of Agriculture and Rural Affairs, Guangzhou, ChinaKey Laboratory of Aquatic Animal Immune Technology of Guangdong Province, Guangzhou, ChinaPearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaGuangzhou Scientific Observing and Experimental Station of National Fisheries Resources and Environment, Guangzhou, ChinaScientific Observing and Experimental Station of Fishery Resources and Environment in the Middle and Lower Reaches of Pearl River, Ministry of Agriculture and Rural Affairs, Guangzhou, ChinaKey Laboratory of Aquatic Animal Immune Technology of Guangdong Province, Guangzhou, ChinaGrass carp (Ctenopharyngodon idellus) is one of the most economically important aquaculture species and is widely cultured in China. However, its wild populations in many rivers are increasingly declining, and seawater intrusion is one of the most important threats to their survival. However, the mechanisms underlying the decline due to salinity pressure are still unknown. Here, we performed a comparative transcriptome analysis of C. idellus larvae in response to salinity exposures; a total of 481 differentially expressed genes (DEGs) were identified. These DEGs were significantly enriched in eight Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, among which steroid biosynthesis was the most important one, with the highest enrichment score. The pathway plays an important role in the development of the testes and ovary. Interestingly, all DEGs in steroid biosynthesis showed a down regulation, indicating that salinity exposure may pose damage to the fertility of C. idellus. Furthermore, three immunity-associated pathways (cytokine–cytokine receptor interaction, Toll-like receptor signaling pathway, and NOD-like receptor signaling pathway) were also significantly enriched, suggesting impaired immunity and a high risk of disease infection under salinity exposure. Overall, damage to both fertility and immunity would decrease the number of offspring and increase the risk of death due to disease infection. Our results provide a potential molecular mechanism underlying the decline of wild C. idellus populations in the Pearl River.https://www.frontiersin.org/articles/10.3389/fmars.2021.697813/fulllarval grass carpRNA-seqfertilityimmunityseawater intrusion |