Porcine Epidemic Diarrhea Virus (PEDV) co-infection induced chlamydial persistence/stress does not require productive viral replication
Chlamydiae may exist at the site of infection in an alternative replicative form, called the aberrant body (AB). AB are produced during a viable but non-infectious developmental state termed persistence or chlamydial stress. As persistent/stressed chlamydiae: i) may contribute to chronic inflammatio...
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doaj-38fe489b7b0f436caa2afbf2c10cda332020-11-24T22:37:35ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882014-03-01410.3389/fcimb.2014.0002076691Porcine Epidemic Diarrhea Virus (PEDV) co-infection induced chlamydial persistence/stress does not require productive viral replicationNicole eBorel0Robert V Schoborg1University of ZurichQuillen College of MedicineChlamydiae may exist at the site of infection in an alternative replicative form, called the aberrant body (AB). AB are produced during a viable but non-infectious developmental state termed persistence or chlamydial stress. As persistent/stressed chlamydiae: i) may contribute to chronic inflammation observed in diseases like trachoma; and ii) are more resistant to current anti-chlamydial drugs of choice, it is critical to better understand this developmental stage. We previously demonstrated that porcine epidemic diarrhea virus (PEDV) co-infection induced C. pecorum persistence/stress in culture. One critical characteristic of persistence/stress is that the chlamydiae remain viable and can reenter the normal developmental cycle when the stressor is removed. Thus, we hypothesized that PEDV-induced persistence would be reversible if viral replication was inhibited. Therefore, we performed time course experiments in which Vero cells were C. pecorum/PEDV infected in the presence of cycloheximide (CHX), which inhibits viral but not chlamydial protein synthesis. CHX-exposure inhibited PEDV replication, but did not inhibit induction of C. pecorum persistence at 24 h post-PEDV infection, as indicated by AB formation and reduced production of infectious EBs. Interestingly, production of infectious EB resumed when CHX-exposed, co-infected cells were incubated 48 to 72 h post-PEDV co-infection. These data demonstrate that PEDV co-infection-induced chlamydial persistence/stress is reversible and suggest that this induction i) does not require viral replication in host cells; and iii) does not require de novo host or viral protein synthesis. These data also suggest that viral binding and/or entry may be required for this effect. Because the PEDV host cell receptor (CD13 or aminopeptidase N) stimulates cellular signaling pathways in the absence of PEDV infection, we suspect that PEDV co-infection might alter CD13 function and induce the chlamydiae to enter the persistent stahttp://journal.frontiersin.org/Journal/10.3389/fcimb.2014.00020/fullChlamydia pecorumchlamydial persistencechlamydial stress responsestressed chlamydiaePorcine Epidemic Diarrhea Virus (PEDV) |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nicole eBorel Robert V Schoborg |
spellingShingle |
Nicole eBorel Robert V Schoborg Porcine Epidemic Diarrhea Virus (PEDV) co-infection induced chlamydial persistence/stress does not require productive viral replication Frontiers in Cellular and Infection Microbiology Chlamydia pecorum chlamydial persistence chlamydial stress response stressed chlamydiae Porcine Epidemic Diarrhea Virus (PEDV) |
author_facet |
Nicole eBorel Robert V Schoborg |
author_sort |
Nicole eBorel |
title |
Porcine Epidemic Diarrhea Virus (PEDV) co-infection induced chlamydial persistence/stress does not require productive viral replication |
title_short |
Porcine Epidemic Diarrhea Virus (PEDV) co-infection induced chlamydial persistence/stress does not require productive viral replication |
title_full |
Porcine Epidemic Diarrhea Virus (PEDV) co-infection induced chlamydial persistence/stress does not require productive viral replication |
title_fullStr |
Porcine Epidemic Diarrhea Virus (PEDV) co-infection induced chlamydial persistence/stress does not require productive viral replication |
title_full_unstemmed |
Porcine Epidemic Diarrhea Virus (PEDV) co-infection induced chlamydial persistence/stress does not require productive viral replication |
title_sort |
porcine epidemic diarrhea virus (pedv) co-infection induced chlamydial persistence/stress does not require productive viral replication |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cellular and Infection Microbiology |
issn |
2235-2988 |
publishDate |
2014-03-01 |
description |
Chlamydiae may exist at the site of infection in an alternative replicative form, called the aberrant body (AB). AB are produced during a viable but non-infectious developmental state termed persistence or chlamydial stress. As persistent/stressed chlamydiae: i) may contribute to chronic inflammation observed in diseases like trachoma; and ii) are more resistant to current anti-chlamydial drugs of choice, it is critical to better understand this developmental stage. We previously demonstrated that porcine epidemic diarrhea virus (PEDV) co-infection induced C. pecorum persistence/stress in culture. One critical characteristic of persistence/stress is that the chlamydiae remain viable and can reenter the normal developmental cycle when the stressor is removed. Thus, we hypothesized that PEDV-induced persistence would be reversible if viral replication was inhibited. Therefore, we performed time course experiments in which Vero cells were C. pecorum/PEDV infected in the presence of cycloheximide (CHX), which inhibits viral but not chlamydial protein synthesis. CHX-exposure inhibited PEDV replication, but did not inhibit induction of C. pecorum persistence at 24 h post-PEDV infection, as indicated by AB formation and reduced production of infectious EBs. Interestingly, production of infectious EB resumed when CHX-exposed, co-infected cells were incubated 48 to 72 h post-PEDV co-infection. These data demonstrate that PEDV co-infection-induced chlamydial persistence/stress is reversible and suggest that this induction i) does not require viral replication in host cells; and iii) does not require de novo host or viral protein synthesis. These data also suggest that viral binding and/or entry may be required for this effect. Because the PEDV host cell receptor (CD13 or aminopeptidase N) stimulates cellular signaling pathways in the absence of PEDV infection, we suspect that PEDV co-infection might alter CD13 function and induce the chlamydiae to enter the persistent sta |
topic |
Chlamydia pecorum chlamydial persistence chlamydial stress response stressed chlamydiae Porcine Epidemic Diarrhea Virus (PEDV) |
url |
http://journal.frontiersin.org/Journal/10.3389/fcimb.2014.00020/full |
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