Loop-Mediated Isothermal Amplification Methods for Diagnosis of Bacterial Meningitis
The rapid, accurate, and efficient identification of an infectious disease is critical to ensure timely clinical treatment and prevention in public health settings. In 2015, meningitis caused by Streptococcus pneumoniae, Haemophilus influenzae, and Neisseria meningitidis was responsible for 379,200...
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2018-03-01
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doaj-38ec5d1d335e415cab914ea5f306420b2020-11-24T23:14:52ZengFrontiers Media S.A.Frontiers in Pediatrics2296-23602018-03-01610.3389/fped.2018.00057345618Loop-Mediated Isothermal Amplification Methods for Diagnosis of Bacterial MeningitisMitsuko Seki0Paul E. Kilgore1Eun Jin Kim2Eun Jin Kim3Makoto Ohnishi4Satoshi Hayakawa5Dong Wook Kim6Dong Wook Kim7Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, JapanDepartment of Pharmacy Practice, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI, United StatesDepartment of Pharmacy, College of Pharmacy, Hanyang University, Ansan, South KoreaInstitute of Pharmacological Research, Hanyang University, Ansan, South KoreaDepartment of Bacteriology I, National Institute of Infectious Diseases, Tokyo, JapanDivision of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, JapanDepartment of Pharmacy, College of Pharmacy, Hanyang University, Ansan, South KoreaInstitute of Pharmacological Research, Hanyang University, Ansan, South KoreaThe rapid, accurate, and efficient identification of an infectious disease is critical to ensure timely clinical treatment and prevention in public health settings. In 2015, meningitis caused by Streptococcus pneumoniae, Haemophilus influenzae, and Neisseria meningitidis was responsible for 379,200 (range: 322,700–444,700) deaths. Clinical features alone cannot determine whether bacterial meningitis is present; an analysis of cerebrospinal fluid (CSF) is essential. Loop-mediated isothermal amplification (LAMP) is a nucleic acid amplification method offering an alternative to polymerase chain reaction (PCR). LAMP-based assays for detection of three leading bacteria in CSF for diagnosis of meningitis have been established. The typing assays using LAMP for detection of meningococcal serogroups A, B, C, W, X, and Y as well as H. influenzae serotypes a, b, c, d, e, and f were launched. In comparative analysis of the meningitis pathogen assays, LAMP assays did not yield false negative results, and the detection rate of LAMP assays was superior compared with PCR or conventional culture methods. LAMP assays provide accurate and rapid test results to detect major bacterial meningitis pathogens. Accumulating evidence suggests that LAMP assays have the potential to provide urgently needed diagnostics for bacterial meningitis in resource-limited settings of both developed and developing countries.http://journal.frontiersin.org/article/10.3389/fped.2018.00057/fullloop-mediated isothermal amplificationmeningitiscerebrospinal fluidNeisseria meningitidisHaemophilus influenzaeStreptococcus pneumoniae |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mitsuko Seki Paul E. Kilgore Eun Jin Kim Eun Jin Kim Makoto Ohnishi Satoshi Hayakawa Dong Wook Kim Dong Wook Kim |
spellingShingle |
Mitsuko Seki Paul E. Kilgore Eun Jin Kim Eun Jin Kim Makoto Ohnishi Satoshi Hayakawa Dong Wook Kim Dong Wook Kim Loop-Mediated Isothermal Amplification Methods for Diagnosis of Bacterial Meningitis Frontiers in Pediatrics loop-mediated isothermal amplification meningitis cerebrospinal fluid Neisseria meningitidis Haemophilus influenzae Streptococcus pneumoniae |
author_facet |
Mitsuko Seki Paul E. Kilgore Eun Jin Kim Eun Jin Kim Makoto Ohnishi Satoshi Hayakawa Dong Wook Kim Dong Wook Kim |
author_sort |
Mitsuko Seki |
title |
Loop-Mediated Isothermal Amplification Methods for Diagnosis of Bacterial Meningitis |
title_short |
Loop-Mediated Isothermal Amplification Methods for Diagnosis of Bacterial Meningitis |
title_full |
Loop-Mediated Isothermal Amplification Methods for Diagnosis of Bacterial Meningitis |
title_fullStr |
Loop-Mediated Isothermal Amplification Methods for Diagnosis of Bacterial Meningitis |
title_full_unstemmed |
Loop-Mediated Isothermal Amplification Methods for Diagnosis of Bacterial Meningitis |
title_sort |
loop-mediated isothermal amplification methods for diagnosis of bacterial meningitis |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Pediatrics |
issn |
2296-2360 |
publishDate |
2018-03-01 |
description |
The rapid, accurate, and efficient identification of an infectious disease is critical to ensure timely clinical treatment and prevention in public health settings. In 2015, meningitis caused by Streptococcus pneumoniae, Haemophilus influenzae, and Neisseria meningitidis was responsible for 379,200 (range: 322,700–444,700) deaths. Clinical features alone cannot determine whether bacterial meningitis is present; an analysis of cerebrospinal fluid (CSF) is essential. Loop-mediated isothermal amplification (LAMP) is a nucleic acid amplification method offering an alternative to polymerase chain reaction (PCR). LAMP-based assays for detection of three leading bacteria in CSF for diagnosis of meningitis have been established. The typing assays using LAMP for detection of meningococcal serogroups A, B, C, W, X, and Y as well as H. influenzae serotypes a, b, c, d, e, and f were launched. In comparative analysis of the meningitis pathogen assays, LAMP assays did not yield false negative results, and the detection rate of LAMP assays was superior compared with PCR or conventional culture methods. LAMP assays provide accurate and rapid test results to detect major bacterial meningitis pathogens. Accumulating evidence suggests that LAMP assays have the potential to provide urgently needed diagnostics for bacterial meningitis in resource-limited settings of both developed and developing countries. |
topic |
loop-mediated isothermal amplification meningitis cerebrospinal fluid Neisseria meningitidis Haemophilus influenzae Streptococcus pneumoniae |
url |
http://journal.frontiersin.org/article/10.3389/fped.2018.00057/full |
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