Poliovirus 2A protease triggers a selective nucleo-cytoplasmic redistribution of splicing factors to regulate alternative pre-mRNA splicing.

Poliovirus 2A protease triggers a selective nucleo-cytoplasmic redistribution of splicing factors to regulate alternative pre-mRNA splicing.

Poliovirus protease 2A (2A(pro)) obstructs host gene expression by reprogramming transcriptional and post-transcriptional regulatory events during infection. Here we demonstrate that expression of 2A(pro) induces a selective nucleo-cytoplasm translocation of several important RNA binding proteins an...

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Main Authors: Enrique Álvarez, Alfredo Castelló, Luis Carrasco, José M Izquierdo
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3774746?pdf=render
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spelling doaj-387285fad23c43fda3c4bd290de875862020-11-25T01:56:02ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0189e7372310.1371/journal.pone.0073723Poliovirus 2A protease triggers a selective nucleo-cytoplasmic redistribution of splicing factors to regulate alternative pre-mRNA splicing.Enrique ÁlvarezAlfredo CastellóLuis CarrascoJosé M IzquierdoPoliovirus protease 2A (2A(pro)) obstructs host gene expression by reprogramming transcriptional and post-transcriptional regulatory events during infection. Here we demonstrate that expression of 2A(pro) induces a selective nucleo-cytoplasm translocation of several important RNA binding proteins and splicing factors. Subcellular fractionation studies, together with immunofluorescence microscopy revealed an asymmetric distribution of HuR and TIA1/TIAR in 2A(pro) expressing cells, which modulates splicing of the human Fas exon 6. Consistent with this result, knockdown of HuR or overexpression of TIA1/TIAR, leads to Fas exon 6 inclusion in 2A(pro)-expressing cells. Therefore, poliovirus 2A(pro) can target alternative pre-mRNA splicing by regulating protein shuttling between the nucleus and the cytoplasm.http://europepmc.org/articles/PMC3774746?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Enrique Álvarez
Alfredo Castelló
Luis Carrasco
José M Izquierdo
spellingShingle Enrique Álvarez
Alfredo Castelló
Luis Carrasco
José M Izquierdo
Poliovirus 2A protease triggers a selective nucleo-cytoplasmic redistribution of splicing factors to regulate alternative pre-mRNA splicing.
PLoS ONE
author_facet Enrique Álvarez
Alfredo Castelló
Luis Carrasco
José M Izquierdo
author_sort Enrique Álvarez
title Poliovirus 2A protease triggers a selective nucleo-cytoplasmic redistribution of splicing factors to regulate alternative pre-mRNA splicing.
title_short Poliovirus 2A protease triggers a selective nucleo-cytoplasmic redistribution of splicing factors to regulate alternative pre-mRNA splicing.
title_full Poliovirus 2A protease triggers a selective nucleo-cytoplasmic redistribution of splicing factors to regulate alternative pre-mRNA splicing.
title_fullStr Poliovirus 2A protease triggers a selective nucleo-cytoplasmic redistribution of splicing factors to regulate alternative pre-mRNA splicing.
title_full_unstemmed Poliovirus 2A protease triggers a selective nucleo-cytoplasmic redistribution of splicing factors to regulate alternative pre-mRNA splicing.
title_sort poliovirus 2a protease triggers a selective nucleo-cytoplasmic redistribution of splicing factors to regulate alternative pre-mrna splicing.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2013-01-01
description Poliovirus protease 2A (2A(pro)) obstructs host gene expression by reprogramming transcriptional and post-transcriptional regulatory events during infection. Here we demonstrate that expression of 2A(pro) induces a selective nucleo-cytoplasm translocation of several important RNA binding proteins and splicing factors. Subcellular fractionation studies, together with immunofluorescence microscopy revealed an asymmetric distribution of HuR and TIA1/TIAR in 2A(pro) expressing cells, which modulates splicing of the human Fas exon 6. Consistent with this result, knockdown of HuR or overexpression of TIA1/TIAR, leads to Fas exon 6 inclusion in 2A(pro)-expressing cells. Therefore, poliovirus 2A(pro) can target alternative pre-mRNA splicing by regulating protein shuttling between the nucleus and the cytoplasm.
url http://europepmc.org/articles/PMC3774746?pdf=render
work_keys_str_mv AT enriquealvarez poliovirus2aproteasetriggersaselectivenucleocytoplasmicredistributionofsplicingfactorstoregulatealternativepremrnasplicing
AT alfredocastello poliovirus2aproteasetriggersaselectivenucleocytoplasmicredistributionofsplicingfactorstoregulatealternativepremrnasplicing
AT luiscarrasco poliovirus2aproteasetriggersaselectivenucleocytoplasmicredistributionofsplicingfactorstoregulatealternativepremrnasplicing
AT josemizquierdo poliovirus2aproteasetriggersaselectivenucleocytoplasmicredistributionofsplicingfactorstoregulatealternativepremrnasplicing
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