Isolation, Screening and Identification of Diatoms from Kermanshah Oil Refinery Wastewater Treatment Systems
Introduction: There are many oil refineries in Iran, so waste water should be treated. Algal remediation is one of the best methods. The class of Bacillariophyta (diatoms) has distinct ecological preferences and tolerances, making them useful indicators of contemporary ecological conditions. Isolati...
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doaj-38f075f762df48019a214b6aea48946c2020-11-24T21:53:33ZengUniversity of IsfahanBiological Journal of Microorganism2322-51732322-51812014-11-013114758Isolation, Screening and Identification of Diatoms from Kermanshah Oil Refinery Wastewater Treatment SystemsElham Mohammadian0Naser Karimi1Behnam Rasekh2HamidReza Ghasempour3Soma Dehlavi4 Introduction: There are many oil refineries in Iran, so waste water should be treated. Algal remediation is one of the best methods. The class of Bacillariophyta (diatoms) has distinct ecological preferences and tolerances, making them useful indicators of contemporary ecological conditions. Isolation and selection of microorganisms is the first step in bioremediation. Palmer assessed the tolerance of algal species to organic pollution, and incorporated the data into an organic pollution index for rating water quality. Biovolume is used as a measure of relative algal biomass. The present work was conducted to isolate and select the tolerance diatoms from Kermanshah Oil Refinery wastewater treatment systems. This helped to improve diatoms community of Iran and prepare a list of tolerance diatom in bioremediation. Materials and methods: Wastewater samples were collected aseptically at six points in and transferred to the laboratory. The serial diluted were prepared in BG broth, and then samples were cultured into Bristol agar and BG agar with chloramphenicol. Finally they were cultured in NA to assurance that there are no bacteria. The brown diatoms colonies were cultured in new plates. The samples were observed through microscope, and keys were used to classify them. Also biovolume according to geometric shapes (um3) were calculated. Results: There was no bacterial growth after 48 hours thus this method is an applied method in order to isolate diatoms. A variety of classes were observed such as Navicula, Nitzschia and Frustulia. Analysis of the biovolume revealed that the species of Fragilaria capucina has the maximum biovolume. Discussion and conclusion: This research revealed that the diatoms diversity in Kermanshah Oil Refinery wastewater treatment systems is low due to the high rate of organic pollution according to Palmer index. Also Pinnularia, Fragilaria can be used in the list of tolerance diatoms. http://uijs.ui.ac.ir/bjm//browse.php?a_code=A-10-264-1&slc_lang=en&sid=1Diatoms Kermanshah Oil Refinery Wastewater Screening |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Elham Mohammadian Naser Karimi Behnam Rasekh HamidReza Ghasempour Soma Dehlavi |
spellingShingle |
Elham Mohammadian Naser Karimi Behnam Rasekh HamidReza Ghasempour Soma Dehlavi Isolation, Screening and Identification of Diatoms from Kermanshah Oil Refinery Wastewater Treatment Systems Biological Journal of Microorganism Diatoms Kermanshah Oil Refinery Wastewater Screening |
author_facet |
Elham Mohammadian Naser Karimi Behnam Rasekh HamidReza Ghasempour Soma Dehlavi |
author_sort |
Elham Mohammadian |
title |
Isolation, Screening and Identification of Diatoms from Kermanshah Oil Refinery Wastewater Treatment Systems |
title_short |
Isolation, Screening and Identification of Diatoms from Kermanshah Oil Refinery Wastewater Treatment Systems |
title_full |
Isolation, Screening and Identification of Diatoms from Kermanshah Oil Refinery Wastewater Treatment Systems |
title_fullStr |
Isolation, Screening and Identification of Diatoms from Kermanshah Oil Refinery Wastewater Treatment Systems |
title_full_unstemmed |
Isolation, Screening and Identification of Diatoms from Kermanshah Oil Refinery Wastewater Treatment Systems |
title_sort |
isolation, screening and identification of diatoms from kermanshah oil refinery wastewater treatment systems |
publisher |
University of Isfahan |
series |
Biological Journal of Microorganism |
issn |
2322-5173 2322-5181 |
publishDate |
2014-11-01 |
description |
Introduction: There are many oil refineries in Iran, so waste water
should be treated. Algal remediation is one of the best methods. The class of
Bacillariophyta (diatoms) has distinct ecological preferences and tolerances,
making them useful indicators of contemporary ecological conditions. Isolation
and selection of microorganisms is the first step in bioremediation. Palmer
assessed the tolerance of algal species to organic pollution, and incorporated
the data into an organic pollution index for rating water quality. Biovolume is
used as a measure of relative algal biomass. The present work was conducted to
isolate and select the tolerance diatoms from Kermanshah Oil Refinery
wastewater treatment systems. This helped to improve diatoms community of Iran
and prepare a list of tolerance diatom in bioremediation.
Materials and
methods: Wastewater samples
were collected aseptically at six points in and transferred to the laboratory.
The serial diluted were prepared in BG broth, and then samples were cultured
into Bristol agar and BG agar with chloramphenicol. Finally they were cultured
in NA to assurance that there are no bacteria. The brown diatoms colonies were
cultured in new plates. The samples were observed through microscope, and keys
were used to classify them. Also biovolume according to geometric shapes (um3)
were calculated.
Results: There was no bacterial growth after 48 hours thus
this method is an applied method in order to isolate diatoms. A variety of
classes were observed such as Navicula, Nitzschia and Frustulia.
Analysis of the biovolume revealed that the species of Fragilaria capucina
has the maximum biovolume.
Discussion and conclusion: This research revealed that the diatoms diversity in Kermanshah Oil
Refinery wastewater treatment systems is low due to the high rate of organic
pollution according to Palmer index. Also Pinnularia, Fragilaria can be
used in the list of tolerance diatoms.
|
topic |
Diatoms Kermanshah Oil Refinery Wastewater Screening |
url |
http://uijs.ui.ac.ir/bjm//browse.php?a_code=A-10-264-1&slc_lang=en&sid=1 |
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