| Summary: | <p>Vitamin A Deficiency (VAD) is associated with significant morbidity and mortality from common childhood infections and is the world’s leading preventable cause of childhood blindness. Studies showed that <em>carotenoid is one of the promissed vitamin A source. H</em>owever <em> the studies on carotenoid from yellow banana peel and its potential as a natural source of vitamin A has not been widely reported.</em> This study was conducted to measure the blood serum retinol levels of rats after administration of β-carotene from yellow ambon banana peel. This was an experimental study with post test only control group design, with sample size of 18 rats with age 1 month, devided into 3 groups. β-carotene dose administered based on the dose of red capsules vitamin A are (200,000 doses SI) for toddlers aged 12-59 months. Serum retinol levels were measured using a spectrophotometer according metide. This present study showed that the blood serum level in group treated with of β - carotene from yellow ambon banana peel (28.35 ± 1.61 mg/ dL ), was significantly different (p < 0.05) from that of control group ( 22.08 ± 1.35 mg /dL ). β-carotene from yellow ambon banana peel are potential as provitamin A.</p>
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