Cell penetration efficiency analysis of different atomic force microscopy nanoneedles into living cells
Abstract Over the last decade, nanoneedle-based systems have demonstrated to be extremely useful in cell biology. They can be used as nanotools for drug delivery, biosensing or biomolecular recognition inside cells; or they can be employed to select and sort in parallel a large number of living cell...
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2021-04-01
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Online Access: | https://doi.org/10.1038/s41598-021-87319-3 |
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doaj-384d53bd750d40939873a02a0ac551262021-04-11T11:32:32ZengNature Publishing GroupScientific Reports2045-23222021-04-011111810.1038/s41598-021-87319-3Cell penetration efficiency analysis of different atomic force microscopy nanoneedles into living cellsMarcos Penedo0Tetsuya Shirokawa1Mohammad Shahidul Alam2Keisuke Miyazawa3Takehiko Ichikawa4Naoko Okano5Hirotoshi Furusho6Chikashi Nakamura7Takeshi Fukuma8Nano Life Science Institute (WPI-NanoLSI), Kanazawa UniversityDivision of Electrical Engineering and Computer Science, Kanazawa UniversityDivision of Nano Life Science, Kanazawa UniversityNano Life Science Institute (WPI-NanoLSI), Kanazawa UniversityNano Life Science Institute (WPI-NanoLSI), Kanazawa UniversityNano Life Science Institute (WPI-NanoLSI), Kanazawa UniversityNano Life Science Institute (WPI-NanoLSI), Kanazawa UniversityAIST-INDIA Diverse Assets and Applications International Laboratory (DAILAB), Cellular and Molecular Biotechnology Research Institute (CMB), National Institute of Advanced Industrial Science and Technology (AIST)Nano Life Science Institute (WPI-NanoLSI), Kanazawa UniversityAbstract Over the last decade, nanoneedle-based systems have demonstrated to be extremely useful in cell biology. They can be used as nanotools for drug delivery, biosensing or biomolecular recognition inside cells; or they can be employed to select and sort in parallel a large number of living cells. When using these nanoprobes, the most important requirement is to minimize the cell damage, reducing the forces and indentation lengths needed to penetrate the cell membrane. This is normally achieved by reducing the diameter of the nanoneedles. However, several studies have shown that nanoneedles with a flat tip display lower penetration forces and indentation lengths. In this work, we have tested different nanoneedle shapes and diameters to reduce the force and the indentation length needed to penetrate the cell membrane, demonstrating that ultra-thin and sharp nanoprobes can further reduce them, consequently minimizing the cell damage.https://doi.org/10.1038/s41598-021-87319-3 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Marcos Penedo Tetsuya Shirokawa Mohammad Shahidul Alam Keisuke Miyazawa Takehiko Ichikawa Naoko Okano Hirotoshi Furusho Chikashi Nakamura Takeshi Fukuma |
spellingShingle |
Marcos Penedo Tetsuya Shirokawa Mohammad Shahidul Alam Keisuke Miyazawa Takehiko Ichikawa Naoko Okano Hirotoshi Furusho Chikashi Nakamura Takeshi Fukuma Cell penetration efficiency analysis of different atomic force microscopy nanoneedles into living cells Scientific Reports |
author_facet |
Marcos Penedo Tetsuya Shirokawa Mohammad Shahidul Alam Keisuke Miyazawa Takehiko Ichikawa Naoko Okano Hirotoshi Furusho Chikashi Nakamura Takeshi Fukuma |
author_sort |
Marcos Penedo |
title |
Cell penetration efficiency analysis of different atomic force microscopy nanoneedles into living cells |
title_short |
Cell penetration efficiency analysis of different atomic force microscopy nanoneedles into living cells |
title_full |
Cell penetration efficiency analysis of different atomic force microscopy nanoneedles into living cells |
title_fullStr |
Cell penetration efficiency analysis of different atomic force microscopy nanoneedles into living cells |
title_full_unstemmed |
Cell penetration efficiency analysis of different atomic force microscopy nanoneedles into living cells |
title_sort |
cell penetration efficiency analysis of different atomic force microscopy nanoneedles into living cells |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2021-04-01 |
description |
Abstract Over the last decade, nanoneedle-based systems have demonstrated to be extremely useful in cell biology. They can be used as nanotools for drug delivery, biosensing or biomolecular recognition inside cells; or they can be employed to select and sort in parallel a large number of living cells. When using these nanoprobes, the most important requirement is to minimize the cell damage, reducing the forces and indentation lengths needed to penetrate the cell membrane. This is normally achieved by reducing the diameter of the nanoneedles. However, several studies have shown that nanoneedles with a flat tip display lower penetration forces and indentation lengths. In this work, we have tested different nanoneedle shapes and diameters to reduce the force and the indentation length needed to penetrate the cell membrane, demonstrating that ultra-thin and sharp nanoprobes can further reduce them, consequently minimizing the cell damage. |
url |
https://doi.org/10.1038/s41598-021-87319-3 |
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