Co-Treatment with Cefotaxime and High-Fructose Diet Inducing Nonalcoholic Fatty Liver Disease and Gut Microbial Dysbiosis in Mice
<b> </b>High fructose diet causes metabolic syndrome and induces host gut microbial dysbiosis and related obesity and nonalcoholic fatty liver disease (NAFLD). Several antibiotic treatments could prevent fatty liver. However, there are studies that have demonstrated that a high-fructose...
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doaj-3982f38352924a11b7af5c72e4c112072021-03-01T00:00:05ZengMDPI AGProcesses2227-97172021-02-01943443410.3390/pr9030434Co-Treatment with Cefotaxime and High-Fructose Diet Inducing Nonalcoholic Fatty Liver Disease and Gut Microbial Dysbiosis in MiceYen-Peng Lee0Chien-Chao Chiu1Yi-Hsun Chen2Wen-Ching Huang3Yu-Chih Wang4Ching-Feng Chiu5Tien-Jen Lin6Shao-Wen Hung7Ju-Yun Liu8Hsiao-Li Chuang9Graduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University, Taichung 400, TaiwanAnimal Technology Research Center, Division of Animal Industry, Agricultural Technology Research Institute, Xiangshan, Hsinchu 300, TaiwanDepartment of Internal Medicine, College of Medicine, National Taiwan University, Taipei 222, TaiwanDepartment of Exercise and Health Science, National Taipei University of Nursing and Health Sciences, Taipei 222, TaiwanGraduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University, Taichung 400, TaiwanGraduate Institute of Metabolism and Obesity Sciences, College of Nutrition, Taipei Medical University, Taipei 222, TaiwanDepartment of Surgery, Division of Neurosurgery, School of Medicine, College of Medicine, Taipei Medical University, Taipei 222, TaiwanAnimal Technology Research Center, Division of Animal Industry, Agricultural Technology Research Institute, Xiangshan, Hsinchu 300, TaiwanNational Laboratory Animal Center, National Applied Research Laboratories, Taipei 222, TaiwanNational Laboratory Animal Center, National Applied Research Laboratories, Taipei 222, Taiwan<b> </b>High fructose diet causes metabolic syndrome and induces host gut microbial dysbiosis and related obesity and nonalcoholic fatty liver disease (NAFLD). Several antibiotic treatments could prevent fatty liver. However, there are studies that have demonstrated that a high-fructose diet could influence the gut microbial dysbiosis and induce fatty liver. The purpose of this study was performed to partially modify the gut bacterial composition with a single cefotaxime treatment, which might affect the fructose-induced NAFLD severity. The C57BL/6JNarl male mice were divided into four groups including vehicle/chow diet (VE-CD), vehicle/high-fructose diet (VE-FD), antibiotic (cefotaxime (CF))/CD, and CF/FD. The results showed that body weight gain, moderate hepatic steatosis severity, epididymal white adipose tissue hypertrophy, and insulin resistance occurrence with NAFLD-related symptoms were observed only in the CF-FD group. The raised protein expression of hepatic lipogenesis was observed in the CF-FD group, but lipolysis protein expression was no difference. The diversity and composition of microbiota were significantly reduced in the CF-FD group. The <i>Erysipelatoclostridium</i>, <i>Enterobacteriaceae</i>, <i>Lachnospiraceae</i><i>,</i> and <i>Escherichia Shigella</i> were in increased abundance in the feces of CF-FD group compared with VE-FD group. The novel model reveals that particular antibiotics such as cefotaxime co-treatment with high-fructose diet may affect the gut microbiota accelerating the NAFLD and obesity.https://www.mdpi.com/2227-9717/9/3/434cefotaximehigh-fructose dietnonalcoholic fatty liver diseasegut microbial |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yen-Peng Lee Chien-Chao Chiu Yi-Hsun Chen Wen-Ching Huang Yu-Chih Wang Ching-Feng Chiu Tien-Jen Lin Shao-Wen Hung Ju-Yun Liu Hsiao-Li Chuang |
