Quantitative Trait Locus Mapping of Flowering Time and Maturity in Soybean Using Next-Generation Sequencing-Based Analysis
Soybean (Glycine max L.) is a major legume crop that is mainly distributed in temperate regions. The adaptability of soybean to grow at relatively high latitudes is attributed to natural variations in major genes and quantitative trait loci (QTLs) that control flowering time and maturity. Identifica...
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Format: | Article |
Language: | English |
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Frontiers Media S.A.
2018-07-01
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Series: | Frontiers in Plant Science |
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Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2018.00995/full |
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doaj-38b37aba81fe4838931b198f3f85156e |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lingping Kong Lingping Kong Sijia Lu Yanping Wang Chao Fang Chao Fang Feifei Wang Haiyang Nan Tong Su Tong Su Shichen Li Shichen Li Fengge Zhang Fengge Zhang Xiaoming Li Xiaohui Zhao Xiaohui Yuan Baohui Liu Baohui Liu Fanjiang Kong Fanjiang Kong |
spellingShingle |
Lingping Kong Lingping Kong Sijia Lu Yanping Wang Chao Fang Chao Fang Feifei Wang Haiyang Nan Tong Su Tong Su Shichen Li Shichen Li Fengge Zhang Fengge Zhang Xiaoming Li Xiaohui Zhao Xiaohui Yuan Baohui Liu Baohui Liu Fanjiang Kong Fanjiang Kong Quantitative Trait Locus Mapping of Flowering Time and Maturity in Soybean Using Next-Generation Sequencing-Based Analysis Frontiers in Plant Science soybean flowering time maturity reproduction period quantitative trait loci |
author_facet |
Lingping Kong Lingping Kong Sijia Lu Yanping Wang Chao Fang Chao Fang Feifei Wang Haiyang Nan Tong Su Tong Su Shichen Li Shichen Li Fengge Zhang Fengge Zhang Xiaoming Li Xiaohui Zhao Xiaohui Yuan Baohui Liu Baohui Liu Fanjiang Kong Fanjiang Kong |
author_sort |
Lingping Kong |
title |
Quantitative Trait Locus Mapping of Flowering Time and Maturity in Soybean Using Next-Generation Sequencing-Based Analysis |
title_short |
Quantitative Trait Locus Mapping of Flowering Time and Maturity in Soybean Using Next-Generation Sequencing-Based Analysis |
title_full |
Quantitative Trait Locus Mapping of Flowering Time and Maturity in Soybean Using Next-Generation Sequencing-Based Analysis |
title_fullStr |
Quantitative Trait Locus Mapping of Flowering Time and Maturity in Soybean Using Next-Generation Sequencing-Based Analysis |
title_full_unstemmed |
Quantitative Trait Locus Mapping of Flowering Time and Maturity in Soybean Using Next-Generation Sequencing-Based Analysis |
title_sort |
quantitative trait locus mapping of flowering time and maturity in soybean using next-generation sequencing-based analysis |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2018-07-01 |
description |
Soybean (Glycine max L.) is a major legume crop that is mainly distributed in temperate regions. The adaptability of soybean to grow at relatively high latitudes is attributed to natural variations in major genes and quantitative trait loci (QTLs) that control flowering time and maturity. Identification of new QTLs and map-based cloning of candidate genes are the fundamental approaches in elucidating the mechanism underlying soybean flowering and adaptation. To identify novel QTLs/genes, we developed two F8:10 recombinant inbred lines (RILs) and evaluated the traits of time to flowering (R1), maturity (R8), and reproductive period (RP) in the field. To rapidly and efficiently identify QTLs that control these traits, next-generation sequencing (NGS)-based QTL analysis was performed. This study demonstrates that only one major QTL on chromosome 4 simultaneously controls R1, R8, and RP traits in the Dongnong 50 × Williams 82 (DW) RIL population. Furthermore, three QTLs were mapped to chromosomes 6, 11, and 16 in the Suinong 14 × Enrei (SE) RIL population. Two major pleiotropic QTLs on chromosomes 4 and 6 were shown to affect flowering time, maturity, and RP. A QTL influencing RP was identified on chromosome 11, and QTL on chromosome 16 was associated with time to flowering responses. All these QTLs contributed to soybean maturation. The QTLs identified in this study may be utilized in fine mapping and map-based cloning of candidate genes to elucidate the mechanisms underlying flowering and soybean adaptation to different latitudes and to breed novel soybean cultivars with optimal yield-related traits. |
