Protective Effects of Anti-IL17 on Acute Lung Injury Induced by LPS in Mice
Introduction: T helper 17 (Th17) has been implicated in a variety of inflammatory lung and immune system diseases. However, little is known about the expression and biological role of IL-17 in acute lung injury (ALI). We investigated the mechanisms involved in the effect of anti-IL17 in a model of l...
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doaj-390c3cf00f574eaa8022bb92847f99932020-11-24T21:11:59ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122018-10-01910.3389/fphar.2018.01021395091Protective Effects of Anti-IL17 on Acute Lung Injury Induced by LPS in MiceRenato Fraga Righetti0Renato Fraga Righetti1Tabata Maruyama dos Santos2Tabata Maruyama dos Santos3Leandro do Nascimento Camargo4Luciana Ritha Cássia Rolim Barbosa Aristóteles5Silvia Fukuzaki6Flávia Castro Ribas de Souza7Fernanda Paula Roncon Santana8Marcus Vinicius Rodrigues de Agrela9Maysa Mariana Cruz10Maria Isabel Cardoso Alonso-Vale11Isabella Santos Genaro12Isabella Santos Genaro13Beatriz Mangueira Saraiva-Romanholo14Beatriz Mangueira Saraiva-Romanholo15Edna Aparecida Leick16Milton de Arruda Martins17Carla Máximo Prado18Iolanda de Fátima Lopes Calvo Tibério19Faculdade de Medicina FMUSP, Universidade de São Paulo, São Paulo, BrazilHospital Sírio-Libanês, São Paulo, BrazilFaculdade de Medicina FMUSP, Universidade de São Paulo, São Paulo, BrazilHospital Sírio-Libanês, São Paulo, BrazilFaculdade de Medicina FMUSP, Universidade de São Paulo, São Paulo, BrazilFaculdade de Medicina FMUSP, Universidade de São Paulo, São Paulo, BrazilFaculdade de Medicina FMUSP, Universidade de São Paulo, São Paulo, BrazilFaculdade de Medicina FMUSP, Universidade de São Paulo, São Paulo, BrazilFaculdade de Medicina FMUSP, Universidade de São Paulo, São Paulo, BrazilFaculdade de Medicina FMUSP, Universidade de São Paulo, São Paulo, BrazilUniversidade Federal de São Paulo, Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Departamento de Ciências Biológicas, Diadema, São Paulo, BrazilUniversidade Federal de São Paulo, Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Departamento de Ciências Biológicas, Diadema, São Paulo, BrazilFaculdade de Medicina FMUSP, Universidade de São Paulo, São Paulo, BrazilPublic Employee of São Paulo Hospital (IAMSPE), São Paulo, BrazilFaculdade de Medicina FMUSP, Universidade de São Paulo, São Paulo, BrazilUniversity of City of São Paulo (UNICID), São Paulo, BrazilFaculdade de Medicina FMUSP, Universidade de São Paulo, São Paulo, BrazilFaculdade de Medicina FMUSP, Universidade de São Paulo, São Paulo, BrazilDepartment of Bioscience, Federal University of São Paulo, Santos, São Paulo, BrazilFaculdade de Medicina FMUSP, Universidade de São Paulo, São Paulo, BrazilIntroduction: T helper 17 (Th17) has been implicated in a variety of inflammatory lung and immune system diseases. However, little is known about the expression and biological role of IL-17 in acute lung injury (ALI). We investigated the mechanisms involved in the effect of anti-IL17 in a model of lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice.Methods: Mice were pre-treated with anti-IL17, 1h before saline/LPS intratracheal administration alongside non-treated controls and levels of exhaled nitric oxide (eNO), cytokine expression, extracellular matrix remodeling and oxidative stress, as well as immune cell counts in bronchoalveolar lavage fluid (BALF), and respiratory mechanics were assessed in lung tissue.Results: LPS instillation led to an increase in multiple cytokines, proteases, nuclear factor-κB, and Forkhead box P3 (FOXP3), eNO and regulators of the actomyosin cytoskeleton, the number of CD4+ and iNOS-positive cells as well as the number of neutrophils and macrophages in BALF, resistance and elastance of the respiratory system, ARG-1 gene expression, collagen fibers, and actin and 8-iso-PGF2α volume fractions. Pre-treatment with anti-IL17 led to a significant reduction in the level of all assessed factors.Conclusions: Anti-IL17 can protect the lungs from the inflammatory effects of LPS-induced ALI, primarily mediated by the reduced expression of cytokines and oxidative stress. This suggests that further studies using anti-IL17 in a treatment regime would be highly worthwhile.https://www.frontiersin.org/article/10.3389/fphar.2018.01021/fullacute lung injuryinflammation mediatorsinterleukin 17remodelingoxidative stress |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Renato Fraga Righetti Renato Fraga Righetti Tabata Maruyama dos Santos Tabata Maruyama dos Santos Leandro do Nascimento Camargo Luciana Ritha Cássia Rolim Barbosa Aristóteles Silvia Fukuzaki Flávia Castro Ribas de Souza Fernanda Paula Roncon Santana Marcus Vinicius Rodrigues de Agrela Maysa Mariana Cruz Maria Isabel Cardoso Alonso-Vale Isabella Santos Genaro Isabella Santos Genaro Beatriz Mangueira Saraiva-Romanholo Beatriz Mangueira Saraiva-Romanholo Edna Aparecida Leick Milton de Arruda Martins Carla Máximo Prado Iolanda de Fátima Lopes Calvo Tibério |
