Diabetes sepsis on Wistar rat strain (Rattus norvegicus) induced by streptozotocin and bacteria Staphylococcus aureus
Background and Aim: Sepsis is characterized by loss of control of the inflammatory response, which can be triggered by various microorganisms and toxic secretions. The mortality rate increases due to impaired endothelial function caused dysfunctional organ systems. Diabetes is closely related to sep...
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doaj-394d093725034125bdf836ebee51663f2021-08-02T16:49:06ZengVeterinary WorldVeterinary World0972-89882231-09162019-06-0112684985410.14202/vetworld.2019.849-854Diabetes sepsis on Wistar rat strain (Rattus norvegicus) induced by streptozotocin and bacteria Staphylococcus aureusDahliatul Qosimah0Dhita Evi Aryani1Ma. Asuncion Guiang Beltran2Aulanni'am Aulanni'am3Laboratory of Microbiology and Immunology, Faculty of Veterinary Medicine, Brawijaya University, Indonesia.Laboratory of Pharmacology, Faculty of Veterinary Medicine, Brawijaya University, Indonesia.Department of Microbiology and Public Health , College of Veterinary Medicine, Tarlac Agricultural University, Camiling, Tarlac, Philippines.Laboratory of Biochemical, Faculty of Veterinary Medicine, Brawijaya University, Indonesia.Background and Aim: Sepsis is characterized by loss of control of the inflammatory response, which can be triggered by various microorganisms and toxic secretions. The mortality rate increases due to impaired endothelial function caused dysfunctional organ systems. Diabetes is closely related to sepsis. The study aimed to determine the method of using animal models of sepsis diabetes through a combination of streptozotocin (STZ) and Staphylococcus aureus infection based on biological marker parameters. Materials and Methods: A total of 30 male Wistar rats of 2.5-3 months old weighing approximately 150-250 g body weight (BW) divided into six treatment groups with five replications per group were used in the study. Treatment A was negative control (healthy rats) and Treatment B was the positive control (with diabetes) where rats were given STZ dose at 45 mg/kg BW on day 8 intraperitoneally (IP). The blood glucose was measured on day 10, Treatment C was a positive control (bacteria), rats inoculated with S. aureus with a concentration of 108 CFU/mL on day 8 given IP and observed sepsis conditions on day 10th. Treatment group (D, E, and F): Rats given STZ dose at 45 mg/kg BW on day 8th by IP and measured blood glucose on day 10th, then inoculated with S. aureus with different concentrations of 105 CFU/mL, 106 CFU/mL, and 107 CFU/mL on the 10th day, respectively, and were later observed the condition of sepsis on day 12th. Data on diabetes bacteremia were quantitative used blood glucose levels, the bacterial count, and C-reactive protein (CRP) and were analyzed using the one-way analysis of variance test with a confidence level of 95%. Physical examination (temperature and respiration) is qualitative. Results: Physical examination showed that all treatments had a normal temperature, an increased pulse in Groups D, E, and F and a decrease in respiratory rate in the treatment of E and F, the bacteria found in the vital organs in all groups, and CRP levels were not significantly different at all. Conclusion: Animal model of diabetes sepsis can be observed through a combination of pancreas damage, and respiration, the bacteria in the vital organs.http://www.veterinaryworld.org/Vol.12/June-2019/19.pdfanimal modeldiabetesinflammationsepsis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Dahliatul Qosimah Dhita Evi Aryani Ma. Asuncion Guiang Beltran Aulanni'am Aulanni'am |
spellingShingle |
Dahliatul Qosimah Dhita Evi Aryani Ma. Asuncion Guiang Beltran Aulanni'am Aulanni'am Diabetes sepsis on Wistar rat strain (Rattus norvegicus) induced by streptozotocin and bacteria Staphylococcus aureus Veterinary World animal model diabetes inflammation sepsis |
