A chronic high-fat diet causes sperm head alterations in C57BL/6J mice
A chronic-positive energetic balance has been directly correlated with infertility in men, but the involved mechanisms remain unknown. Herein we investigated weather in a mouse model a chronic feeding with a diet supplemented with chicken fat affects sperm head morphology. To accomplish this, we fed...
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doaj-38d255968262469aaab57ad875f243a92020-11-25T02:14:03ZengElsevierHeliyon2405-84402019-11-01511e02868A chronic high-fat diet causes sperm head alterations in C57BL/6J miceA. Funes0T.E. Saez Lancellotti1L.D. Santillan2M.C. Della Vedova3M.A. Monclus4M.E. Cabrillana5S.E. Gomez Mejiba6D.C. Ramirez7M.W. Fornes8LIAM, Andrology Research Laboratory from Mendoza, IHEM-CCT-CONICET, National University of Cuyo and University of Aconcagua, Mendoza, 5500, ArgentinaLIAM, Andrology Research Laboratory from Mendoza, IHEM-CCT-CONICET, National University of Cuyo and University of Aconcagua, Mendoza, 5500, ArgentinaLETM, Laboratory of Experimental and Translational Medicine, IMIBIO-SL, CONICET, National University of San Luis, San Luis, 5700, ArgentinaLETM, Laboratory of Experimental and Translational Medicine, IMIBIO-SL, CONICET, National University of San Luis, San Luis, 5700, ArgentinaLIAM, Andrology Research Laboratory from Mendoza, IHEM-CCT-CONICET, National University of Cuyo and University of Aconcagua, Mendoza, 5500, ArgentinaLIAM, Andrology Research Laboratory from Mendoza, IHEM-CCT-CONICET, National University of Cuyo and University of Aconcagua, Mendoza, 5500, ArgentinaLET, Laboratory of Experimental Therapeutics, IMIBIO-SL, CONICET, National University of San Luis, San Luis, 5700, ArgentinaLETM, Laboratory of Experimental and Translational Medicine, IMIBIO-SL, CONICET, National University of San Luis, San Luis, 5700, Argentina; Corresponding author.LIAM, Andrology Research Laboratory from Mendoza, IHEM-CCT-CONICET, National University of Cuyo and University of Aconcagua, Mendoza, 5500, Argentina; Corresponding author.A chronic-positive energetic balance has been directly correlated with infertility in men, but the involved mechanisms remain unknown. Herein we investigated weather in a mouse model a chronic feeding with a diet supplemented with chicken fat affects sperm head morphology. To accomplish this, we fed mice for 16 weeks with either control food (low-fat diet, LFD) or control food supplemented with 22% chicken fat (high-fat diet, HFD). At the end of the feeding regimen, we measured: redox and inflammatory changes, cholesterol accumulation in testis and analyzed testicular morphological structure and ultra-structure and liver morphology. We found that the mice fed HFD resembled some features of the human metabolic syndrome, including systemic oxidative stress and inflammation, this group showed an increment in the following parameters; central adiposity (adiposity index: 1.07 ± 0.10 vs 2.26 ± 0.17), dyslipidemia (total cholesterol: 153.3 ± 2.6 vs 175.1 ± 8.08 mg/dL), insulin resistance (indirect Insulin resistance index, TG/HDL-c: 2.94 ± 0.33 vs 3.68 ± 0.15) and fatty liver. Increased cholesterol content measured by filipin was found in the testicles from HFD (fluorescence intensity increase to 50%), as well as an alteration of spermiogenesis. Most remarkably, a disorganized manchette-perinuclear ring complex and an altered morphology of the sperm head were observed in the spermatozoa of HFD-fed mice. These results add new information to our understanding about the mechanisms by which systemic oxidative stress and inflammation may influence sperm-head morphology and indirectly male fertility.http://www.sciencedirect.com/science/article/pii/S2405844019365272Cell biologyImmunologyMolecular biologyProteinsDietMale fertility |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
A. Funes T.E. Saez Lancellotti L.D. Santillan M.C. Della Vedova M.A. Monclus M.E. Cabrillana S.E. Gomez Mejiba D.C. Ramirez M.W. Fornes |
spellingShingle |
A. Funes T.E. Saez Lancellotti L.D. Santillan M.C. Della Vedova M.A. Monclus M.E. Cabrillana S.E. Gomez Mejiba D.C. Ramirez M.W. Fornes A chronic high-fat diet causes sperm head alterations in C57BL/6J mice Heliyon Cell biology Immunology Molecular biology Proteins Diet Male fertility |
author_facet |
A. Funes T.E. Saez Lancellotti L.D. Santillan M.C. Della Vedova M.A. Monclus M.E. Cabrillana S.E. Gomez Mejiba D.C. Ramirez M.W. Fornes |
author_sort |
A. Funes |
title |
A chronic high-fat diet causes sperm head alterations in C57BL/6J mice |
title_short |
A chronic high-fat diet causes sperm head alterations in C57BL/6J mice |
title_full |
A chronic high-fat diet causes sperm head alterations in C57BL/6J mice |
title_fullStr |
A chronic high-fat diet causes sperm head alterations in C57BL/6J mice |
title_full_unstemmed |
A chronic high-fat diet causes sperm head alterations in C57BL/6J mice |
title_sort |
chronic high-fat diet causes sperm head alterations in c57bl/6j mice |
publisher |
Elsevier |
series |
Heliyon |
issn |
2405-8440 |
publishDate |
2019-11-01 |
description |
A chronic-positive energetic balance has been directly correlated with infertility in men, but the involved mechanisms remain unknown. Herein we investigated weather in a mouse model a chronic feeding with a diet supplemented with chicken fat affects sperm head morphology. To accomplish this, we fed mice for 16 weeks with either control food (low-fat diet, LFD) or control food supplemented with 22% chicken fat (high-fat diet, HFD). At the end of the feeding regimen, we measured: redox and inflammatory changes, cholesterol accumulation in testis and analyzed testicular morphological structure and ultra-structure and liver morphology. We found that the mice fed HFD resembled some features of the human metabolic syndrome, including systemic oxidative stress and inflammation, this group showed an increment in the following parameters; central adiposity (adiposity index: 1.07 ± 0.10 vs 2.26 ± 0.17), dyslipidemia (total cholesterol: 153.3 ± 2.6 vs 175.1 ± 8.08 mg/dL), insulin resistance (indirect Insulin resistance index, TG/HDL-c: 2.94 ± 0.33 vs 3.68 ± 0.15) and fatty liver. Increased cholesterol content measured by filipin was found in the testicles from HFD (fluorescence intensity increase to 50%), as well as an alteration of spermiogenesis. Most remarkably, a disorganized manchette-perinuclear ring complex and an altered morphology of the sperm head were observed in the spermatozoa of HFD-fed mice. These results add new information to our understanding about the mechanisms by which systemic oxidative stress and inflammation may influence sperm-head morphology and indirectly male fertility. |
topic |
Cell biology Immunology Molecular biology Proteins Diet Male fertility |
url |
http://www.sciencedirect.com/science/article/pii/S2405844019365272 |
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