spellingShingle |
Yen-Peng Lee Chien-Chao Chiu Yi-Hsun Chen Wen-Ching Huang Yu-Chih Wang Ching-Feng Chiu Tien-Jen Lin Shao-Wen Hung Ju-Yun Liu Hsiao-Li Chuang Co-Treatment with Cefotaxime and High-Fructose Diet Inducing Nonalcoholic Fatty Liver Disease and Gut Microbial Dysbiosis in Mice Processes cefotaxime high-fructose diet nonalcoholic fatty liver disease gut microbial |
author_facet |
Yen-Peng Lee Chien-Chao Chiu Yi-Hsun Chen Wen-Ching Huang Yu-Chih Wang Ching-Feng Chiu Tien-Jen Lin Shao-Wen Hung Ju-Yun Liu Hsiao-Li Chuang |
author_sort |
Yen-Peng Lee |
title |
Co-Treatment with Cefotaxime and High-Fructose Diet Inducing Nonalcoholic Fatty Liver Disease and Gut Microbial Dysbiosis in Mice |
title_short |
Co-Treatment with Cefotaxime and High-Fructose Diet Inducing Nonalcoholic Fatty Liver Disease and Gut Microbial Dysbiosis in Mice |
title_full |
Co-Treatment with Cefotaxime and High-Fructose Diet Inducing Nonalcoholic Fatty Liver Disease and Gut Microbial Dysbiosis in Mice |
title_fullStr |
Co-Treatment with Cefotaxime and High-Fructose Diet Inducing Nonalcoholic Fatty Liver Disease and Gut Microbial Dysbiosis in Mice |
title_full_unstemmed |
Co-Treatment with Cefotaxime and High-Fructose Diet Inducing Nonalcoholic Fatty Liver Disease and Gut Microbial Dysbiosis in Mice |
title_sort |
co-treatment with cefotaxime and high-fructose diet inducing nonalcoholic fatty liver disease and gut microbial dysbiosis in mice |
publisher |
MDPI AG |
series |
Processes |
issn |
2227-9717 |
publishDate |
2021-02-01 |
description |
<b> </b>High fructose diet causes metabolic syndrome and induces host gut microbial dysbiosis and related obesity and nonalcoholic fatty liver disease (NAFLD). Several antibiotic treatments could prevent fatty liver. However, there are studies that have demonstrated that a high-fructose diet could influence the gut microbial dysbiosis and induce fatty liver. The purpose of this study was performed to partially modify the gut bacterial composition with a single cefotaxime treatment, which might affect the fructose-induced NAFLD severity. The C57BL/6JNarl male mice were divided into four groups including vehicle/chow diet (VE-CD), vehicle/high-fructose diet (VE-FD), antibiotic (cefotaxime (CF))/CD, and CF/FD. The results showed that body weight gain, moderate hepatic steatosis severity, epididymal white adipose tissue hypertrophy, and insulin resistance occurrence with NAFLD-related symptoms were observed only in the CF-FD group. The raised protein expression of hepatic lipogenesis was observed in the CF-FD group, but lipolysis protein expression was no difference. The diversity and composition of microbiota were significantly reduced in the CF-FD group. The <i>Erysipelatoclostridium</i>, <i>Enterobacteriaceae</i>, <i>Lachnospiraceae</i><i>,</i> and <i>Escherichia Shigella</i> were in increased abundance in the feces of CF-FD group compared with VE-FD group. The novel model reveals that particular antibiotics such as cefotaxime co-treatment with high-fructose diet may affect the gut microbiota accelerating the NAFLD and obesity. |
topic |
cefotaxime high-fructose diet nonalcoholic fatty liver disease gut microbial |
url |
https://www.mdpi.com/2227-9717/9/3/434 |
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