SEQUENCING OF FLAX LIS-1 INSERTION SITE IN THE ALBIDUM GENOTYPE

• Main Authors: Jana Žiarovská, Katarína Ražná, Milan Bežo, Marek Schlosser
• Format: Article
• Language: English
• Published: Slovak University of Agriculture 2012-12-01
• Series: Journal of Microbiology, Biotechnology and Food Sciences
• Subjects: common flax; LIS-1 insertion site; sequence; PCR identification
• Online Access: http://www.jmbfs.org/wp-content/uploads/2012/12/jmbfs_0218_ziarovska.pdf

The paper presents a methodology of identifying the insertion site of LIS-1-1 (Linum Insertion Sequence 1) element in flax Albidum variety when growing under the in vitro combined with environmental stress conditions. Abiotic stress was induced by a reduced nutrient content in a growth medium. The LIS-1 insertion site amplification was reaLIS-1ed using the forward LIS-L: 5'-GGG CAG TTT AAC TGT AAC GAA - 3 'and revers LIS-R: 5'-GCT TGG ATT TAG ACT TGG CAA C - 3' primers by PCR. PCR product was sequenced by direct sequencing method to proove the nucleotide sequence for matching with database LIS-1 sequence. A comparison has been matched with the sequence of the amplified segment in the database for all nucleotides except the 11-position in the 5'-3 ' direction, where instead of the three adenine pair is a couple in the Albidum variety. Changes caused by mobile elements or insertion sequences result in common flax in variability that can be used for the purposes of development of effective marker identification or environment based markers development.