topic |
soybean flowering time maturity reproduction period quantitative trait loci |
url |
https://www.frontiersin.org/article/10.3389/fpls.2018.00995/full |
work_keys_str_mv |
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doaj-38b37aba81fe4838931b198f3f85156e2020-11-24T21:15:29ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2018-07-01910.3389/fpls.2018.00995339476Quantitative Trait Locus Mapping of Flowering Time and Maturity in Soybean Using Next-Generation Sequencing-Based AnalysisLingping Kong0Lingping Kong1Sijia Lu2Yanping Wang3Chao Fang4Chao Fang5Feifei Wang6Haiyang Nan7Tong Su8Tong Su9Shichen Li10Shichen Li11Fengge Zhang12Fengge Zhang13Xiaoming Li14Xiaohui Zhao15Xiaohui Yuan16Baohui Liu17Baohui Liu18Fanjiang Kong19Fanjiang Kong20School of Life Sciences, Guangzhou University, Guangzhou, ChinaThe Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, ChinaSchool of Life Sciences, Guangzhou University, Guangzhou, ChinaMudanjiang Branch of Heilongjiang Academy of Agricultural Sciences, Mudanjiang, ChinaThe Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaThe Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, ChinaSchool of Life Sciences, Guangzhou University, Guangzhou, ChinaThe Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaThe Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaThe Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaThe Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, ChinaSchool of Life Sciences, Guangzhou University, Guangzhou, ChinaThe Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, ChinaSchool of Life Sciences, Guangzhou University, Guangzhou, ChinaThe Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, ChinaSchool of Life Sciences, Guangzhou University, Guangzhou, ChinaThe Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, ChinaSoybean (Glycine max L.) is a major legume crop that is mainly distributed in temperate regions. The adaptability of soybean to grow at relatively high latitudes is attributed to natural variations in major genes and quantitative trait loci (QTLs) that control flowering time and maturity. Identification of new QTLs and map-based cloning of candidate genes are the fundamental approaches in elucidating the mechanism underlying soybean flowering and adaptation. To identify novel QTLs/genes, we developed two F8:10 recombinant inbred lines (RILs) and evaluated the traits of time to flowering (R1), maturity (R8), and reproductive period (RP) in the field. To rapidly and efficiently identify QTLs that control these traits, next-generation sequencing (NGS)-based QTL analysis was performed. This study demonstrates that only one major QTL on chromosome 4 simultaneously controls R1, R8, and RP traits in the Dongnong 50 × Williams 82 (DW) RIL population. Furthermore, three QTLs were mapped to chromosomes 6, 11, and 16 in the Suinong 14 × Enrei (SE) RIL population. Two major pleiotropic QTLs on chromosomes 4 and 6 were shown to affect flowering time, maturity, and RP. A QTL influencing RP was identified on chromosome 11, and QTL on chromosome 16 was associated with time to flowering responses. All these QTLs contributed to soybean maturation. The QTLs identified in this study may be utilized in fine mapping and map-based cloning of candidate genes to elucidate the mechanisms underlying flowering and soybean adaptation to different latitudes and to breed novel soybean cultivars with optimal yield-related traits.https://www.frontiersin.org/article/10.3389/fpls.2018.00995/fullsoybeanflowering timematurityreproduction periodquantitative trait loci |