spellingShingle |
Renato Fraga Righetti Renato Fraga Righetti Tabata Maruyama dos Santos Tabata Maruyama dos Santos Leandro do Nascimento Camargo Luciana Ritha Cássia Rolim Barbosa Aristóteles Silvia Fukuzaki Flávia Castro Ribas de Souza Fernanda Paula Roncon Santana Marcus Vinicius Rodrigues de Agrela Maysa Mariana Cruz Maria Isabel Cardoso Alonso-Vale Isabella Santos Genaro Isabella Santos Genaro Beatriz Mangueira Saraiva-Romanholo Beatriz Mangueira Saraiva-Romanholo Edna Aparecida Leick Milton de Arruda Martins Carla Máximo Prado Iolanda de Fátima Lopes Calvo Tibério Protective Effects of Anti-IL17 on Acute Lung Injury Induced by LPS in Mice Frontiers in Pharmacology acute lung injury inflammation mediators interleukin 17 remodeling oxidative stress |
author_facet |
Renato Fraga Righetti Renato Fraga Righetti Tabata Maruyama dos Santos Tabata Maruyama dos Santos Leandro do Nascimento Camargo Luciana Ritha Cássia Rolim Barbosa Aristóteles Silvia Fukuzaki Flávia Castro Ribas de Souza Fernanda Paula Roncon Santana Marcus Vinicius Rodrigues de Agrela Maysa Mariana Cruz Maria Isabel Cardoso Alonso-Vale Isabella Santos Genaro Isabella Santos Genaro Beatriz Mangueira Saraiva-Romanholo Beatriz Mangueira Saraiva-Romanholo Edna Aparecida Leick Milton de Arruda Martins Carla Máximo Prado Iolanda de Fátima Lopes Calvo Tibério |
author_sort |
Renato Fraga Righetti |
title |
Protective Effects of Anti-IL17 on Acute Lung Injury Induced by LPS in Mice |
title_short |
Protective Effects of Anti-IL17 on Acute Lung Injury Induced by LPS in Mice |
title_full |
Protective Effects of Anti-IL17 on Acute Lung Injury Induced by LPS in Mice |
title_fullStr |
Protective Effects of Anti-IL17 on Acute Lung Injury Induced by LPS in Mice |
title_full_unstemmed |
Protective Effects of Anti-IL17 on Acute Lung Injury Induced by LPS in Mice |
title_sort |
protective effects of anti-il17 on acute lung injury induced by lps in mice |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Pharmacology |
issn |
1663-9812 |
publishDate |
2018-10-01 |
description |
Introduction: T helper 17 (Th17) has been implicated in a variety of inflammatory lung and immune system diseases. However, little is known about the expression and biological role of IL-17 in acute lung injury (ALI). We investigated the mechanisms involved in the effect of anti-IL17 in a model of lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice.Methods: Mice were pre-treated with anti-IL17, 1h before saline/LPS intratracheal administration alongside non-treated controls and levels of exhaled nitric oxide (eNO), cytokine expression, extracellular matrix remodeling and oxidative stress, as well as immune cell counts in bronchoalveolar lavage fluid (BALF), and respiratory mechanics were assessed in lung tissue.Results: LPS instillation led to an increase in multiple cytokines, proteases, nuclear factor-κB, and Forkhead box P3 (FOXP3), eNO and regulators of the actomyosin cytoskeleton, the number of CD4+ and iNOS-positive cells as well as the number of neutrophils and macrophages in BALF, resistance and elastance of the respiratory system, ARG-1 gene expression, collagen fibers, and actin and 8-iso-PGF2α volume fractions. Pre-treatment with anti-IL17 led to a significant reduction in the level of all assessed factors.Conclusions: Anti-IL17 can protect the lungs from the inflammatory effects of LPS-induced ALI, primarily mediated by the reduced expression of cytokines and oxidative stress. This suggests that further studies using anti-IL17 in a treatment regime would be highly worthwhile. |
topic |
acute lung injury inflammation mediators interleukin 17 remodeling oxidative stress |
url |
https://www.frontiersin.org/article/10.3389/fphar.2018.01021/full |
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