author_facet |
Dahliatul Qosimah Dhita Evi Aryani Ma. Asuncion Guiang Beltran Aulanni'am Aulanni'am |
author_sort |
Dahliatul Qosimah |
title |
Diabetes sepsis on Wistar rat strain (Rattus norvegicus) induced by streptozotocin and bacteria Staphylococcus aureus |
title_short |
Diabetes sepsis on Wistar rat strain (Rattus norvegicus) induced by streptozotocin and bacteria Staphylococcus aureus |
title_full |
Diabetes sepsis on Wistar rat strain (Rattus norvegicus) induced by streptozotocin and bacteria Staphylococcus aureus |
title_fullStr |
Diabetes sepsis on Wistar rat strain (Rattus norvegicus) induced by streptozotocin and bacteria Staphylococcus aureus |
title_full_unstemmed |
Diabetes sepsis on Wistar rat strain (Rattus norvegicus) induced by streptozotocin and bacteria Staphylococcus aureus |
title_sort |
diabetes sepsis on wistar rat strain (rattus norvegicus) induced by streptozotocin and bacteria staphylococcus aureus |
publisher |
Veterinary World |
series |
Veterinary World |
issn |
0972-8988 2231-0916 |
publishDate |
2019-06-01 |
description |
Background and Aim: Sepsis is characterized by loss of control of the inflammatory response, which can be triggered by various microorganisms and toxic secretions. The mortality rate increases due to impaired endothelial function caused dysfunctional organ systems. Diabetes is closely related to sepsis. The study aimed to determine the method of using animal models of sepsis diabetes through a combination of streptozotocin (STZ) and Staphylococcus aureus infection based on biological marker parameters.
Materials and Methods: A total of 30 male Wistar rats of 2.5-3 months old weighing approximately 150-250 g body weight (BW) divided into six treatment groups with five replications per group were used in the study. Treatment A was negative control (healthy rats) and Treatment B was the positive control (with diabetes) where rats were given STZ dose at 45 mg/kg BW on day 8 intraperitoneally (IP). The blood glucose was measured on day 10, Treatment C was a positive control (bacteria), rats inoculated with S. aureus with a concentration of 108 CFU/mL on day 8 given IP and observed sepsis conditions on day 10th. Treatment group (D, E, and F): Rats given STZ dose at 45 mg/kg BW on day 8th by IP and measured blood glucose on day 10th, then inoculated with S. aureus with different concentrations of 105 CFU/mL, 106 CFU/mL, and 107 CFU/mL on the 10th day, respectively, and were later observed the condition of sepsis on day 12th. Data on diabetes bacteremia were quantitative used blood glucose levels, the bacterial count, and C-reactive protein (CRP) and were analyzed using the one-way analysis of variance test with a confidence level of 95%. Physical examination (temperature and respiration) is qualitative.
Results: Physical examination showed that all treatments had a normal temperature, an increased pulse in Groups D, E, and F and a decrease in respiratory rate in the treatment of E and F, the bacteria found in the vital organs in all groups, and CRP levels were not significantly different at all.
Conclusion: Animal model of diabetes sepsis can be observed through a combination of pancreas damage, and respiration, the bacteria in the vital organs. |
topic |
animal model diabetes inflammation sepsis |
url |
http://www.veterinaryworld.org/Vol.12/June-2019/19.pdf |
work_keys_str_mv |
AT dahliatulqosimah diabetessepsisonwistarratstrainrattusnorvegicusinducedbystreptozotocinandbacteriastaphylococcusaureus AT dhitaeviaryani diabetessepsisonwistarratstrainrattusnorvegicusinducedbystreptozotocinandbacteriastaphylococcusaureus AT maasuncionguiangbeltran diabetessepsisonwistarratstrainrattusnorvegicusinducedbystreptozotocinandbacteriastaphylococcusaureus AT aulanniamaulanniam diabetessepsisonwistarratstrainrattusnorvegicusinducedbystreptozotocinandbacteriastaphylococcusaureus |
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