Effects of MRI Contrast Agents on the Stem Cell Phenotype
The ultimate therapy for ischemic stroke is restoration of blood supply in the ischemic region and regeneration of lost neural cells. This might be achieved by transplanting cells that differentiate into vascular or neuronal cell types, or secrete trophic factors that enhance self-renewal, recruitme...
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doaj-39533dc99cf848cbb55668e346cf6b452020-11-25T03:38:22ZengSAGE PublishingCell Transplantation0963-68971555-38922010-08-011910.3727/096368910X494623Effects of MRI Contrast Agents on the Stem Cell PhenotypeAnnelies Crabbe0Caroline Vandeputte1Tom Dresselaers2Angel Ayuso Sacido3Jose Manuel Garcia Verdugo4Jeroen Eyckmans5Frank P. Luyten6Koen Van Laere7Catherine M. Verfaillie8Uwe Himmelreich9Stem Cell Institute, K.U. Leuven, Leuven, BelgiumDivision of Nuclear Medicine, K.U. Leuven, Leuven, BelgiumBiomedical NMR Unit/MOSAIC, K.U. Leuven, Leuven, BelgiumCentro de Investigación Príncipe Felipe, CIBERNED, Universidad de Valencia, Valencia, SpainCentro de Investigación Príncipe Felipe, CIBERNED, Universidad de Valencia, Valencia, SpainLaboratory for Skeletal development and Joint Disorders, K.U. Leuven, Leuven, BelgiumLaboratory for Skeletal development and Joint Disorders, K.U. Leuven, Leuven, BelgiumDivision of Nuclear Medicine, K.U. Leuven, Leuven, BelgiumStem Cell Institute, K.U. Leuven, Leuven, BelgiumBiomedical NMR Unit/MOSAIC, K.U. Leuven, Leuven, BelgiumThe ultimate therapy for ischemic stroke is restoration of blood supply in the ischemic region and regeneration of lost neural cells. This might be achieved by transplanting cells that differentiate into vascular or neuronal cell types, or secrete trophic factors that enhance self-renewal, recruitment, long-term survival, and functional integration of endogenous stem/progenitor cells. Experimental stroke models have been developed to determine potential beneficial effect of stem/progenitor cell-based therapies. To follow the fate of grafted cells in vivo, a number of noninvasive imaging approaches have been developed. Magnetic resonance imaging (MRI) is a high-resolution, clinically relevant method allowing in vivo monitoring of cells labeled with contrast agents. In this study, labeling efficiency of three different stem cell populations [mouse embryonic stem cells (mESC), rat multipotent adult progenitor cells (rMAPC), and mouse mesenchymal stem cells (mMSC)] with three different (ultra)small superparamagnetic iron oxide [(U)SPIO] particles (Resovist®, Endorem®, Sinerem®) was compared. Labeling efficiency with Resovist® and Endorem® differed significantly between the different stem cells. Labeling with (U)SPIOs in the range that allows detection of cells by in vivo MRI did not affect differentiation of stem cells when labeled with concentrations of particles needed for MRI-based visualization. Finally, we demonstrated that labeled rMAPC could be detected in vivo and that labeling did not interfere with their migration. We conclude that successful use of (U)SPIOs for MRI-based visualization will require assessment of the optimal (U)SPIO for each individual (stem) cell population to ensure the most sensitive detection without associated toxicity.https://doi.org/10.3727/096368910X494623 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Annelies Crabbe Caroline Vandeputte Tom Dresselaers Angel Ayuso Sacido Jose Manuel Garcia Verdugo Jeroen Eyckmans Frank P. Luyten Koen Van Laere Catherine M. Verfaillie Uwe Himmelreich |
spellingShingle |
Annelies Crabbe Caroline Vandeputte Tom Dresselaers Angel Ayuso Sacido Jose Manuel Garcia Verdugo Jeroen Eyckmans Frank P. Luyten Koen Van Laere Catherine M. Verfaillie Uwe Himmelreich Effects of MRI Contrast Agents on the Stem Cell Phenotype Cell Transplantation |
author_facet |
Annelies Crabbe Caroline Vandeputte Tom Dresselaers Angel Ayuso Sacido Jose Manuel Garcia Verdugo Jeroen Eyckmans Frank P. Luyten Koen Van Laere Catherine M. Verfaillie Uwe Himmelreich |
author_sort |
Annelies Crabbe |
title |
Effects of MRI Contrast Agents on the Stem Cell Phenotype |
title_short |
Effects of MRI Contrast Agents on the Stem Cell Phenotype |
title_full |
Effects of MRI Contrast Agents on the Stem Cell Phenotype |
title_fullStr |
Effects of MRI Contrast Agents on the Stem Cell Phenotype |
title_full_unstemmed |
Effects of MRI Contrast Agents on the Stem Cell Phenotype |
title_sort |
effects of mri contrast agents on the stem cell phenotype |
publisher |
SAGE Publishing |
series |
Cell Transplantation |
issn |
0963-6897 1555-3892 |
publishDate |
2010-08-01 |
description |
The ultimate therapy for ischemic stroke is restoration of blood supply in the ischemic region and regeneration of lost neural cells. This might be achieved by transplanting cells that differentiate into vascular or neuronal cell types, or secrete trophic factors that enhance self-renewal, recruitment, long-term survival, and functional integration of endogenous stem/progenitor cells. Experimental stroke models have been developed to determine potential beneficial effect of stem/progenitor cell-based therapies. To follow the fate of grafted cells in vivo, a number of noninvasive imaging approaches have been developed. Magnetic resonance imaging (MRI) is a high-resolution, clinically relevant method allowing in vivo monitoring of cells labeled with contrast agents. In this study, labeling efficiency of three different stem cell populations [mouse embryonic stem cells (mESC), rat multipotent adult progenitor cells (rMAPC), and mouse mesenchymal stem cells (mMSC)] with three different (ultra)small superparamagnetic iron oxide [(U)SPIO] particles (Resovist®, Endorem®, Sinerem®) was compared. Labeling efficiency with Resovist® and Endorem® differed significantly between the different stem cells. Labeling with (U)SPIOs in the range that allows detection of cells by in vivo MRI did not affect differentiation of stem cells when labeled with concentrations of particles needed for MRI-based visualization. Finally, we demonstrated that labeled rMAPC could be detected in vivo and that labeling did not interfere with their migration. We conclude that successful use of (U)SPIOs for MRI-based visualization will require assessment of the optimal (U)SPIO for each individual (stem) cell population to ensure the most sensitive detection without associated toxicity. |
url |
https://doi.org/10.3727/096368910X494623 |
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